This study presents data to support the hypothesis that a major portion of the coding sequence of the amelogenin gene may have arisen by tandem duplication of internal sequences which as a consequence has introduced several additional potential RNA splice acceptor sites into the sequence. This duplication of splice sites has led to an increase in the heterogeneity of amelogenin forms found in developing enamel. By screening a rat enamel organ cDNA library for alternatively spliced products, it appears that as much as 20% of the amelogenin mRNA molecules may be alternatively spliced forms.
Aoba T., Tanabe T., Moreno EC (1987). Function of amelogenins on porcine enamel mineralization during the secretory stage of amelogenesis. Adv Dent Res1:252-260.
2.
Bonass WA, Robinson PA, Kirkham J., Shore RC, Robinson C. (1994a). Molecular cloning and DNA sequence of rat amelogenin and a comparative analysis of mammalian sequence divergence. Biochem Biophys Res Commun198:755-763.
3.
Bonass WA, Kirkham J., Brookes SJ, Shore RC, Robinson C. (1994b). Isolation and characterisation of an alternatively-spliced rat amelogenin cDNA: LRAP-a highly conserved, functional alternatively-spliced amelogenin?Biochim Biophys Acta1219:690-692.
4.
Brookes SJ, Robinson C., Kirkham J.Bonass WA (1995). Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Biol40:1-14.
5.
Catalano-Sherman J., Palmon A., Burstein Y., Deutsch D. (1993). Amino acid sequence of a major human amelogenin protein employing Edman degradation and cDNA sequencing. J Dent Res72:1566- 1572.
6.
DenBesten PK, Li RS (1992). Characterization of amelogenin mRNA from secretory- and maturation-stage rat incisor enamel. Arch Oral Biol37:1097-1100.
7.
Fincham AG, Belcourt AB, Termine JD (1982). Changing patterns of enamel matrix proteins in the developing bovine tooth. Caries Res16 :64-71.
8.
Fincham AG, Belcourt AB, Termine JD, Butler WT, Cothran WC (1983). Amelogenins-Sequence homologies in enamel-matrix proteins from three mammalian species. Biochem J211:149-154.
9.
Fincham AG, Hu Y., Lau EC, Slavkin HC, Snead ML (1991). Amelogenin post-secretory processing during biomineralization in the post natal mouse molar tooth. Arch Oral Biol36:305-317.
10.
Gibson C., Golub E., Herold R., Risser M., Ding W., Shimokawa H., et al. (1991a). Structure and expression of the bovine amelogenin gene. Biochemistry30:1075-1079.
11.
Gibson CW, Golub E., Ding W., Shimokawa H., Young M., Termine J., et al. (1991b). Identification of the leucine-rich amelogenin peptide (LRAP) as the translation product of an alternatively spliced transcript . Biochem Biophys Res Commun174:1306-1312.
12.
Gibson CW, Golub EE, Abrams WR, Shen G., Ding W., Rosenbloom J. (1992). Bovine amelogenin message heterogeneity: alternative splicing and Y-chromosomal gene transcription. Biochemistry31:8384-8388.
13.
Glimcher MJ, Brickley-Parsons D., Levine PT (1977). Studies of enamel proteins during maturation. Calcif Tissue Int24:259-270.
14.
Herold RC, Graver HT, Christner P. (1980). Immunohistochemical localization of amelogenins in enameloid of lower vertebrate teeth. Science207:1357-1358.
15.
Lau EC, Simmer JP, Bringas P., Hsu DDJ, Hu CC, Zeichner-David M., et al. (1992). Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity. Biochem Biophys Res Commun188:1253-1260.
16.
Nakahori Y., Takenaka O., Nakagome Y. (1991). A human X-Y homologous region encodes amelogenin . Genomics9:264-269.
17.
Robinson C., Fuchs P., Deutsch D., Weatherell JA (1978). Four chemically distinct stages in developing enamel from bovine incisor teeth. Caries Res12:1-11.
18.
Robinson C., Kirkham J., Shore RC (1992). Extracellular matrix of enamel and the ameloblast . Epith Cell Biol1:90-97.
19.
Salido EC, Yen PH, Koprivnikar K., Yu LC, Shapiro LJ (1992). The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes. Am J Hum Genet50:303-316.
20.
Shimokawa H., Sobell ME, Sasaki M., Termine JD, Young MF (1987). Heterogeneity of amelogenin mRNA in the bovine tooth germ. J Biol Chem262:4042-4047.
21.
Smith CW, Patton JG, Nadal-Ginard B. (1989). Alternative splicing in the control of gene expression . Ann Rev Genet23:527-577.
22.
Yamakoshi Y., Tanabe T., Fukae M., Shimizu M. (1994). Porcine amelogenins. Calcif Tissue Int554:69-75.
23.
Zeichner-David M., MacDougall M., Davis A., Vides J., Snead M., Slavkin H. (1989). Enamelins and amelogenins share the same amino-terminal sequence. Connect Tissue Res22:123-129.
