Dietary long-chain polyunsaturated fatty acids are known to benefit infant development. After birth, human milk provides arachidonic, eicosapentaenoic, and docosahexaenoic acids to the infant. Endocannabinoids are endogenous lipid mediators derived from the long-chain polyunsaturated fatty acids. Although the roles and the mechanisms of action are not fully understood, previous researchers have suggested that endocannabinoids might play a role in infant feeding behavior.
Research Aims
To assess (i) maternal dietary intake of long-chain polyunsaturated fatty acids and (ii) their relationship to concentrations of fatty acids and derived endocannabinoids in human milk.
Methods
For this exploratory-longitudinal study, participants (N = 24) provided dietary intake data and milk samples. Fatty acids and derived endocannabinoids: Arachidonylethanolamide, arachidonoylglycerol, docosahexaenoyl glycerol, eicosapentaenoyl ethanolamide, and eicosapenaenoyl glycerol were identified in their milk by liquid chromatography-mass spectrometry and correlations to dietary fatty acids were assessed.
Results
Participants were not consuming recommended amounts of docosahexaenoic acid. Significant correlations (p ≤ .05) were only found between dietary docosahexaenoic and eicosapentaenoic acids and the concentrations of these in human milk. Moreover, only dietary docosahexaenoic acid was correlated (p = .031) with its corresponding endocannabinoid, docosahexaenoyl glycerol.
Conclusions
To the best of our knowledge, this may be one of the first studies evaluating relationships between dietary long-chain polyunsaturated fatty acids and multiple endocannabinoids in human milk. Our findings suggest that endocannabinoid concentrations could be modulated by dietary precursors. Future research studies can be designed based on these data to better elucidate the roles of endocannabinoids in human milk for infant health and development.
American Academy of Pediatrics (2012). Breastfeeding and the use of human milk. Pediatrics, 129(3), e827–e841.doi:10.1542/peds.2011-3552
2.
AndreasN. J.KampmannB.Mehring Le-DoareK. (2015). Human breast milk: A review on its composition and bioactivity. Early Human Development, 91(11), 629–635.doi:10.1016/j.earlhumdev.2015.08.01326375355
3.
ArtmannA.PetersenG.HellgrenL.BobergJ.SkonbergC.NellemannC.HansenS. H.HansenH. S. (2008). Influence of dietary fatty acids on endocannabinoid and N-acylethanolamine levels in rat brain, liver and small intestine. Biochimica et Biophysica Acta (BBA)—Molecular and Cell Biology of Lipids, 1781(4), 200–212.doi:10.1016/j.bbalip.2008.01.006
BallardO.MorrowA. L. (2013). Human milk composition: Nutrients and bioactive factors. Pediatric Clinics of North America, 60(1), 49–74.doi:10.1016/j.pcl.2012.10.00223178060
6.
BergerA.CrozierG.BisognoT.CavaliereP.InnisS.Di MarzoV. (2001). Anandamide and diet: Inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines in piglets. Proceedings of the National Academy of Sciences, 98(11), 6402–6406.doi:10.1073/pnas.10111909811353819
7.
BrennaJ. T.VaraminiB.JensenR. G.Diersen-SchadeD. A.BoettcherJ. A.ArterburnL. M. (2007). Docosahexaenoic and arachidonic acid concentrations in human breast milk worldwide. The American Journal of Clinical Nutrition, 85(6), 1457–1464.doi:10.1093/ajcn/85.6.145717556680
8.
CalderP. C. (2013). Omega-3 polyunsaturated fatty acids and inflammatory processes: Nutrition or pharmacology?British Journal of Clinical Pharmacology, 75(3), 645–662.doi:10.1111/j.1365-2125.2012.04374.x22765297
9.
CascioM. G. (2013). PUFA-derived endocannabinoids: An overview. Proceedings of the Nutrition Society, 72(4), 451–459.doi:10.1017/S002966511300341824020830
Di MarzoV. (2008). Endocannabinoids: Synthesis and degradation. InAmaraS. G.BambergE.FleischmannB.GudermannT.HebertS. C.JahnR.LedererW. J.LillR.MiyajimaA.OffermannsS.ZechnerR. (Eds.), Reviews of Physiology Biochemistry and Pharmacology (pp.1–24).
12.
DurhamH. A.WoodJ. T.VadivelS. K.MakriyannisA.Lammi-KeefeC. J. (2013). Detection of the endocannabinoid metabolome in human plasma and breast milk. The FASEB Journal, 27(Suppl. 1), 45–48.
13.
FrideE. (2004). The endocannabinoid-CB(1) receptor system in pre- and postnatal life. European Journal of Pharmacology, 500(1–3), 289–297.doi:10.1016/j.ejphar.2004.07.03315464041
14.
FrideE.GinzburgY.BreuerA.BisognoT.Di MarzoV.MechoulamR. (2001). Critical role of the endogenous cannabinoid system in mouse pup suckling and growth. European Journal of Pharmacology, 419(2-3), 207–214.doi:10.1016/S0014-2999(01)00953-011426843
15.
*GaitánA. V.WoodJ. T.ZhangF.MakriyannisA.Lammi-KeefeC. J. (2018). Endocannabinoid metabolome characterization of transitional and mature human milk. Nutrients, 10(9), 1294.doi:10.3390/nu1009129430213124
16.
HulleyS. B.CummingsS. R.BrownerW. S.GradyD. G.NewmannT. B. (2015). Designing clinical research (4th ed). Koonja Publisher.
17.
InnisS. M. (2014). Impact of maternal diet on human milk composition and neurological development of infants. The American Journal of Clinical Nutrition, 99(3), 734S–741.doi:10.3945/ajcn.113.07259524500153
18.
