Molecular cloning of a bovine amelogenin cDNA was accomplished by construction of a cDNA expression library (λgt11 cDNA library) from the bovine ameloblast mRNA and then screening of the library with antibodies to bovine amelogenins. The complete primary structure of an amelogenin was deduced from cloned cDNA. One of the cDNA clones isolated from a bovine ameloblast phage λgt11 library had an 864-base-pair-long insert that encoded a protein with 216 amino acid residues. This cDNA clone appears to represent the complete coding region of amelogenin mRNA, including a putative AUG initiation codon and a signal peptide sequence. The predicted bovine amelogenin sequence has 87% amino acid homology with murine amelogenin.
Get full access to this article
View all access options for this article.
References
1.
Aviv, H. and Leder, P. (1972): Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic-Cellulose, Proc Natl Acad Sci USA69:1408-1412.
2.
Burgess, R.C. and MacLaren, C.M. (1965): Proteins in Developing Bovine Enamel. In: Tooth Enamel. Its Composition, Properties, and Fundamental Structure, M.V. Stack and R.W. Eds., Bristol: John Wright, pp. 74-82.
3.
Eastoe, J.E. (1963): Amino Acid Composition of Proteins from the Oral Tissues - II. The Matrix Proteins in Dentine and Enamel from Developing Human Deciduous Teeth, Arch Oral Biol8 :633-652.
4.
Fincham, A.G.; Belcourt, A.B.; and Termine, J.D. (1981): The Molecular Composition of Bovine Fetal Enamel Matrix. In: The Chemistry and Biology of Mineralized Connective Tissues, A. Veis, Ed., Amsterdam: North Holland/Elsevier , pp. 523-529.
5.
Fincham, A.G.; Belcourt, A.B.; and Termine, J.D. (1982): Changing Patterns of Enamel Matrix Proteins in the Developing Bovine Tooth, Caries Res16:64-71.
6.
Fukae, M. and Shimizu, M. (1983): Amino Acid Sequence of the Main Component of Porcine Enamel Proteins, Jpn J Oral Biol25 (Suppl): 29.
7.
Hughs, R.C. (1973): Glycoproteins as Components of Cellular Membranes , Prog Biophys Molec Biol26:189-268.
8.
Kyte, J. and Doolittle, R.F. (1982): A Simple Method for Displaying the Hydropathic Character of a Protein, J Molec Biol157:105-132.
9.
Messing, J.; Crea, R.; and Seeburg, P.H. (1981): A System for Shotgun DNA Sequencing, Nucl Acid Res9:309-321.
10.
Robinson, C.; Briggs, H.D., Atkinson, P.J.; and Weath-Erell, J.A. (1979): Matrix and Mineral Changes in Developing Enamel, J Dent Res58B:871-880.
11.
Sanger, F.; Nicklen, S.; and Carlson, A.R. (1977): DNA Sequencing with Chain-terminating Inhibitors , Proc Natl Acad Sci USA74:5463-5467.
12.
Sasaki, S. and Shimokawa, H. (1979): Enamel Proteins: Biosynthesis and Chemistry, J Dent Res58B:765-771.
13.
Shimokawa, H. and Sasaki, S. (1978): Study on the Biosynthesis of Bovine Enamel Protein in vitro, J Dent Res57:133-138.
14.
Shimokawa, H.; Wassmer, P.; Sobel, M.E.; and Termine, J.D. (1984): Characterization of Cell-free Translation Products of mRNA from Bovine Ameloblasts by Monoclonal and Polyclonal Antibodies. In: Tooth Enamel IV, R. W. Fearnhead and S. Suga, Eds., Amsterdam: Elsevier, pp. 161-166.
15.
Shimokawa, H.; Sobel, M.E., Sasaki, M.; Termine, J.D.; and Young, M.F. (1987): Heterogeneity of Amelogenin mRNA in the Bovine Tooth Germ, J Biol Chem262:4042-4047.
16.
Snead, M.L.; Lau, E.C.; Zeichner-David, M.; Fincham, A.G.; Woo, S.L.C.; and Slavkin, H.C. (1985): DNA Sequence for Cloned cDNA for Murine Amelogenin Reveals the Amino Acid Sequence for Enamel Specific Protein, Biochem Biophys Res Commun129:812-818.
17.
Takagi, T.; Suzuki, M.; Baba, T.; Minegishi, K.; and Sasaki, S. (1984): Complete Amino Acid Sequence of Amelogenin in Developing Bovine Enamel, Biochem Biophys Res Commun121:592-597.
18.
Termine, J.D.; Belcourt, A.B.; Christner, P.J.; Conn, K.M.; and Nylen, M.U. (1980): Properties of Dissociatively Extracted Fetal Tooth Matrix Proteins. I. Principal Molecular Species in Developing Bovine Enamel, J Biol Chem255:9760-9768.
19.
Young, R.A. and David, R.W. (1983): Efficient Isolation of Genes by Using Antibody Probes, Proc Natl Acad Sci USA80:1194-1198.
20.
Zeichner-David, M.; Vides, J.; Snead, M.; and Slavkin, H. (1984): Enamel Protein Characterization by mRNA Translation in a Cell Free System. In: The Chemistry and Biology of Mineralized Tissue, W. T. Butler, Ed., Birmingham, Alabama: Ebsco Media, Inc. , pp. 264-269.
