Albumin protein is present in developing teeth of several species. Oligomer primers and cRNA probes specific for albumin were designed to perform RT-PCR, and for in situ hybridization, respectively. In situ hybridization failed to reveal albumin expression in any tooth cells, however, albumin PCR products were amplified from tissues adhering to the roots of developing teeth from four-week-old mice. It is concluded that this source is not the primary source of albumin protein found in developing enamel, because of the location and level of expression of albumin mRNA in periodontal tissue.
Chen J., Singh K., Mukherjee BB, Sodek J. (1993). Developmental expression of osteopontin (OPN) mRNA in rat tissues: evidence for a role for OPN in bone formation and resorption . Matrix13:113-123.
2.
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry18:5294-5299.
3.
Couwenhoven RI, Davis C., Snead M. (1989). Mouse ameloblasts do not transcribe the albumin gene. Calcif Tissue Int45:367-371.
4.
Craig AM, Smith JH, Denhardt DT (1989). Osteopontin, a transformation-associated cell adhesion phosphoprotein, is induced by 12-O-tetradecanoylphorbol 13-acetate in mouse epidermis. J Biol Chem264:9682-9689.
5.
Forsman K.Lind L., Backman B., Westermark E., Nolmgren G. (1994). Localization of a gene for autosomal dominate amelogenesis imperfecta (ADAI) to chromosome 4q. Hum Molec Genet3:1621-1625.
6.
George A., Sabsay B., Simonian PAL, Veis A. ( 1993). Characterization of a novel dentin matrix acidic phosphoprotein . J Biol Chem268:12624-12630.
7.
Gibson CW, Golub E., Herold R., Risser M., Ding W., Shimokawa H., et al. (1991). Structure and expression of the bovine amelogenin gene. Biochemistry30:1075-1079.
8.
Gibson CW, Lally E., Herold RC, Decker S., Brinster RL, Sandgren EP (1992). Odontogenic tumors in mice carrying albumin-myc and albumin-ras transgenes. Calcif Tissue Int51:162-167.
9.
Hu J., Isom HC ( 1994). Suppression of albumin enhancer activity by H-ras and AP-1 in hepatocyte cell lines. Molec Cell Biol14:1531-1543.
10.
Hu JM, Camper SA, Tilghman SM, Miller T., Georgoff I., Serra R., et al. (1992). Functional analyses of albumin expression in a series of hepatocyte cell lines and in primary hepatocytes. Cell Growth Differ3:577-588.
11.
Kioussis D., Eiferman F., van de Rijn P., Gorin MB, Ingram RS, Tilghman SM (1981). The evolution of alphafetoprotein and albumin . J Biol Chem256:1960-1967.
12.
Koyama E., Leatherman JL, Shimazu A., Nah HD, Pacifici M. (1995). Syndecan-3, tenascin-C, and the development of cartilaginous skeletal elements and joints in chick limbs. Dev Dynam203:152-162.
13.
Limeback H., Sodek J., Aubin JE (1983). Variation in collagen expression by cloned periodontal ligament cells. J Periodont Res18:242-248.
14.
Limeback H., Sakarya H., Chu W., Mackinnon M. (1989). Serum albumin and its acid hydrolysis peptides dominate preparations of mineral-bound enamel proteins. J Bone Min Res4:235-241.
15.
McCulloch CAG, Bordin S. (1991). Role of fibroblast subpopulations in periodontal physiology and pathology. J Periodont Res26:144-154.
16.
Menanteau J., Gregoire M., Daculsi G., Jans I. ( 1987). In vitro albumin binding on apatite crystals from developing enamel. Bone Mineral3:137-141.
17.
Menanteau J., Dajean S., Laboux O., Aubry J. (1990). Proteins of the mineral compartment of bovine fetal enamel share common antigenic determinants with serum proteins. Calcif Tissue Int47:251-255.
18.
Mukai M., Yoshimine Y., Akamine A., Maeda K. (1993). Bone-like nodules formed in vitro by rat periodontal ligament cells. Cell Tissue Res271:453-460.
19.
Nahon JL (1987). The regulation of albumin and alphafetoprotein gene expression in mammals. Biochimie69:445-459.
20.
Naval J., Calvo M., Laborda J., Dubouch P., Frain M., Sala-Trepat JM, et al. (1992). Expression of mRNAs for alphafetoprotein (AFP) and albumin and incorporation of AFP and docosahexaenoic acid in baboon fetuses. J Biochem111:649-654.
21.
Otsuka K., Pitaru S., Overall CM, Aubin JE, Sodek J. (1988). Biochemical comparison of fibroblast populations from different periodontal tissues: characterization of matrix protein and collagenolytic enzyme synthesis. Biochem Cell Biol66:167-176.
22.
Poliard A., Feldmann G., Bernuau D. (1988). Alphafetoprotein and albumin gene transcripts are detected in distinct cell populations of the brain and kidney of the developing rat. Differentiation39:59-65.
23.
Robinson C., Kirkham J., Brookes SJ, Shore RC (1992). The role of albumin in developing rodent dental enamel: a possible explanation for white spot hypoplasia. J Dent Res71:1270-1274.
24.
Robinson C., Brookes SJ, Kirkham J., Shore RC, Bonass WA (1994). Uptake and metabolism of albumin by rodent incisor enamel in vivo and postmortem: implications for control of mineralization by albumin. Calcif Tissue Int55:467-472.
25.
Sambrook J., Fritsch EF, Maniatis T. (1989). Molecular cloning, a laboratory manual. 2nd ed. Plainview, NY: Cold Spring Harbor Laboratory Press.
26.
Sandgren EP, Quaife CJ, Pinkert CA, Palmiter RD, Brinster RL (1989). Oncogene-induced liver neoplasia in transgenic mice. Oncogene4:715-724.
27.
Sanger F., Nicklen S., Coulson AR (1977). DNA sequencing with chain-terminating inhibitors . Proc Natl Acad Sci USA74:5463-5467.
28.
Somerman MJ, Young MF, Foster RA, Moehring JM, Imm G., Sauk JJ ( 1990). Characteristics of human periodontal ligament cells in vitro. Arch Oral Biol35:241-247.
29.
Somerman MJ, Shroff B., Foster RA, Butler WT, Sauk JJ ( 1992). Mineral-associated adhesion proteins are linked to root formation. Proc Finn Dent Soc88(Suppl 1):451-461.
30.
Southern EM (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Molec Biol98:503-517.
31.
Strawich E., Glimcher MJ (1990). Tooth 'enamelins' identified mainly as serum proteins . Eur J Biochem191:47-56.
32.
Young MF, Bolander ME, Day AA, Ramis CI, Robey PG, Yamada Y., et al. (1986). Osteonectin mRNA: distribution in normal and transformed cells. Nucleic Acids Res14:4483-4497.
33.
Young MF, Kerr JM, Ibaraki K., Heegaard AM, Robey PG (1992). Structure, expression and regulation of the major noncollagenous matrix proteins of bone. Clin Orthop Rel Res281:275-294.
