Restricted accessResearch articleFirst published online 2020-10
Effect of different exposure times on physicochemical,mechanical and biological properties of PGS scaffolds treated with plasma of iodine-doped polypyrrole
Polyglycerol sebacate (PGS) scaffolds obtained using a leaching technique were modified with iodine-doped polypyrrole (PPy-I) in a plasma reactor in order to study the effect of exposure time on the cell viability of hDPSCs. SEM analysis showed the formation and growth of PPy-I particles as the exposure time was increased, while FTIR and XPS analysis revealed the presence of -NH- and N+ groups in the chemical composition of the surfaces, relating to the increase in the amount of PPY-I particles. The water contact angle measurements showed an increase in the scaffold’s hydrophilicity with greater exposure times which was also attributed to the rising of PPy-I particles. It was also observed that PPy-I promotes the rigidity of the treated PGS scaffolds. when in direct contact with treated PGS scaffolds, cell viability improved with respect to non-treated scaffolds, however only at shorter time exposures. Extracts of plasma-treated PGS scaffolds showed high cytotoxicity as the time exposure to plasma treatment was increased.
ChungHJParkTG.Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering. Adv Drug Deliv Rev2007;
59: 249–262.
2.
WangYAmeerGASheppardBJ, et al.
A tough biodegradable elastomer. Nat Biotechnol2002;
20: 602–606.
3.
RaiRTallawiMGrigoreA, et al.
Synthesis, properties and biomedical applications of poly (glycerol sebacate)(PGS): a review. Progr Polym Sci2012;
37: 1051–1078.
4.
KemppainenJMHollisterSJ.Tailoring the mechanical properties of 3D‐designed poly (glycerol sebacate) scaffolds for cartilage applications. J Biomed Mater Res A2010;
94: 9–18.
5.
Theerathanagorn T, Thavornyutikarn B and Janvikul W. Preparation and Characterization of Plasma-treated Porous Poly (glycerol sebacate) Scaffolds. Advanced Materials Research. T 2013; 747: 182–185.
6.
ChenQ-ZBismarckAHansenU, et al.
Characterisation of a soft elastomer poly (glycerol sebacate) designed to match the mechanical properties of myocardial tissue. Biomaterials2008;
29: 47–57.
7.
ChenQ-ZIshiiHThouasGA, et al.
An elastomeric patch derived from poly (glycerol sebacate) for delivery of embryonic stem cells to the heart. Biomaterials2010;
31: 3885–3893.
8.
SantSIyerDGaharwarAK, et al.
Effect of biodegradation and de novo matrix synthesis on the mechanical properties of valvular interstitial cell-seeded polyglycerol sebacate–polycaprolactone scaffolds. Acta Biomater2013;
9: 5963–5973.
9.
MotlaghDYangJLuiKY, et al.
Hemocompatibility evaluation of poly (glycerol-sebacate) in vitro for vascular tissue engineering. Biomaterials2006;
27: 4315–4324.
10.
SundbackCAShyuJYWangY, et al.
Biocompatibility analysis of poly (glycerol sebacate) as a nerve guide material. Biomaterials2005;
26: 5454–5464.
11.
PritchardCDArnérKMLangerRS, et al.
Retinal transplantation using surface modified poly (glycerol-co-sebacic acid) membranes. Biomaterials2010;
31: 7978–7984.
12.
LiYCookWDMoorhoffC, et al.
Synthesis, characterization and properties of biocompatible poly (glycerol sebacate) pre‐polymer and gel. Polym Int2013;
62: 534–547.
13.
OlayoRRíosCSalgado-CeballosH, et al.
Tissue spinal cord response in rats after implants of polypyrrole and polyethylene glycol obtained by plasma. J Mater Sci Mater Med2008;
19: 817–826.
14.
Ramírez-Fernández, O. et al. Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds. Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electrónica 2015; 39: 9–14.
15.
Zuñiga-AguilarEOlayoRRamírez-FernándezO, et al.
Nerve cells culture from lumbar spinal cord on surfaces modified by plasma pyrrole polymerization. J Biomater Sci Polym Ed2014;
25: 729–747.
16.
SerratosINOlayoRMillán-PachecoC, et al.
Modeling integrin and plasma-polymerized pyrrole interactions: chemical diversity relevance for cell regeneration. Sci Rep2019;
9: 1–12.
17.
Islas-ArteagaNCRaya RiveraAEsquiliano RendonDR, et al.
Electrospun scaffolds with surfaces modified by plasma for regeneration of articular cartilage tissue: a pilot study in rabbit. Int J Polym Mater Polym Biomater2019;
68: 1089–1098.
18.
Alvarez-MejiaLMoralesJCruzGJ, et al.
Functional recovery in spinal cord injured rats using polypyrrole/iodine implants and treadmill training. J Mater Sci Mater Med2015;
26: 209.
19.
RungeMBDadsetanMBaltrusaitisJ, et al.
The development of electrically conductive polycaprolactone fumarate–polypyrrole composite materials for nerve regeneration. Biomaterials2010;
31: 5916–5926.
20.
Román-DovalRTellez-CruzMMRojas-ChávezH, et al.
Enhancing electrospun scaffolds of PVP with polypyrrole/iodine for tissue engineering of skin regeneration by coating via a plasma process. J Mater Sci2019;
54: 3342–3353.
21.
Flores-SánchezMGRaya-RiveraAMEsquiliano-RendonDR, et al.
Scaffolds of polylactic acid/hydroxyapatite coated by plasma with polypyrrole-iodine for the generation of neo-tissue–bone in vivo: study in rabbit. Int J Polym Mater Polym Biomater2018;
67: 427–437.