Institute of Medicine. (2005). Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids (Macronutrients). The National Academies Press.
19.
JensenC. L.MaudeM.AndersonR. E.HeirdW. C. (2000). Effect of docosahexaenoic acid supplementation of lactating women on the fatty acid composition of breast milk lipids and maternal and infant plasma phospholipids. The American Journal of Clinical Nutrition, 71(1 Suppl), 292s–299.doi:10.1093/ajcn/71.1.292s10617985
20.
JensenC. L.VoigtR. G.LlorenteA. M.PetersS. U.PragerT. C.ZouY. L.RozelleJ. C.TurcichM. R.FraleyJ. K.AndersonR. E.HeirdW. C. (2010). Effects of early maternal docosahexaenoic acid intake on neuropsychological status and visual acuity at five years of age of breast-fed term infants. The Journal of Pediatrics, 157(6), 900–905.doi:10.1016/j.jpeds.2010.06.00620655543
21.
JensenC. L.VoigtR. G.PragerT. C.ZouY. L.FraleyJ. K.RozelleJ. C.TurcichM. R.LlorenteA. M.AndersonR. E.HeirdW. C. (2005). Effects of maternal docosahexaenoic acid intake on visual function and neurodevelopment in breastfed term infants. The American Journal of Clinical Nutrition, 82(1), 125–132.doi:10.1093/ajcn/82.1.12516002810
22.
JiaXPaksereshtMWattarNWildgrubeJSontagSAndrewsMSubhanF. BMcCargarLFieldC. JAPrON study team. (2015). Women who take n-3 long-chain polyunsaturated fatty acid supplements during pregnancy and lactation meet the recommended intake. Applied Physiology, Nutrition, and Metabolism, 40(5), 474–481.doi:10.1139/apnm-2014-031325804267
23.
KoletzkoB. (2016). Human milk lipids. Annals of Nutrition and Metabolism, 69(Suppl. 2), 27–40.doi:10.1159/00045281928103608
24.
KoletzkoBLienEAgostoniCBöhlesHCampoyCCetinIDecsiTDudenhausenJ. WDupontCForsythSHoesliIHolzgreveWLapillonneAPutetGSecherN. JSymondsMSzajewskaHWillattsPUauyRWorld Association of Perinatal Medicine Dietary Guidelines Working Group. (2008). The roles of long-chain polyunsaturated fatty acids in pregnancy, lactation and infancy: Review of current knowledge and consensus recommendations. Journal of Perinatal Medicine, 36(1), 5–14.doi:10.1515/JPM.2008.00118184094
25.
LawrenceR. A.LawrenceR. (2015). Breastfeeding: A guide for the medical professional (8th ed). Elsevier.
26.
MatiasI.CartaG.MurruE.PetrosinoS.BanniS.Di MarzoV. (2008). Effect of polyunsaturated fatty acids on endocannabinoid and N-acyl-ethanolamine levels in mouse adipocytes. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1781(1-2), 52–60.doi:10.1016/j.bbalip.2007.11.00118054340
27.
MillerE. M.AielloM. O.FujitaM.HindeK.MilliganL.QuinnE. A. (2013). Field and laboratory methods in human milk research. American Journal of Human Biology, 25(1), 1–11.doi:10.1002/ajhb.2233423109280
28.
Nutrition Coordinating Center. (2017). Nutrition data system for research. University of Minnesota Press.
29.
SherryC. L.OliverJ. S.MarriageB. J. (2015). Docosahexaenoic acid supplementation in lactating women increases breast milk and plasma docosahexaenoic acid concentrations and alters infant omega 6:3 fatty acid ratio. Prostaglandins, Leukotrienes and Essential Fatty Acids, 95, 63–69.doi:10.1016/j.plefa.2015.01.00525701002
30.
SmithS.KevalaK.CunninghamB.RouseC.HuntC. E.KimH. -Y. (2018). N-Docosahexaenoylethanolamine detected in human breast milk. Prostaglandins, Leukotrienes and Essential Fatty Acids, 137, 1–4.doi:10.1016/j.plefa.2018.07.01630293591
31.
U.S. Department of Agriculture, & U.S. Department of Health and Human Services. (2010). 2015 – 2020 Dietary Guidelines for Americans (7th ed.). https://health.gov/dietaryguidelines/2010.
WilliamsJ.WoodJ.PandarinathanL.KaranianD. A.BahrB. A.VourosP.MakriyannisA. (2007). Quantitative method for the profiling of the endocannabinoid metabolome by LC-atmospheric pressure chemical ionization-MS. Analytical Chemistry, 79(15), 5582–5593.doi:10.1021/ac062408617600384
35.
WoodJ. T.DurhamH. A.VadivelS. K.MakriyannisA.Lammi-KeefeC. J. (2013). Postpartum changes in the endocannabinoid metabolome of human breast milk. The FASEB Journal, 27(Suppl. 1), 615–629.
36.
WoodJ. T.WilliamsJ. S.PandarinathanL.JaneroD. R.Lammi-KeefeC. J.MakriyannisA. (2010). Dietary docosahexaenoic acid supplementation alters select physiological endocannabinoid-system metabolites in brain and plasma. Journal of Lipid Research, 51(6), 1416–1423.doi:10.1194/jlr.M00243620071693
37.
WuJ.Gouveia-FigueiraS.DomellöfM.ZivkovicA. M.NordingM. L. (2016). Oxylipins, endocannabinoids, and related compounds in human milk: Levels and effects of storage conditions. Prostaglandins & Other Lipid Mediators, 122, 28–36.doi:10.1016/j.prostaglandins.2015.11.00226656029
