Dual functions of epigallocatechin gallate surface-modified Au nanorods@selenium composites for near-infrared-II light-responsive synergistic antibacterial therapy
Restricted accessResearch articleFirst published online May, 2022
Dual functions of epigallocatechin gallate surface-modified Au nanorods@selenium composites for near-infrared-II light-responsive synergistic antibacterial therapy
Diseases caused by bacterial infections pose ever-increasing threats to human health, making it important to explore alternative antibacterial strategies. Herein, epigallocatechin gallate (EGCG) surface-modified Au nanorods@selenium composites (ASE NPs) were developed for synergistic NIR-II light-responsive antibacterial therapy. In vitro antibacterial experiments demonstrated the improved antibacterial effect of ASE NPs against Staphylococcus aureus (S. aureus) compared with EGCG alone. In addition, in vivo studies demonstrated that ASE NPs cured skin wound infections and sepsis in mice caused by S. aureus. Au nanorods with excellent photothermal conversion realized synergistic photothermal therapy (PTT) in the NIR-II biowindow with an improved penetration depth at a low power density. More importantly, toxicity analysis showed that the composites had no toxic effects on major organs. Thus, the EGCG surface-modified Au nanorods@selenium composites with an NIR-II light-responsive synergistic activity hold great promise for the effective treatment of drug-resistant bacterial infections.
GaoNChenYJiangJ. Ag@Fe2O3-GO nanocomposites prepared by a phase transfer method with long-term antibacterial property. ACS Appl Mater Inter2013; 5: 11307–11314.
2.
LeiHWangCWangY, et al.Single-cell RNA-Seq revealed profound immune alteration in the peripheral blood of patients with bacterial infection. Int J Infect Dis2021; 103: 527–535.
3.
GeCWuRChongY, et al.Synthesis of pt hollow nanodendrites with enhanced peroxidase‐like activity against bacterial infections: implication for wound healing. Adv Funct Mater2018; 28: 18014841.
4.
LindsayJA. Hospital-associated MRSA and antibiotic resistance—what have we learned from genomics?Int J Med Microbiol2013; 303: 318–323.
5.
NielsHThomasBMichaelG, et al.Antibiotic resistance of bacterial biofilms. Int J Antimicrob Ag2010; 35: 322–332.
6.
LiDGaoXHuangX, et al.Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability. Carbohydr Polym2020; 249: 116858.
7.
CobleyCMChenJChoEC, et al.Gold nanostructures: a class of multifunctional materials for biomedical applications. Chem Soc Rev2011; 40: 44–56.
8.
ZhangZWangJNieX, et al.Near infared laser-induced targeted cancer therapy using thermoresponsive polymer encapsulated gold nanorods. J Am Chem Soc2014; 136: 7317–7326.
9.
ShuklaRBansalVChaudharyM, et al.Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir2005; 21: 10644–10654.
10.
LiXRobinsonSMGuptaA, et al.Functional gold nanoparticles as potent antimicrobial agents against multi-drug-resistant bacteria. ACS Nano2014; 8: 10682–10686.
11.
NormanRSStoneJWGoleA, et al.Targeted photothermal lysis of the pathogenic bacteria, Pseudomonas aeruginosa, with gold nanorods. Nano Lett2008; 8: 302–306.
12.
HuBWangNHanL, et al.Core-shell-shell nanorods for controlled release of silver that can serve as a nanoheater for photothermal treatment on bacteria. Acta Biomater2015; 11: 511–519.
CuiDMaJLiangT, et al.Selenium nanoparticles fabricated in laminarin polysaccharides solutions exert their cytotoxicities in HepG2 cells by inhibiting autophagy and promoting apoptosis. Int J Biol Macromolecules2019; 137: 829–835.
15.
AmeneSMaryamMAtefehDA, et al.Systematic review and meta-analysis shows a specific micronutrient profile in people with down syndrome: lower blood calcium, selenium and zinc, higher red blood cell copper and zinc, and higher salivary calcium and sodium. Plos One2017; 12: e0175437.
16.
VincetiMFilippiniTCilloniS, et al.Health risk assessment of environmental selenium: emerging evidence and challenges. Mol Med Rep2017; 15: 3323–3335.
17.
YazhiniprabhaMVaseeharanB. In vitro and in vivo toxicity assessment of selenium nanoparticles with significant larvicidal and bacteriostatic properties. Mater Sci Eng C, Mater Biol Appl2019; 103: 1097631–10976313.
18.
GaoXLiXMuJ, et al.Preparation, physicochemical characterization, and anti-proliferation of selenium nanoparticles stabilized by polyporus umbellatus polysaccharide. Int J Biol Macromolecules2020; 152: 605–615.
19.
JoshiSDe BrittoSJogaiahS, et al.Mycogenic selenium nanoparticles as potential new generation broad spectrum antifungal molecules. Biomolecules2019; 9: 419.
20.
AdadiPBarakovaNVMuravyovKY, et al.Designing selenium functional foods and beverages: a review. Food Res Int2019; 120: 708–725.
21.
HaoXBoseMLambertJD, et al.Inhibition of intestinal tumorigenesis inApcMin/+Mice by green tea polyphenols (polyphenon E) and individual catechins. Nutr Cancer2007; 59: 62–69.
22.
FriedmanMHenikaPRLevinCE, et al.Antimicrobial activities of tea catechins and theaflavins and tea extracts against bacillus cereus†. J Food Prot2006; 69: 354–361.
23.
CarneiroBMBatistaMNBragaACS, et al.The green tea molecule EGCG inhibits zika virus entry. Virology2016; 496: 215–218.
24.
