Three types of Co-xZr (x = 5, 7.5, and 10 wt.%) were treated with hydroxyapatite (HA) and used as an object to investigate the effect of HA coating on the surface and biocompatibility of Co-xZr alloys. And the protein adsorption and the subsequent biological behaviour of osteoblast, fibroblast and macrophages were also investigated. The surface microstructure and wettability were assessed by scanning electron microscopy (SEM) and static angle profilometer. To evaluate the biocompatibility of Co-xZr and Co-xZr-HA, we quantified plasma proteins adsorption by bicinchoninic acid assay (BCA), cytotoxicity and cell proliferation by cell counting kit-8 (CCK-8) and scanning electron microscopy (SEM). The results indicated that Co-xZr-HA alloy surfaces were more hydrophilic and had higher affinity to plasma proteins. Higher protein concentrations were found adsorbed onto Co-7.5Zr-HA and Co-10Zr-HA alloys. Cytotoxicity analysis indicated that HA coating improved the biocompatibility of Co-xZr alloys. Furthermore, the comparable results of co-incubation of Co-xZr-HA alloys with cells reveal cellular attachments to HA surfaces. HA was successfully formed on Co-xZr alloys and modified the surface structure and biocompatibility of the alloys. Co-10Zr-HA and Co-7.5Zr-HA had the most favourable properties and cytocompatibility, and therefore can be potentially used for dental implants.
LiuXYChuPKDingCX.Surface modification of titanium, titanium alloys, and related materials for biomedical applications. Mat Sci Eng R2004;
47: 49–121.
2.
LongMRackHJ.Titanium alloys in total joint replacement–a materials science perspective. Biomaterials1998;
19: 1621–1639.
3.
PalmquistAOmarOMEspositoM, et al.
Titanium oral implants: surface characteristics, interface biology and clinical outcome. J R Soc Interface2010;
7: S515–S527.
4.
NiinomiMNakaiMHiedaJ.Development of new metallic alloys for biomedical applications. Acta Biomater2012;
8: 3888–3903.
5.
ChangLLiuJYangHL, et al.
Effects of Zr contents on the microstructure, mechanical properties and biocompatibility of Ta-Zr alloys. Mater Sci Forum2018;
914: 37–44.
6.
NiinomiMLiuYNakaiM, et al.
Biomedical titanium alloys with young's moduli close to that of cortical bone. Regen Biomater2016;
3: 173–185.
7.
RuanJMYangHLWengXJ, et al.
Preparation and characterization of biomedical highly porous Ti-Nb alloy. J Mater Sci2016;
27: 76–83.
8.
RabeMVerdesDSeegerS.Understanding protein adsorption phenomena at solid surfaces. Adv Colloid Interface Sci2011;
162: 87–106.
9.
CaiKYFrantMBossertJ, et al.
Surface functionalized titanium thin films: Zeta-potential, protein adsorption and cell proliferation. Colloids Surf B Biointerfaces2006;
50: 1–8.
10.
ISO 10993-5:2009. Biological evaluation of medical devices – part 5: tests for in vitro cytotoxicity.
LiuXDingCChuPK.Mechanism of apatite formation on wollastonite coatings in simulated bodyfluids. Biomaterials2004;
25: 1755–1761.
13.
YamashitaKOikawaNUmegakiT.Acceleration and deceleration of bone-like crystal growth on ceramic hydroxyapatite by electric poling. Chem Mater1996;
8: 2697–2700.
14.
KaranjaiMSundaresanRMohanTRR, et al.
Evaluation of growth of Ca–P ceramics on sintered Ti–Ca–P composites. Mater Sci Eng C2008;
28: 1401–1407.
15.
PaitalSRDahotreNB.Wettability and kinetics of hydroxyapatite precipitation on a laser-textured Caecibioceramic coating. Acta Biomater2009;
5: 2763–2772.
16.
HuynhVNgoNKGoldenTD.Surface activation and pretreatments for biocompatible metals and alloys used in biomedical applications. Int J Biomater2019;
2019: 3806504.
