In this research, an experiment was conducted on how the surface wettability and biocompatibility of staplers’ titanium nails impact the healing of gastrointestinal tissue. Firstly, the bionic hexagonal structure was prepared on the surface of Ti metal by laser processing technology, and the laser textured titanium samples were observed by scanning electron microscope. Then, the liquid-solid contact angle-measuring instrument was used to characterize the wettability of titanium samples. Finally, cells were cultured on the surface of different titanium samples, CCK8 assay and qRT-PCR were carried out to investigate cell adhesion and collagen secretion on the surface of different samples. The results showed that the bionic hexagonal surface increased the surface roughness, reduced the liquid-solid contact angle, and promoted the adhesion and collagen secretion of fibroblasts. The increased wettability provided a better growth environment for cell growth. Microtexture is an important factor affecting the behavior of cells and its size parameters regulate cell gene expression, which is worthy of further study.
KanoMHanariNGunjiH, et al.Is “functional end-to-end anastomosis” really functional? A review of the literature on stapled anastomosis using linear staplers. Surg Today2017; 47(1): 1–7.
2.
MarkarSRPennaMVenkat-RamenV, et al.Influence of circular stapler diameter on postoperative stenosis after laparoscopic gastrojejunal anastomosis in morbid obesity. Surg Obes Relat Dis2012; 8(2): 230–235.
3.
WangXPeiXLiX, et al.Predictive calue of anastomotic blood supply for anastomotic stricture after esophagectomy in esophageal cancer. Dig Dis Sci2019; 64(11): 3307–3313.
4.
QuSXiaJYanJ, et al.In vivo and in vitro assessment of the biocompatibility and degradation of high-purity Mg anastomotic staples. J Biomater Appl2017; 31(8): 1203–1214.
5.
SparreboomCLWuZ-QJiJ-F, et al.Integrated approach to colorectal anastomotic leakage: Communication, infection and healing disturbances. World J Gastroenterol2016; 22(32): 7226–7235.
6.
AlverdyJCSchardeyHM. Anastomotic leak: toward an understanding of its root causes. J Gastrointest Surg2021; 25(11): 2966–2975.
7.
MendonçaGMendonçaDBSAragãoFJL, et al.Advancing dental implant surface technology – From micron- to nanotopography. Biomaterials2008; 29(28): 3822–3835.
8.
YuWQJiangXQZhangFQ, et al.The effect of anatase TiO2 nanotube layers on MC3T3-E1 preosteoblast adhesion, proliferation, and differentiation. J Biomed Mater Res A2010; 94A(4): 1012–1022.
9.
BettingerCJLangerRJeffreyT, et al.Engineering substrate topography at the micro- and nanoscale to control cell function. Angew Chem Int Edition2010; 48(30): 5406–5415.
10.
WardBCWebsterTJ. The effect of nanotopography on calcium and phosphorus deposition on metallic materials in vitro. Biomater2006; 27(16): 3064–3074.
11.
VandrovcováMBačákováL. Adhesion, growth and differentiation of osteoblasts on surface-modified materials developed for bone implants. Physiol Res2011; 60(3): 403–417.
12.
GuoHYLiBFengXQ. Stability of Cassie-Baxter wetting states on microstructured surfaces. Phys Rev E2016; 94(4): 042801.
13.
AmaniHArzaghiHBayandoriM, et al.Controlling cell behavior through the design of biomaterial surfaces: a focus on surface modification techniques. Adv Mater Interfaces2019; 6(13): 1900572.
14.
SchaeskeJFadeevaESchlie-WolterS, et al.Cell type-specific adhesion and migration on laser-structured opaque surfaces. Int J Mol Sci2020; 21(22): 8442.
15.
FanFWuYXinH, et al.Surface characteristics and biocompatibility of ultrafine-grain Ti after sandblasting and acid etching for dental implants. ACS Biomater Sci Eng2019; 5(10): 5107–5115.
16.
OliveiraD.P.PalmieriACarinciF, et al.Gene expression of human osteoblasts cells on chemically treated surfaces of Ti–6Al–4V–ELI. Mat Sci Eng C Mater Biol Appl2015; 51: 248–355.
17.
CaiSWuCYangW, et al.Recent advance in surface modification for regulating cell adhesion and behaviors. Nanotechnol Rev2020; 9(1): 971–989.
18.
FadeevaEDeiwickAChichkovB, et al.Impact of laser-structured biomaterial interfaces on guided cell responses. Interf Focus2014; 4(1): 20130048.
19.
BabaliariEKavatzikidouPMitrakiA, et al.Combined effect of shear stress and laser-patterned topography on schwann cell outgrowth: synergistic or antagonistic?Biomater Sci2021; 9: 1334–1344.
20.
SchnellGStaehlkeSDuenowU, et al.Femtosecond laser nano/micro textured Ti6Al4V surfaces—Effect on wetting and MG-63 cell adhesion. Materials2019; 12(13): 2210.
21.
Martínez-CalderonMManso-SilvánMRodríguezA, et al.Surface micro- and nano-texturing of stainless steel by femtosecond laser for the control of cell migration. Sci Rep2016; 6: 36296.
22.
GaoPYMaYDSunWW等.Microstructure and properties of Al2O3–ZrO2–TiO2 composite coatings prepared by plasma spraying. Rare Metals, 2020; 40: 1825–1834.
23.
HarrisLGPattersonLMBaconC, et al.Assessment of the cytocompatibility of different coated titanium surfaces to fibroblasts and osteoblasts. J Biomed Mater Res A2005; 73a(1): 12–20.
24.
WuSLiuXHuT, et al.A biomimetic hierarchical scaffold: Natural growth of nanotitanates on three-dimensional microporous Ti-based metals. Nano Lett2008; 8(11): 3803–3808.
