A silk elastin-like protein (SELP) is an artificial compound with silk fibroin-like and elastin-like tandem repeats. The objective of this study is to evaluate the influence of SELP on the polarization of human monocytoma cell line (THP-1)-derived macrophages. When the macrophages of inflammation-type (M1) were cultured with different concentrations of SELP solution, the secretion of a pro-inflammatory cytokine, tumor necrotizing factor (TNF) -α was significantly suppressed at the higher concentrations. In addition, the secretion of an anti-inflammation cytokine, interleukin (IL)-10, was significantly enhanced from the macrophage of M0-, M1-, and M2-types. By the incubation with soluble SELP, the morphology of M2-type macrophages changed to be of an extended shape. Following incubation with the sponge of SELP, M0-type macrophages secreted IL-10 with time. It is concluded that the SELP itself in solution has an ability to induce the anti-inflammation of M2-type macrophages.
VacantiJPLangerR. Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. The Lancet1999; 354: S32–S34. DOI: 10.1016/s0140-6736(9990247-7)
2.
LeeKSilvaEAMooneyDJ. Growth factor delivery-based tissue engineering: general approaches and a review of recent developments. Journal of The Royal Society Interface2011; 8: 153–170. DOI: 10.1098/rsif.2010.0223
3.
ZiembaAMGilbertRJ. Biomaterials for local, controlled drug delivery to the injured spinal cord. Frontiers in Pharmacology2017; 8: 245. DOI: 10.3389/fphar.2017.00245
4.
JenniferEWinnetteMKaren F, et al.Controlled-release of IGF-I and TGF-β1 in a photopolymerizing hydrogel for cartilage tissue engineering. Journal of Orthopaedic Research2001; 19: 1098–1104. DOI: 10.1016/S0736-0266(01)00054-7
5.
MouriñoVBoccacciniAR. Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds. Journal of The Royal Society Interface2010; 7: 209–227. DOI: 10.1098/rsif.2009.0379
6.
Müller-EhmsenJ. Survival and development of neonatal rat cardiomyocytes transplanted into adult myocardium. Journal of Molecular and Cellular Cardiology2002; 34: 107–116.
7.
SuzukiKMurtuzaBBeauchampJR, et al.Role of Interleukin-1β in acute inflammation and graft death after cell transplantation to the heart. Circulation2004; 110: 219–224. DOI: 10.1161/01.cir.0000138388.55416.06
8.
SeehermanH. Delivery of bone morphogenetic proteins for orthopedic tissue regeneration. Cytokine2005; 16: 329–345.
9.
SternbergK. Current requirements for polymeric biomaterials in otolaryngology. GMS Current Topics in Otorhinolaryngology, Head and Neck Surgery2009; 8. Doc11: 20110310. DOI: 10.3205/cto000063
10.
JulierZParkAJBriquezPS, et al.Promoting tissue regeneration by modulating the immune system. Acta Biomaterialia2017; 53: 13–28. DOI: 10.1016/j.actbio.2017.01.056
11.
ChungLMaestasDRHousseauF, et al.Key players in the immune response to biomaterial scaffolds for regenerative medicine. Advanced Drug Delivery Reviews2017; 114: 184–192. DOI: 10.1016/j.addr.2017.07.006
12.
SkaperSDFacciLGiustiP. Mast cells, glia and neuroinflammation: partners in crime?Immunology2014; 141: 314–327. DOI: 10.1111/imm.12170
13.
EmingSAKriegTDavidsonJM. Inflammation in wound repair: molecular and cellular mechanisms. Journal of Investigative Dermatology2007; 127: 514–525. DOI: 10.1038/sj.jid.5700701
14.
OgleMESegarCESridharS, et al.Monocytes and macrophages in tissue repair: implications for immunoregenerative biomaterial design. Experimental Biology and Medicine2016; 241: 1084–1097. DOI: 10.1177/1535370216650293
15.
StoutRDSuttlesJ. Functional plasticity of macrophages: reversible adaptation to changing microenvironments. Journal of Leukocyte Biology2004; 76: 509–513. DOI: 10.1189/jlb.0504272
16.
MantovaniABiswasSKGaldieroMR, et al.Macrophage plasticity and polarization in tissue repair and remodelling. The Journal of Pathology2013; 229: 176–185. DOI: 10.1002/path.4133
17.
MantovaniASicaASozzaniS, et al.The chemokine system in diverse forms of macrophage activation and polarization. Trends in Immunology2004; 25: 677–686. DOI: 10.1016/j.it.2004.09.015
18.
