Semi-Automatic Segmentation of Cone Beam Computed Tomography Datasets for Volume Measurements of Equine Cheek Teeth

Abstract

The volumes of equine teeth may change considerably over time for several reasons including domestication, routine dental floating, and the hypsodont and anelodont nature of the teeth. Cone beam computed tomography (CBCT) of the head is routinely performed in standing horses and, in this proof of concept study, the feasibility of measuring tooth volume from CBCT datasets was determined. The CBCT images of 5 equine cadaver cheek teeth were segmented with a software 3-dimensional (3D) Slicer using a predefined protocol, corrected manually, and re-assembled into a 3D model. Individual tooth volume (V_S) was calculated from the model. After extraction, the volumes were also measured using the “gold-standard” water displacement method (V_W) for comparison. The V_S of 77 teeth ranged from 7114 to 42,300 mm³ which strongly correlated with V_W (r = 0.99)_, and on average V_S was 6.1% less than V_W. There was no significant difference in V_S between the right and left arcades in individual animals. Maxillary cheek tooth volume was on average 40% larger than it was for mandibular counterparts. Semi-automatic image segmentation of equine cheek teeth from CBCT data is feasible and accurate but requires some manual intervention. This preliminary study provides initial data on the volume of equine cheek teeth and creates new possibilities for future in vivo studies.