Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption

Abstract

In this study, the effects of culture supernatants on various activities of the monocyte, as a bone-resorbing cell, were compared between peripheral blood leukocyte (PBL) cultures from patients with periodontal disease and those from subjects with a clinically healthy periodontium. We have reported that normal human monocytes in vitro induce the release of calcium from synthetic hydroxyapatite particles and that the activity is enhanced by supernatants from cultures of stimulated or non-stimulated peripheral blood leukocytes.

Monocytes from both patients and healthy subjects induced the release of calcium from hydroxyapatite particles (HA) to an equal degree. This activity of monocytes from healthy subjects showed a statistically significant increase by addition of supernatants from stimulated or unstimulated cultures of peripheral blood leukocytes from periodontitis patients. This increase was greater than that seen with supernatants from cells of healthy controls. The Nitro Blue Tetrazolium reduction activity and [³H]-thymidine incorporation of monocytes were also increased by addition of the supernatants from leukocyte cultures from either patients or healthy controls, but no significant difference was noted in the increase.

These results suggest that the HA-resorbing activity of monocytes was enhanced by factors from cultured leukocytes. Furthermore, these studies showed that production of these factors by peripheral mononuclear cells from patients with periodontal disease was greater than that seen with cells from normal subjects.

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References

Ash, P. ; Loutit, J.F. ; and Townsend, K.M.S. (1980): Osteoclasts Derived From Haematopoietic Stem Cells, Nature 283: 669-670.

Baehner, R.L. and Nathan, D.G. (1968): Quantitative Nitro-blue Tetrazolium Test in Chronic Granulomatous Disease, New Eng J Med 278: 1971-1976.

Baker, J.J. ; Chan, S.P. ; Socransky, S.S. ; Oppenheim, J.J. ; and Mergenhagen, S.E. (1976): Importance of Actinomyces and Certain Gram-negative Anaerobic Organisms in the Transformation of Lymphocytes from Patients with Periodontal Disease, Infect Immun 13: 1363-1368.

Chambers, T.J. (1981): Resorption of Bone by Mouse Peritoneal Macrophages , J Pathol 135: 295-299.

Evans, R.W. ; Cheung, H.S. ; and McCarty, D.J. (1984): Cultured Human Monocytes and Fibroblasts Solubilize Calcium Phosphate Crystals, Calcif Tissue Int 36: 645-650.

Heersche, J.N.M. (1978): Mechanism of Osteoclastic Bone Resorption ; A New Hypothesis, Calcif Tissue Res 26: 81-84.

Ivanyi, L. and Lehner, T. (1971): Lymphocyte Transformation by Sonicates of Dental Plaque in Human Periodontal Disease, Arch Oral Biol 16: 1117-1121.

Kahn, A.J. and Simmons, D.J. (1975): Investigation of Cell Lineage in Bone Using a Chimera of Chick and Quail Embryonic Tissue, Nature 258: 325-327.

Kahn, A.J. ; Stewart, C.C. ; and Teitelbaum, S.L. (1978): Contact-mediated Bone Resorption by Human Monocytes in vitro, Science 199: 988-990.

10.

Loe, H. and Silness, J. (1963): Periodontal Disease in Pregnancy. I. Prevalence and Severity, Acta Odontol Scand 21: 533-551.

11.

Mackler, B.F. ; Altman, L.C. ; Wahl, S. ; Rosenstreich, D.L. ; Oppenheim, J.J. ; and Mergenhagen, S.E. (1974): Blastogenesis and Lymphokine Synthesis by T and B Lymphocytes from Patients with Periodontal Disease, Infect Immun 10: 844-850.

12.

McArthur, W. ; Yaari, A.M. ; and Shapiro, LM (1980): Bone Solubilization by Mononuclear Cells, Lab Invest 42: 450-456.

13.

Mundy, G.R. ; Altman, A.J. ; Gondek, M.D. ; and Bande-Lin, J.G. (1977): Direct Resorption of Bone by Human Monocytes, Science 196: 1109-1111.

14.

Rifkin, B.R. ; Baker, R.L. ; Somerman, M.J. ; Pointon, S.E. ; Coleman, S.J. ; and Au, W.Y.W. (1980): Osteoid Resorption by Mononuclear Cells in vitro , Cell Tissue Res 210: 493-500.

15.

Russell, A.L. (1956): A System of Classification and Scoring for Prevalence Surveys of Periodontal Disease, J Dent Res 35: 350-359.

16.

Sato, I. ; Fujihashi, H. ; Iwakawa, Y. ; Nakamura, Y. ; Marumoto, Y. ; and Ikeda, K. (1985): Studies on Bone Resorption by Human Monocytes , J Jpn Assoc Peridont 27: 146-152.

17.

Sato, L; Fujihashi, H. ; Iwakawa, Y. ; Nishimoto, M. ; Marumoto, Y. ; and Ikeda, K. (1986): Effect of Culture Supernatants of Peripheral Blood Leukocytes on Bone-Mineral Resorbing Activity by Human Monocytes, J Jpn Assoc Periodont 27: 830-835.

18.

Sato, I. ; Iwakawa, Y. ; Fujihashi, H. ; Tatsumi, J. ; Shi-Moyama, M. ; Marumoto, Y. ; and Ikeda, K. (1987): Study on the Mechanism of Bone-mineral Destruction by Human Monocytes, J Jpn Assoc Periodont 29: 530-537.

19.

Smith, F.N. and Lang, N.P. (1977): Lymphocyte Blastogenesis to Plaque Antigens in Human Periodontal Disease, J Periodont Res 12: 310-317.

20.

Teitelbaum, S.L. ; Malone, J.D. ; and Kahn, A.J. (1981): Glucocorticoid Enhancement of Bone Resorption by Rat Peritoneal Macrophages in vitro, Endocrinology 108: 795-799.

21.

Teitelbaum, S.L. ; Stewart, C.C. ; and Kahn, A.J. (1979): Rodent Peritoneal Macrophages as Bone Resorbing Cells, Calcif Tissue Int 27: 255-261.

22.

Thorsby, E. and Bartlie, A. (1971): A Rapid Method for Preparation of Pure Lymphocyte Suspensions. In: Histocompatibility Testing 1970, P.I. Terasaki , Ed., Copenhagen: Munksgaard, pp. 655-656.

23.

Yam, L.T. ; Li, C.Y. ; and Crosby, W.H. (1971): Cytochemical Identification of Monocytes and Granulocytes , Am J Clin Pathol 55: 283-290.