FlemingBDMosserDM. Regulatory macrophages: setting the threshold for therapy. European Journal of Immunology2011; 41: 2498–2502. DOI: 10.1002/eji.201141717
MosserDMEdwardsJP. Exploring the full spectrum of macrophage activation. Nature Reviews Immunology2008; 8: 958–969. DOI: 10.1038/nri2448
21.
CappelloJCrissmanJDormanM, et al.Genetic engineering of structural protein polymers. Biotechnology Progress1990; 6: 198–202. DOI: 10.1021/bp00003a006
22.
CappelloJCrissmanJWCrissmanM, et al.In-situ self-assembling protein polymer gel systems for administration, delivery, and release of drugs. Journal of Controlled Release1998; 53: 105–117. DOI: 10.1016/S0168-3659(97)00243-5
23.
HwangDMoolchandaniVDanduR, et al.Influence of polymer structure and biodegradation on DNA release from silk–elastinlike protein polymer hydrogels. International Journal of Pharmaceutics2009; 368: 215–219. DOI: 10.1016/j.ijpharm.2008.10.021
24.
GustafsonJGreishKFrandsenJ, et al.Silk-elastinlike recombinant polymers for gene therapy of head and neck cancer: from molecular definition to controlled gene expression. Journal of Controlled Release2009; 140: 256–261: 20090524. DOI: 10.1016/j.jconrel.2009.05.022
25.
SatoshiSYasuhikoT. An artificial silk-elastin-like protein suppresses cells adhesion without apoptosis. Journal of Biotechnology and Biomaterials2012; 2. DOI: 10.4172/2155-952X.1000139
26.
OzakiCSomamotoSKawabataS, et al.Effect of an artificial silk elastin-like protein on the migration and collagen production of mouse fibroblasts. Journal of Biomaterials Science, Polymer Edition2014; 25: 1266–1277. DOI: 10.1080/09205063.2014.926580
27.
MegeedZCappelloJGhandehariH. Controlled release of plasmid DNA from a genetically engineered silk-elastinlike hydrogel. Pharmaceutical Research2002; 19: 954–959. DOI: 10.1023/a:1016406120288
28.
HaiderMCappelloJGhandehariH, et al.In vitro chondrogenesis of mesenchymal stem cells in recombinant silk-elastinlike hydrogels. Pharmaceutical Research2008; 25: 692–699: 20070403. DOI: 10.1007/s11095-007-9282-8
29.
UnzaiTWashisakaTTabataY. An artificial silk elastin-like protein modifies the polarization of macrophages. ACS Applied Bio Materials2022; 5: 5657–5664: 20221129, DOI:10.1021/acsabm.2c00701
30.
KawabataSKawaiKSomamotoS, et al.The development of a novel wound healing material, silk-elastin sponge. Journal of Biomaterials Science, Polymer Edition2017; 28: 2143–2153: 20170928. DOI: 10.1080/09205063.2017.1382829
31.
RasbandWS. ImageJ. Bethesda, MD, USA: U.S. National Institutes of Health, 2011.
32.
SchindelinJArganda-CarrerasIFriseE, et al.Fiji: an open-source platform for biological-image analysis. Nature Methods2012; 9: 676–682. DOI: 10.1038/nmeth.2019
33.
AbramoffMMagalhãesPRamSJ. Image processing with imageJ. Biophotonics International2003; 11: 36–42.
34.
SchneiderCARasbandWSEliceiriKW. NIH Image to ImageJ: 25 years of image analysis. Nature Methods2012; 9: 671–675. DOI: 10.1038/nmeth.2089
35.
SchwendyMUngerREBonnM, et al.Automated cell segmentation in FIJI using the DRAQ5 nuclear dye. BMC Bioinformatics2019; 20: 39. DOI: 10.1186/s12859-019-2602-2
36.
MurataYJoJITabataY. Preparation of cationized gelatin nanospheres incorporating molecular beacon to visualize cell apoptosis. Scientific Reports2018; 8: 14839. DOI: 10.1038/s41598-018-33231-2
37.
RyusukeTYoichiSYukioF, et al.Preparation of fibrin hydrogels to promote the recruitment of anti-inflammatory macrophages. Acta Biomaterialia2019; 89: 152–165. DOI: 10.1016/j.actbio.2019.03.011
38.
LiB. Curcumin attenuates titanium particle-induced inflammation by regulating macrophage polarization In Vitro and In Vivo. Frontiers in Immunology2017; 8. DOI: 10.3389/fimmu.2017.00055
39.
