Restricted accessResearch articleFirst published online 2023-7
Bone marrow mesenchymal stem cells response on collagen/hyaluronan/chondroitin scaffold enriched with gentamicin -loaded gelatin microparticles for skin tissue engineering
The repair and functional reconstruction of large skin defects caused by burn remains an intractable clinical problem. Collagen type I (ColI) was extracted from carp scales and confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ultraviolet adsorption spectra and automatic amino acid analyzer. Then the scaffolds containing the purified ColI, hyaluronic acid (HA) and chondroitin sulfate (CS) were constructed and examined. The results showed that the scaffold (ColI:CS:HA=9:1:1) had larger pore diameter, porosity, water absorption, degradation rate and tensile strength. gentamycin sulphate (GS) - gelatin microspheres (GMSs) were prepared by emulsion cross-linking method. The drug release study of the ColI-CS-HA-GS/GMSs scaffold with antibacterial property showed a prolonged, continuous, and sustained release of GS. The bone marrow mesenchymal stem cells (BMSCs) were extracted from rat and inoculated into the ColI-HA-CS-GS/GMSs scaffold. The results performed that the scaffold could accelerate proliferation of the BMSCs and wound healing.
YangGSeokJKKangHC, et al.Skin Barrier Abnormalities and Immune Dysfunction in Atopic Dermatitis. Int J Mol Sci2020; 21(8): 2867.
2.
ChambersESVukmanovic-StejicM. Skin barrier immunity and ageing. Immunology2020; 160(2): 116–125.
3.
GudjonssonJEKabashimaKEyerichK. Mechanisms of skin autoimmunity: Cellular and soluble immune components of the skin. J Allergy Clin Immunol2020; 146(1): 8–16.
4.
DąbrowskaAKSpanoFDerlerS, et al.The relationship between skin function, barrier properties, and body-dependent factors. Skin Res Technol2018; 24(2): 165–174.
5.
YaoMHZhangJNGaoF, et al.New BMSC-Laden Gelatin Hydrogel Formed in Situ by Dual-Enzymatic Cross-Linking Accelerates Dermal Wound Healing. ACS Omega2019; 4(5): 8334–8340.
6.
ZengRXTangQHeJY, et al.Experimental study on repairing skin defect by tissue-engineered skin substitute compositely constructed by adipose-derived stem cells and fibrin gel. Eur Rev Med Pharmacol Sci2017; 21(3 Suppl): 1–5.
7.
SorgHTilkornDJHagerS, et al.Skin Wound Healing: An Update on the Current Knowledge and Concepts. Eur Surg Res2017; 58(1–2): 81–94.
8.
QiYDongZChuH, et al.Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice. Burns2019; 45(7): 1685–1694.
9.
TomoukTMohanATAziziA, et al.Donor site morbidity in DIEP free flap breast reconstructions: A comparison of unilateral, bilateral, and bipedicled surgical procedure types. J Plast Reconstr Aesthet Surg2017; 70(11): 1505–1513.
10.
LeonardiDOberdoerferDFernandesMC, et al.Mesenchymal stem cells combined with an artificial dermal substitute improve repair in full-thickness skin wounds. Burns2012; 38(8): 1143–1150.
11.
PengWLiDDaiKL, et al.Recent progress of collagen, chitosan, alginate and other hydrogels in skin repair and wound dressing applications. Int J Biol Macromol2022; 208: 400–408.
12.
WangCWangJZengL, et al.Fabrication of Electrospun Polymer Nanofibers with Diverse Morphologies. Molecules (Basel, Switzerland)2019; 24(5): 834.
13.
FarokhiMMottaghitalabFFatahiY, et al.Overview of Silk Fibroin Use in Wound Dressings. Trends Biotechnol2018; 36(9): 907–922.
14.
QiaoZLvXHeS, et al.A mussel-inspired supramolecular hydrogel with robust tissue anchor for rapid hemostasis of arterial and visceral bleedings. Bioact Mater2021; 6(9): 2829–2840.