LiuD-CZhangH-JJinC-M, et al.Synthesis and biological evaluation of novel benzothiazole derivatives as potential anticonvulsant agents. Molecules2016; 21: 164.
25.
ArakawaHMaedaMOkuboS, et al.Role of hydrogen peroxide in bactericidal action of catechin. Biol Pharm Bull2004; 27: 277–281.
26.
AkagawaMShigemitsuTSuyamaK. Production of hydrogen peroxide by polyphenols and polyphenol-rich beverages under quasi-physiological conditions. Biosci Biotechnol Biochem2003; 67: 2632–2640.
27.
ShiMNieYZhengX-Q, et al.Ultraviolet B (UVB) photosensitivities of tea catechins and the relevant chemical conversions. Molecules2016; 21: 1345.
28.
RenYWeiXWeiST, et al.High oxidation stability of tea polyphenol-stabilized highly crosslinked UHMWPE under an in vitro aggressive oxidative condition. Clin orthopaedics Relat Res2019; 477: 1947–1955.
29.
BhushaniJAKarthikPAnandharamakrishnanC. Nanoemulsion based delivery system for improved bioaccessibility and CaCo-2 cell monolayer permeability of green tea catechins. Food Hydrocolloids2016; 56: 372–382.
30.
ShimS-MYooS-HRaC-S, et al.Digestive stability and absorption of green tea polyphenols: Influence of acid and xylitol addition. Food Res Int2012; 45: 204–210.
31.
YangYJinPZhangX, et al.New epigallocatechin gallate (EGCG) nanocomplexes co-assembled with 3-Mercapto-1-Hexanol and β-Lactoglobulin for improvement of antitumor activity. J Biomed Nanotechnology2017; 13: 805–814.
32.
LiaoBYingHYuC, et al.(−)-Epigallocatechin gallate (EGCG)-nanoethosomes as a transdermal delivery system for docetaxel to treat implanted human melanoma cell tumors in mice. Int J Pharmaceutics2016; 512: 22–31.
33.
SiddiquiIAAdhamiVMBharaliDJ, et al.Introducing nanochemoprevention as a novel approach for cancer control: proof of principle with green tea polyphenol epigallocatechin-3-gallate. Cancer Res2009; 69: 1712–1716.
34.
ShpigelmanAIsraeliGLivneyYD. Thermally-induced protein-polyphenol co-assemblies: beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG. Food Hydrocolloids2010; 24: 735–743.
35.
PalSMitraKAzmiS, et al.Towards the synthesis of sugar amino acid containing antimicrobial noncytotoxic CAP conjugates with gold nanoparticles and a mechanistic study of cell disruption. Org Biomol Chem2011; 9: 4806–4810.
36.
GuoZGuCFanX, et al.Fabrication of anti-human cardiac troponin I immunogold nanorods for sensing acute myocardial damage. Nanoscale Res Lett2009; 4: 1428–1433.
37.
ZhuG-YLuB-YZhangT-X, et al.Antibiofilm effect of drug-free and cationic poly(D,L-lactide-co-glycolide) nanoparticles via nano-bacteria interactions. Nanomedicine2018; 13: 1093–1106.
38.
PerihanA. Khalaf‐Alla. antioxidant, antimicrobial and antitumor studies of transition metal complexes derived from N‐(2‐Aminoethyl)‐1,3‐propanediamine with DFT calculations and molecular docking investigation. Appl Organometal Chem2020; 34: e5268.
39.
ChangwookMHuilinSMontyL, et al.Mechanism of magnetic relaxation switching sensing. ACS Nano2012; 6: 6821–6828.
40.
MoonS-EKimH-YChaJ-D. Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Arch Oral Biol2011; 56: 907–916.
41.
ZhaoYChenZChenY, et al.Synergy of non-antibiotic drugs and pyrimidinethiol on gold nanoparticles against superbugs. J Am Chem Soc2013; 135: 12940–12943.
42.
JiaTQianCLigengX, et al.Graphene oxide-silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms. ACS Appl Mater Inter2013; 5: 3867–3874.
43.
ZhaoYYeCLiuW, et al.Tuning the composition of AuPt bimetallic nanoparticles for antibacterial application. Angew Chem2014; 126: 8265–8269.
44.
SunDZhangWLiN, et al.Silver nanoparticles-quercetin conjugation to siRNA against drug-resistant bacillus subtilis for effective gene silencing: in vitro and in vivo. Mater Sci Eng C2016; 63: 522–534.
45.
CuiYLiuHZhouM, et al.Signaling pathway of inflammatory responses in the mouse liver caused by TiO2 nanoparticles. J Biomed Mater Res A2011; 96A: 221–229.
46.
ChoYSSchillerNLKahngHY, et al.Cellular responses and proteomic analysis of escherichia coli exposed to green tea polyphenols. Curr Microbiol2007; 55: 501–506.
47.
ZhangXZhangZShuQ, et al.Copper clusters: an Effective antibacterial for eradicating multidrug‐resistant bacterial infection in vitro and in vivo. Adv Funct Mater2021; 31: 2008720.
48.
SchmidtRYonghongDHoffmannR. Phospholipid composition of the outer membrane of escherichia coli influences its susceptibility against antimicrobial peptide apidaecin 1b. Diagn Microbiol Infect Dis2018; 90: 316–323.
49.
AlkilanyAMThompsonLBBoulosSP, et al.Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions. Adv Drug Deliv Rev2012; 64: 190–199.
50.
ZhouYBaoQTangLAL, et al.Hydrothermal dehydration for the “green” reduction of exfoliated graphene oxide to graphene and demonstration of tunable optical limiting properties. Chem Mater2009; 21: 2950–2956.