ZhangFWangHWangX, et al.TGF-β induces M2-like macrophage polarization via SNAIL-mediated suppression of a pro-inflammatory phenotype. Oncotarget2016; 7: 52294–52306. DOI: 10.18632/oncotarget.10561
40.
TsaiYCTsengJTWangCY, et al.Medroxyprogesterone acetate drives M2 macrophage differentiation toward a phenotype of decidual macrophage. Molecular and Cellular Endocrinology2017; 452: 74–83. DOI: 10.1016/j.mce.2017.05.015
41.
NomuraTTeradaNKimWJ, et al.Interleukin-13 induces thymus and activation-regulated chemokine (CCL17) in human peripheral blood mononuclear cells. Cytokine2002; 20: 49–55. DOI: 10.1006/cyto.2002.1979
42.
XiaXXXuQHuX, et al.Tunable self-assembly of genetically engineered silk-elastin-like protein polymers. Biomacromolecules2011; 12: 3844–3850: 20110930. DOI: 10.1021/bm201165h
43.
XiaXXWangMLinY, et al.Hydrophobic drug-triggered self-assembly of nanoparticles from silk-elastin-like protein polymers for drug delivery. Biomacromolecules2014; 15: 908–914. DOI: 10.1021/bm4017594
44.
LiPHaoZWuJ, et al.Comparative proteomic analysis of polarized human THP-1 and mouse RAW264.7 macrophages. Frontiers in Immunology2021; 12: 700009. 20210629. DOI: 10.3389/fimmu.2021.700009
45.
PanilaitisBAltmanGHChenJ, et al.Macrophage responses to silk. Biomaterials2003; 24: 3079–3085. DOI: 10.1016/S0142-9612(03)00158-3
46.
RoySSharmaAGhoshS. Macrophage polarization profiling on native and regenerated silk biomaterials. ACS Biomaterials Science and Engineering2022; 8: 659–671. DOI: 10.1021/acsbiomaterials.1c01432
47.
LiuHWiseSGRnjak-KovacinaJ, et al.Biocompatibility of silk-tropoelastin protein polymers. Biomaterials2014; 35: 5138–5147. DOI: 10.1016/j.biomaterials.2014.03.024
48.
ChanAHPFilipeECTanRP, et al.Altered processing enhances the efficacy of small-diameter silk fibroin vascular grafts. Scientific Reports2019; 9: 1125–17461. DOI: 10.1038/s41598-019-53972-y
49.
KamisatoSUemuraYTakamiN, et al.Involvement of intracellular cyclic GMP and cyclic GMP-dependent protein kinase in alpha-elastin-induced macrophage chemotaxis. Journal of Biochemistry1997; 121: 862–867. DOI: 10.1093/oxfordjournals.jbchem.a021666
50.
DaleMAXiongWCarsonJS, et al.Elastin-derived peptides promote abdominal aortic aneurysm formation by modulating M1/M2 macrophage polarization. Journal of Immunology2016; 196: 4536–4543. DOI: 10.4049/jimmunol.1502454
51.
SeniorRMGriffinGLMechamRP. Chemotactic activity of elastin-derived peptides. Journal of Clinical Investigation1980; 66: 859–862. DOI: 10.1172/jci109926
52.
NénanSPlanquoisJMBernaP, et al.Analysis of the inflammatory response induced by rhMMP-12 catalytic domain instilled in mouse airways. International Immunopharmacology2005; 5: 511–524. DOI: 10.1016/j.intimp.2004.10.011
53.
DiekjiirgenDAstashkinaAGraingerDW, et al.Cultured primary macrophage activation by lipopolysaccharide depends on adsorbed protein composition and substrate surface chemistry. Journal of Biomaterials Science, Polymer Edition2012; 23: 1231–1254. DOI: 10.1163/092050611x580382.20120508.
54.
WillenborgSLucasTvan LooG, et al.CCR2 recruits an inflammatory macrophage subpopulation critical for angiogenesis in tissue repair. Blood2012; 120: 613–625. DOI: 10.1182/blood-2012-01-403386
55.
LiSLiBJiangH, et al.Macrophage depletion impairs corneal wound healing after autologous transplantation in mice. PLOS ONE2013; 8: e61799. DOI: 10.1371/journal.pone.0061799
56.
