Light and Electron Microscopic Studies of Experimentally-Induced Pathologic Changes in the Rat Periodontal Tissue

Abstract

Morphological changes in periodontal tissues following topical application of lipopolysaccharide (LPS) or prostaglandin E₂ (PGE₂) were examined in Wistar rats by light and electron microscopy. Ultrastructural demonstration of acid phosphatase and immunohistochemical localization of lysozyme in these tissues were also studied. The number of osteoclasts along the alveolar bone facing the periodontal ligament was counted.

After topical application of LPS, degeneration and necrosis of the junctional epithelial cells, gingival fibroblasts, and alveolar osteoblasts were observed, as were increased bone resorption by osteoclasts and enhanced phagocytosis of collagen fibrils by fibroblasts. LPS appears to traverse easily through the junctional epithelium into the underlying gingival connective tissues. There it can cause destruction of deeper periodontal tissues by activating alveolar bone resorption and by enhancing the phagocytosis of collagen fibrils. PGE₂ topically applied induced similar morphological changes. Possible participation of PGE₂ in the induction of these LPS-related changes is discussed.

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References

Barka, T. and Anderson, P.J. (1962): Histochemical Methods for Acid Phosphatase Using Hexazonium Pararosanilin as Coupler, J Histochem Cytochem 10:741-753.

Caffesse, R.G. and Nasjleti, C.E. (1976): Enzymatic Penetration through Intact Sulcular Epithelium, J Periodontol 47:391-397.

Daly, C.G. ; Seymour, G.J. ; and Kieser, J.B. (1980): Bacterial Endotoxin: A Role in Chronic Inflammatory Periodontal Disease, J Oral Pathol 9:1-15.

Garant, P.R. (1976): Collagen Resorption by Fibroblasts. A Theory of Fibroblastic Maintenance of the Periodontal Ligament, J Periodontol 7:380-390.

Goodson, J.M. ; McClatchy, K. ; and Revell, C. (1974): Prostaglandin-induced Resorption of the Adult Rat Calvarium, J Dent Res 53:670-677.

Hausmann, E. ; Raisz, L.G. ; and Miller, W.A. (1970): Endotoxin : Stimulation of Bone Resorption in Tissue Culture, Science 158:862-864.

Kajikawa, K. ; Yamaguchi, T. ; Katsuda, S. ; and Miwa, A. (1975): An Improved Electron Stain for Elastic Fibers Using Tannic Acid, J Electron Microscopy 24:287-289.

Karnovsky, M.J. (1965): A Formaldehyde-Glutaraldehyde Fixative of High Osmolarity for Use in Electron Microscopy, J Cell Biol 27:137A-138A.

Kurland, J.I. and Bockman, R. (1978): Prostaglandin E Production by Human Blood Monocytes and Mouse Peritoneal Macrophages, J Exp Med 147:952-957.

10.

Listgarten, M.A. (1975): Similarity of Epithelial Relationships in the Gingiva of Rat and Man, J Periodontol 46:677-680.

11.

McDougall, W.A. (1971): Penetration Pathways of a Topically Applied Foreign Protein into Rat Gingiva, J Periodont Res 6 :89-99.

12.

Miyayama, H. ; Solomon, R. ; Sasaki, M. ; Lin, C.-W. ; and Fishman, W.H. (1975): Demonstration of Lysosomal and Extralysosomal Sites for Acid Phosphatase in Mouse Kidney Tubule Cells with p-Nitrophenylphosphate Lead-salt Technique, J Histochem Cytochem 23:439-451.

13.

Perez-Tamayo, R. (1970): Collagen Resorption in Carrageenin Granulomas. II. Ultrastructure of Collagen Resorption, Lab Invest 22:142-159.

14.

Pettigrew, D.W. ; Wang, H.-M. ; Sodek, J. ; and Brunette, D.M. (1981): Synthesis of Collagenolytic Enzymes and their Inhibitors by Gingival Tissue in vitro. Effect of Endotoxin, J Periodont Res 16:637-645.

15.

Raisz, L.G. ; Nuki, K. ; Alander, C.B. ; and Craig, R.G. (1981): Interactions between Bacterial Endotoxin and Other Stimulators of Bone Resorption in Organ Culture, J Periodont Res 16:1-7.

16.

Ratcliff, G.A. (1966): Permeability of Healthy Gingival Epithelium by Microscopically Observable Particles, J Periodontol 37 :291-293.

17.

Rizzo, A.A. and Mergenhagen, S.E. (1964): Histopathologic Effects of Endotoxin Injected into Rabbit Oral Mucosa, Arch Oral Biol 9:659-670.

18.

Rose, G.G. ; Yajima, T. ; and Mahan, C.J. (1978): Microscopic Assay for the Phagocytic Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro, J Dent Res 57 :1003-1015.

19.

Schwartz, J. ; Stinson, F.L. ; and Parker, R.B. (1972): The Passage of Tritiated Bacterial Endotoxin across Intact Gingival Crevicular Epithelium, J Periodontol 43:270-276.

20.

Tanaka, T. (1984): Transport Pathway and Uptake of Micro-peroxidase in the Junctional Epithelium of Healthy Rat Gingiva, J Periodont Res 19:26-39.

21.

Yajima, T. (1986): Acid Phosphatase Activity in Intracellular Collagen Degradation by Fibroblasts in vitro, Cell Tissue Res 245 :253-260.

22.

Yamasaki, A. ; Nikai, H. ; Niitani, K. ; and Ijuhin, N. (1979): Ultrastructure of the Junctional Epithelium of Germfree Rat Gingiva, J Periodontol 50:641-648.