22.
CruzYMuñozEGomez-PachónEY, et al.
Electrospun PCL-protein scaffolds coated by pyrrole plasma polymerization. J Biomater Sci Polym Ed2019;
30: 832–845.
23.
ZhangXJiaCQiaoX, et al.
Porous poly (glycerol sebacate) (PGS) elastomer scaffolds for skin tissue engineering. Polymer Test2016;
54: 118–125.
24.
International Organization for Standardization ISO 10993-12. Biological evaluation of medical devices-Part 12: Sample preparation and reference materials 2012.
25.
CruzGJOlayoMGLópezOG, et al.
Nanospherical particles of polypyrrole synthesized and doped by plasma. Polymer2010;
51: 4314–4318.
26.
González-TorresMOlayoMGCruzGJ, et al.
XPS study of the chemical structure of plasma biocopolymers of pyrrole and ethylene glycol. Adv Chem2014;
2014: 1–8.
27.
PaosawatyanyongBTapaneeyakornKBhanthumnavinW.AC plasma polymerization of pyrrole. Surface Coat Technol2010;
204: 3069–3072.
28.
WangYSotzingGAWeissR.Preparation of conductive polypyrrole/polyurethane composite foams by in situ polymerization of pyrrole. Chem Mater2008;
20: 2574–2582.
29.
WangJNeohKKangE.Comparative study of chemically synthesized and plasma polymerized pyrrole and thiophene thin films. Thin Solid Films2004;
446: 205–217.
30.
BazakaKJacobM.Effects of iodine doping on optoelectronic and chemical properties of polyterpenol thin films. Nanomaterials2017;
7: 11.
31.
ShiHGanQLiuX, et al.
Poly (glycerol sebacate)-modified polylactic acid scaffolds with improved hydrophilicity, mechanical strength and bioactivity for bone tissue regeneration. RSC Adv2015;
5: 79703–79714.
32.
WalterC, et al.
Structural investigations of composites produced from copper and polypyrrole with a dual PVD/PE‐CVD process. Plasma Process Polym2009;
6: NA–812.
33.
SuNLiHBYuanSJ, et al.
Synthesis and characterization of polypyrrole doped with anionic spherical polyelectrolyte brushes. Express Polym Lett2012;
6: 697–705.
34.
KravetsLIGilmanABYablokovMY, et al.
A novel technique for fabrication of nanofluidic devices with polymer film formed by plasma polymerization. High Temp Mat Proc2015;
19: 1–18.
35.
YagüeJLBorrósS.Conducting plasma polymerized polypyrrole thin films as carbon dioxide gas sensors. Plasma Processes Polym2012;
9: 485–490.
36.
ParkC-SJungEKimD, et al.
Atmospheric pressure plasma polymerization synthesis and characterization of polyaniline films doped with and without iodine. Materials2017;
10: 1272.
GaoJCrapoPMWangY.Macroporous elastomeric scaffolds with extensive micropores for soft tissue engineering. Tissue Eng2006;
12: 917–925.
40.
GuYBaiYZhangD.Osteogenic stimulation of human dental pulp stem cells with a novel gelatin‐hydroxyapatite‐tricalcium phosphate scaffold. J Biomed Mater Res A2018;
106: 1851–1861.
41.
MiyashitaSAhmedNEMBMurakamiM, et al.
Mechanical forces induce odontoblastic differentiation of mesenchymal stem cells on three‐dimensional biomimetic scaffolds. J Tissue Eng Regen Med2017;
11: 434–446.
42.
HanJKimDSJangH, et al.
Bioprinting of three-dimensional dentin–pulp complex with local differentiation of human dental pulp stem cells. J Tissue Eng2019;
10: 2041731419845849.
43.
KwonDYKwonJSParkSH, et al.
A computer-designed scaffold for bone regeneration within cranial defect using human dental pulp stem cells. Sci Rep2015;
5: 12721.
44.
JiménezNTCarlos MunévarJGonzálezJM, et al.
In vitro response of dental pulp stem cells in 3D scaffolds: a regenerative bone material. Heliyon2018;
4: e00775.
45.
ChengY-C, et al.
Electrical stimulation through conductive substrate to enhance osteo-differentiation of human dental pulp-derived stem cells. Appl Sci2019;
9: 3938.
Fabela-SánchezOSalgado-CeballosHMedina-TorresL, et al.
Effect of the combined treatment of albumin with plasma synthesised pyrrole polymers on motor recovery after traumatic spinal cord injury in rats. J Mater Sci Mater Med2017;
29: 13.
48.
HongYLiYZhuangX, et al.
Electrospinning of multicomponent ultrathin fibrous nonwovens for semi‐occlusive wound dressings. J Biomed Mater Res A2009;
89: 345–354.
49.
WallinRFArscottE.A practical guide to ISO 10993-5: cytotoxicity. Med Dev Diagn Ind1998;
20: 96–98.
50.
LeeJYSchmidtCE.Amine‐functionalized polypyrrole: inherently cell adhesive conducting polymer. J Biomed Mater Res A2015;
103: 2126–2132.
51.
ColínEOlayoMGCruzGJ, et al.
Affinity of amine-functionalized plasma polymers with ionic solutions similar to those in the human body. Progr Organ Coat2009;
64: 322–326.
52.
Sánchez-PechJCRosales-IbáñesRCauich-RodriguezJV, et al.
Design, synthesis, characterization, and cytotoxicity of PCL/PLGA scaffolds through plasma treatment in the presence of pyrrole for possible use in urethral tissue engineering. J Biomater Appl2020;
34: 840–850.
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