LeeSSzilagyiEChenL, et al.Activated mesenchymal stem cells increase wound tensile strength in aged mouse model via macrophages. Journal of Surgical Research2013; 181: 20–24. DOI: 10.1016/j.jss.2012.05.040
57.
LeibovichSJRossR. The role of the macrophage in wound repair. a study with hydrocortisone and antimacrophage serum. The American Journal of Pathology1975; 78: 71–100.
58.
PollardJW. Trophic macrophages in development and disease. Nature Reviews Immunology2009; 9: 259–270. DOI: 10.1038/nri2528
TajimaSWachiHUemuraY, et al.Modulation by elastin peptide VGVAPG of cell proliferation and elastin expression in human skin fibroblasts. Archives of Dermatological Research1997; 289: 489–492. DOI: 10.1007/s004030050227
61.
BloodCHSasseJBrodtP, et al.Identification of a tumor cell receptor for VGVAPG, an elastin-derived chemotactic peptide. The Journal of Cell Biology1988; 107: 1987–1993. DOI: 10.1083/jcb.107.5.1987
62.
SeniorRMGriffinGLMechamRP, et al.Val-Gly-Val-Ala-Pro-Gly, a repeating peptide in elastin, is chemotactic for fibroblasts and monocytes. Journal of Cell Biology1984; 99: 870–874. DOI: 10.1083/jcb.99.3.870
63.
LongMMKingVJPrasadKU, et al.Chemotaxis of fibroblasts toward nonapeptide of elastin. Biochimica et Biophysica Acta1988; 968: 300–311. DOI: 10.1016/0167-4889(88)90021-3
64.
WrennDSGriffinGLSeniorRM, et al.Characterization of biologically active domains on elastin: identification of a monoclonal antibody to a cell recognition site. Biochemistry1986; 25: 5172–5176. DOI: 10.1021/bi00366a028
65.
JacobMPFülöpTForisG, et al.Effect of elastin peptides on ion fluxes in mononuclear cells, fibroblasts, and smooth muscle cells. Proceedings of the National Academy of Sciences1987; 84: 995–999. DOI: 10.1073/pnas.84.4.995
66.
SekiyaKOkudaH. Inhibitory action of soluble elastin on thromboxane B2 formation in blood platelets. Biochimica et Biophysica Acta1984; 797: 348–353. DOI: 10.1016/0304-4165(84)90256-3
67.
MauricePBlaiseSGayralS, et al.Elastin fragmentation and atherosclerosis progression: the elastokine concept. Trends in Cardiovascular Medicine2013; 23: 211–221: 20130402. DOI: 10.1016/j.tcm.2012.12.004
68.
DucaLLambertEDebretR, et al.Elastin peptides activate extracellular signal-regulated kinase 1/2 via a ras-independent mechanism requiring both p110γ/Raf-1 and protein kinase A/B-Raf signaling in human skin fibroblasts. Molecular Pharmacology2005; 67: 1315. DOI: 10.1124/mol.104.002725
69.
MitchellMDLairdREBrownRD, et al.IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. The American Journal of Physiology Heart and Circulatory Physiology2007; 292: H1139–H1147: 20061103. DOI: 10.1152/ajpheart.00881.2005
70.
GotohYTsukadaMMinouraN. Effect of the chemical modification of the arginyl residue in Bombyx mori silk fibroin on the attachment and growth of fibroblast cells. Journal of Biomedical Materials Research1998; 39: 351–357. DOI: 10.1002/(SICI)1097-4636(19980305)39:3<351
71.
GotohYTsukadaMMinouraN, et al.Synthesis of poly(ethylene glycol)-silk fibroin conjugates and surface interaction between L-929 cells and the conjugates. Biomaterials1997; 18: 267–271. DOI: 10.1016/s0142-9612(96)00137-8
72.
MinouraNAibaSGotohY, et al.Attachment and growth of cultured fibroblast cells on silk protein matrices. Journal of Biomaterials Science, Polymer Edition1995; 29: 1215–1221. DOI: 10.1002/jbm.820291008
73.
GuptaPKumarMBhardwajN, et al.Mimicking form and function of native small diameter vascular conduits using mulberry and non-mulberry patterned silk films. ACS Applied Materials and Interfaces2016; 8: 15874–15888. DOI: 10.1021/acsami.6b00783
74.
ChambreLMartín-MoldesZParkerRN, et al.Bioengineered elastin- and silk-biomaterials for drug and gene delivery. Advanced Drug Delivery Reviews2020; 160: 186–198, DOI: 10.1016/j.addr.2020.10.008