25.
JianfeiLiangShanshan, et al.Osteogenic activity of titanium surfaces with hierarchical micro-/nano-structures obtained by hydrofluoric acid treatment. Int J Nanomed2017; 12: 1317–1328.
26.
PopatKEltgrothMLatempaT, et al.Titania nanotubes: a novel platform for drug-eluting coatings for medical implants?Small2010; 3(11): 1878–1881.
27.
HuangQLiuXElkhoolyT A, et al.A novel titania/calcium silicate hydrate hierarchical coating on titanium. Colloids Surf B Biointerfaces, 2015; 134: 169–177.
28.
SamuelJJunMBGOzdoganlarB, et al.Micro/meso-scale mechanical machining 2020: a two-decade state-of-the-field review. J Manuf Sci Eng2020; 142(11): 1–35.
29.
JiangHWangCRenZ, et al.Influence of cutting velocity on gradient microstructure of machined surface during turning of high-strength alloy steel. Mater Sci Eng A, 2021; 819: 141354.
30.
ZhangSLinXChenJ, et al.Influence of processing parameter on the microstructure and forming characterizations of Ti-6AI-4V titanium alloy after laser rapid forming processing. Rare Metal Mater Eng2007; 36(10): 1839–1843.
31.
WangZuyongZhou, et al.Reliable laser fabrication: the quest for responsive biomaterials surface. J Mater Chem B Mater Biol Med2018; 6: 3621–3631.
32.
SunGTongZFangX, et al.Effect of scanning speeds on microstructure and wear behavior of laser-processed NiCr-Cr3C2-MoS2-CeO2 on 38CrMoAl steel. Opt Laser Technol2016; 77: JOLTD1500370.
33.
LiuSYangW SShiX, et al.Influence of laser process parameters on the densification, microstructure, and mechanical properties of a selective laser melted AZ61 magnesium alloy. J Alloys Compounds2019; 808: 151160.
34.
LiSCuiZZhangW, et al.Biocompatibility of micro/nanostructures nitinol surface via nanosecond laser circularly scanning. Mater Lett2019; 255: 126591.
35.
YuZ YYangGZhangW, et al.Investigating the effect of picosecond laser texturing on microstructure and biofunctionalization of titanium alloy. J Mater Process Technol2018; 255: 129–136.
36.
ZhangJGuanYLinW, et al.Enhanced mechanical properties and biocompatibility of Mg-Gd-Ca alloy by laser surface processing. Surf Coat Technol2019; 362: 176–184.
37.
HindyAFa RahmandFTabatabaeiFS. In vitro biological outcome of laser application for modification or processing of titanium dental implants. Lasers Med Sci2017; 32(5): 1197–1206.
38.
XuNFuJZhaoL, et al.Biofunctional elements incorporated nano/microstructured coatings on titanium implants with enhanced osteogenic and antibacterial performance. Adv Healthc Mater, 2020; 9(23): 2000681.
39.
HermanssonL. A review of nanostructured Ca-aluminate based biomaterials within odontology and orthopedics. J Korean Ceram Soc2018; 55(2): 95–107.
40.
ThorntonFJBarbulA. Healing in the gastrointestinal tract. Surg Clin North America1997; 77(3): 549–573.
41.
ThompsonSKChangEYJobeBA. Clinical review: healing in gastrointestinal anastomoses, part I. Microsurgery2006; 26(3): 131–136.
WenzelRN. Resistance of solid surfaces to wetting by water. Trans Faraday Soc1936; 28(8): 988–994.
46.
FederleWBarnesWJPBaumgartnerW, et al.Wet but not slippery: boundary friction in tree frog adhesive toe pads. J R Soc Interface2006; 3(10): 689–697.
47.
ZhangL. Bioinspired surgical grasper based on the strong wet attachment of tree frog’s toe pads. J Mech Eng2018; 54(17): 14.
48.
HoradeM. Establishment of cell patterning method utilising nanopore structure with cell non-adhesive effect. J Micromech Microeng2019; 30(3): 035001.
49.
MoussaHILoganMWongK, et al.Nanoscale-textured tantalum surfaces for mammalian cell alignment. Micromachines, 2018; 9(9): 464.
50.
ZhengQMaoLShiY, et al.Biocompatibility of Ti-6Al-4V titanium alloy implants with laser microgrooved surfaces. Mater Technol. Epub ahead of print.2020. DOI: 10.1080/10667857.2020.1816011
51.
HuYChenXChenJ, et al.The influence of crescent texture parameters on the axial force when drilling bone. Med Eng Phys2021; 87(1): 87–94.
52.
YangCMeiXTianY, et al.Modification of wettability property of titanium by laser texturing. Int J Adv Manuf Technol2016; 87(5): 1663–1670.
53.
SanthoshKSHiremathSS. Effect of surface roughness and surface topography on wettability of machined biomaterials using flexible viscoelastic polymer abrasive media. Surf Topogr Metrol Prop2019; 7: 015004.
54.
MitratSKHansonDASchlaepferDD. Focal adhesion kinase: in command and control of cell motility. Nat Rev Mol Cell Biol2005; 6(1): 56–68.
55.
CerioneA. Cdc42: new roads to travel.. Trends Cell Biol2004; 14: 127–132.
56.
LaureR. Type I collagen purification from rat tail tendons. Methods Mol Biol2017; 1627: 287–308.
57.
SaeidGYeganehBZekiAA, et al.Autophagy and the unfolded protein response promote profibrotic effects of TGF-β in human lung fibroblasts.. Am J Physiol Lung Cell Mol Physiol2018; 314: L493–L504.
58.
KrauseACowlesE AGronowiczG. Integrin-mediated signaling in osteoblasts on titanium implant materials. J Biomed Mater Res2000; 52: 738–747.