17.
LinF-HHsuY-SLinS-H, et al.
The effect of Ca/P concentration and temperature of simulated body fluid on the growth of hydroxyapatite coating on alkali-treated 316L stainless steel. Biomaterials2002;
23: 4029–4038.
18.
Marashi-NajafiFKhalil-AllafiJEtminanfarMR.Biocompatibility of hydroxyapatite coatings deposited by pulse electrodeposition technique on the nitinol superelastic alloy. Mater Sci Eng C Mater Biol Appl2017;
76: 278–286.
19.
SmeetsRStadlingerBSchwarzF, et al.
Impact of dental implant surface modifications on osseointegration. Biomed Res Int2016;
2016: 6285620.10.1155/2016/6285620.
20.
TerheydenHLangNPBierbaumS, et al.
Osseointegration – communication of cells. Clin Oral Implants Res2012;
23: 1127–1135.
21.
SinghBSinghGSidhuBS.Analysis of corrosion behavior and surface properties of plasma-sprayed HA/ta coating on CoCr alloy. J Therm Spray Tech2018;
27: 1401–1413.
22.
DurduSKorkmazKAktuğSL, et al.
Characterization and bioactivity of Hydroxyapatite-Based coatings formed on steel by electro-spark deposition and micro-arc oxidation. Surf Coat Technol2017;
326: 111–120.
23.
HamtaipourSIzmanSHessamH.Evaluating the effect of pretreatment parameters on Co-Cr-Mo alloy prior to physical vapor deposition coating. J Mekanikal2013;
36: 34–41.
24.
MiyazakiTKimHMKokuboT, et al.
Mechanism of bonelike apatite formation on bioactive tantalum metal in a simulated body fluid. Biomaterials2002;
23: 827–832.
25.
TakemotoMFujibayashiSNeoM, et al.
A porous bioactive titanium implant for spinal interbody fusion: an experimental study using a canine model. J Neurosurg Spine2007;
7: 435–443.
26.
BarradasAMCFernandesHAMGroenN, et al.
A calcium-induced signaling Cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells. Biomaterials2012;
33: 3205–3215.
27.
ZhuXDFanHSXiaoYM, et al.
Effect of surface structure on protein adsorption to biphasic calcium-phosphate ceramics in vitro and in vivo. Acta Biomater2009;
5: 1311–1318.
28.
WangKZhouCHongY, et al.
A review of protein adsorption on bioceramics. Interface Focus2012;
2: 259–277.
29.
RoachPEglinDRohdeK, et al.
Modern biomaterials: a review-bulk properties and implications of surface modifications. J Mater Sci Mater Med2007;
18: 1263–1277.
30.
FengBChenJZhangX.Interaction of calcium and phosphate in apatite coating on titanium with serum albumin. Biomaterials2002;
23: 2499–2507.
31.
LiYLayrollePDBruijnJ, et al.
Biomimetic coprecipitation of calcium phosphate and bovine serum albumin on titanium alloy. J Mater Res2001;
57: 327–335.
32.
YangYZCavinROngJL.Protein adsorption on titanium surfaces and their effect on osteoblast attachment. J Biomed Mater Res A2003;
67: 344–349.
33.
DalalAPawarVMcAllisterK, et al.
Orthopedic implant cobalt-alloy particles produce greater toxicity and inflammatory cytokines than titanium alloy and zirconium alloy-based particles in vitro, in human osteoblasts, fibroblasts, and macrophages. J Biomed Mater Res A2012;
100a: 2147–2158.
34.
KwonYMXiaZGlyn-JonesS, et al.
Dose-dependent cytotoxicity of clinically relevant cobalt nanoparticles and ions on macrophages in vitro. Biomed Mater2009;
4: 025018.
35.
RajivSJerobinJSaranyaV, et al.
Comparative cytotoxicity and genotoxicity of cobalt (II, III) oxide, iron (III) oxide, silicon dioxide, and aluminum oxide nanoparticles on human lymphocytes in vitro. Hum Exp Toxicol2016;
35: 170–183.
