Athletic girdles have been developed to support the groin, hamstring, and quadriceps muscle groups, to act as a muscle conditioner, to keep muscles warm and to fight muscle fatigue. Athletes' comfort perceptions and athletic performance were compared using a prototype girdle, a girdle currently being used by the university football team, and a non-girdle. Six male volunteers, all physically trained football coaches, served as test subjects. A Repeated Measures Latin Square Design was used for the eighteen 20-minute test sessions on a Stainnaster vertical climbing machine. Skin temperature, perceived comfort, athletic performance, and pre- and post-exercise muscle circumference measures were the independent variables. Both girdles maintained a higher skin temperature during exercise and cool down than the non-girdle. The prototype was rated more favorably than the other girdle for several of the comfort descriptors. No significant difference in athletic performance between garment treatments was found.