15.
GeBSWangHNLiJ, et al.Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings. Mar Drugs2020; 18(4): 178.
RanaMMRahmanMSUllahMA, et al.Amnion and collagen-based blended hydrogel improves burn healing efficacy on a rat skin wound model in the presence of wound dressing biomembrane. Biomed Mater Eng2020; 31(1): 1–17.
18.
Burn and Trauma Branch of Chinese Geriatrics SocietyLyuG. [National experts consensus on clinical application of collagen-based wound biomaterials (2018 version)]. Zhonghua Shao Shang Za Zhi2018; 34(11): 766–769.
19.
Muñoz-GonzálezPULona-RamosMCGutiérrez-VerdínLD, et al.Gel dressing based on type I collagen modified with oligourethane and silica for skin wound healing. Biomed Mater2022; 17(4).
20.
N AmirrahIMohd Razip WeeMFTabataY, et al.Antibacterial-Integrated Collagen Wound Dressing for Diabetes-Related Foot Ulcers: An Evidence-Based Review of Clinical Studies. Polymers2020; 12(9):2168.
21.
Gil-CifuentesLJiménezRAFontanillaMR. Evaluation of collagen type I scaffolds including gelatin-collagen microparticles and Aloe vera in a model of full-thickness skin wound. Drug Deliv Transl Res2019; 9(1): 25–36.
22.
NeumanMGNanauRMOruña-SanchezL, et al.Hyaluronic acid and wound healing. J Pharm Pharm Sci2015; 18(1): 53–60.
23.
VanderAHintzeVScharnweberD, et al.Growth promoting substrates for human dermal fibroblasts provided by artificial extracellular matrices composed of collagen I and sulfated glycosaminoglycans. Biomaterials2011; 32(34): 38–46.
24.
GuoRChenPZMoYF, et al.Synthesis, characterization of dextran hydrogels and their in vitro release of gentamycin sulphate. J Appl Biomater Funct Mater2015; 13(3): 228–233.
25.
TangPSongPPengZ, et al.Chondrocyte-laden GelMA hydrogel combined with 3D printed PLA scaffolds for auricle regeneration. Mater Sci Eng C Mater Biol Appl2021; 130: 112423.
26.
TripathiDRastogiKTyagiP, et al.Comparative Analysis of Collagen and Chitosan-based Dressing for Haemostatic and Wound Healing Application. AAPS PharmSciTech2021; 22(3): 76.
27.
CuiBZhangCGanB, et al.Collagen-tussah silk fibroin hybrid scaffolds loaded with bone mesenchymal stem cells promote skin wound repair in rats. Mater Sci Eng C Mater Biol Appl2020; 109: 110611.
28.
BeghettoVGattoVConcaS, et al.Polyamidoamide Dendrimers and Cross-Linking Agents for Stabilized Bioenzymatic Resistant Metal-Free Bovine Collagen. Molecules2019; 24(19): 3611.
29.
ZhaoWHChiCFZhaoYQ, et al.Preparation, Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Swim Bladders of Miiuy Croaker (Miichthys miiuy). Mar Drugs2018; 16(5): 161.
30.
HeXZhangJWangS, et al.Cloning, expression, purification, and biochemical characterization of CpxR protein from pectobacterium carotovorum. Biotechnol Appl Biochem2022; 69(3): 898–905.
31.
FanBQZhangYJHeY, et al.Adaptive monostable stochastic resonance for processing UV absorption spectrum of nitric oxide. Opt Express2020; 28(7): 9811–9822.
32.
ShahzadRKhanALWaqasM, et al.Metabolic and proteomic alteration in phytohormone-producing endophytic Bacillus amyloliquefaciens RWL-1 during methanol utilization. Metabolomics2019; 15(2): 16.
33.
NiiTTabataY. Immunosuppressive mesenchymal stem cells aggregates incorporating hydrogel microspheres promote an in vitro invasion of cancer cells. Regen Ther2021; 18: 516–522.
34.
