Cultures of human periodontal ligament fibroblasts (HPLF) and human alveolar bone cells (HABC) were established, maintained, and characterized with respect to alkaline phosphatase (ALPase) activity, a marker enzyme of bone cells. During the proliferative phase, ALPase of HPLF and HABC were not detectable. But at the confluent stage, HPLF synthesized levels of ALPase similar to HABC. Also, ALPase activity of HPLF was markedly stimulated (7.5-fold) by 5 x 10-9 mol/L 1,25 (OH)2-vitamin D3 (1,25 (OH)2-D3). Therefore, since ALPase activity of HPLF resembles that present in osteoblasts, human periodontal ligament fibroblasts may function as "osteoblastic" fibroblasts.
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