LiXXHeJH. Bubble Electrospinning with an Auxiliary Electrode and an Auxiliary Air Flow. Recent Pat Nanotechnol2020; 14(1): 42–45.
35.
Al-FarhanAHAsiriKARajuM, et al.In In Vitro Antimicrobial Activity of Medicinal Plant Rumex Nervosus Against Selected Oral Pathogens. J Pharm Bioallied Sci2022; 14(Suppl 1): S753–S756.
36.
HeXLRenYWMengWL, et al.Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655. AMB Expr2020; 10: 172.
37.
HeLHeTWXingJH, et al.Bone marrow mesenchymal stem cell-derived exosomes protect cartilage damage and relieve knee osteoarthritis pain in a rat model of osteoarthritis. Stem Cell Res Ther2020; 11(1): 276.
38.
GrossetAALoayza-VegaKAdam-GrangerÉ, et al.Hematoxylin and Eosin Counterstaining Protocol for Immunohistochemistry Interpretation and Diagnosis. Appl Immunohistochem Mol Morphol2019; 27(7): 558–563.
39.
WallbergFTenevTMeierP. Analysis of Apoptosis and Necroptosis by Fluorescence-Activated Cell Sorting. Cold Spring Harb Protoc2016; 2016(4), pdb.prot087387.
40.
WangLLiSLuoH, et al.PCSK9 promotes the progression and metastasis of colon cancer cells through regulation of EMT and PI3K/AKT signaling in tumor cells and phenotypic polarization of macrophages. J Exp Clin Cancer Res2022; 41(1): 303.
41.
SoysalEUlutaşFTepeliE, et al.IL-23R gene polymorphisms in rheumatoid arthritis. Rheumatol Int2022; 42(3): 555–562.
42.
HeoJKohRHShimW, et al.Riboflavin-induced photo-crosslinking of collagen hydrogel and its application in meniscus tissue engineering. Drug Deliv Transl Res2016; 6: 148–158.
43.
RenSKWangCXGuoL, et al.Preparation and Sustained-Release Performance of PLGA Microcapsule Carrier System. Nanomaterials (Basel)2021; 11(7): 1758.
44.
AmirsadeghiAJafariAEggermontLJ, et al.Vascularization strategies for skin tissue engineering. Biomater Sci2020; 8(15): 4073–4094.
45.
ChaudhariAAVigKBaganiziDR, et al.Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review. Int J Mol Sci2016; 17(12): 1974.
46.
TheocharisADSkandalisSSGialeliC, et al.Extracellular matrix structure. Adv Drug Deliv Rev2016; 97: 4–27.
47.
LiuYMaDWangY, et al.A comparative study of the properties and self-aggregation behavior of collagens from the scales and skin of grass carp (Ctenopharyngodon idella)Int J Biol Macromol2018; 106: 516–522.
48.
QiZ. Preparation method of fish skin/scale collagen peptide. 2022; A: ZA2022006031.
49.
ChenSYiRChenH, et al.Large-scale preparation of type I collagen peptide from fish scale. 2014; A: CN104140992.
50.
GargTRathGGoyalAK. Biomaterials-based nanofiber scaffold: targeted and controlled carrier for cell and drug delivery. J Drug Target2015; 23(3): 202–221.
51.
DongZXMengXYYangW, et al.Progress of gelatin-based microspheres (GMSs) as delivery vehicles of drug and cell. Mater Sci Eng C Mater Biol Appl2021; 122: 111949.
52.
IwakiMNoguchiNNakaminamiH, et al.Antimicrobial activity and frequency of spontaneous gentamicin-resistant mutants in bacteria related skin infections. Yakugaku Zasshi2011; 131(11): 1653–1659.
53.
ZhengJZLinYSTangFQ, et al.Promotive Role of CircATRNL1 on Chondrogenic Differentiation of BMSCs Mediated by miR-338-3p. Arch Med Res2021; 52(5): 514–522.
Supplementary Material
Please find the following supplemental material available below.
For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.
For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.
