Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction

Abstract

Introduction and hypothesis: Pelvic support structure injury is the major cause of pelvic organ prolapse. At present, polypropylene-based filler material has been suggested as a common method to treat pelvic organ prolapse. However, it cannot functionally rehabilitate the pelvic support structure. In addition to its poor long-term efficiency, the urinary bladder matrix was the most suitable biological scaffold material for pelvic floor repair. Here, we hypothesize that anti-sca-1 monoclonal antibody and basic fibroblast growth factor were cross-linked to urinary bladder matrix to construct a two-factor bioscaffold for pelvic reconstruction.

Methods

Through a bispecific cross-linking reagent, sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-smcc) immobilized anti-sca-1 and basic fibroblast growth factor to urinary bladder matrix. Then scanning electron microscope and plate reader were used to detect whether the anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold was built successfully. After that, the capacity of enriching sca-1 positive cells was measured both in vitro and in vivo. In addition, we evaluated the differentiation capacity and biocompatibility of the scaffold. Finally, western blotting was used to detect the level of fibulin-5 protein.

Results

The scanning electron microscope and plate reader revealed that the double-factor biological scaffold was built successfully. The scaffold could significantly enrich a large number of sca-1 positive cells both in vitro and in vivo, and obviously accelerate cells and differentiate functional tissue with good biocompatibility. Moreover, the western blotting showed that the scaffold could improve the expression of fibulin-5 protein.

Conclusion

The anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold revealed good biological properties and might serve as an ideal scaffold for pelvic reconstruction.

Keywords

Pelvic organ prolapse urinary bladder matrix basic fibroblast growth factor anti-sca-1 sulfo-smcc

Get full access to this article

View all access options for this article.

References

Chaliha

Khullar

Surgical repair of vaginal prolapse: a gynaecological hernia. Int J Surg 2006; 4: 242–250.

Khadzhieva

et al . Fibulin-5 (FBLN5) gene polymorphism is associated with pelvic organ prolapse. Maturitas 2014; 78: 287–292.

Wong

et al . Adverse events associated with pelvic organ prolapse surgeries that use implants. Obstet Gynecol 2013; 122: 1239–1245.

Yesil

Watermann

Farthmann

Mesh implantation for pelvic organ prolapse improves quality of life. Arch Gynecol Obstet 2014; 289: 817–821.

Liu

et al . Evaluation of the biocompatibility and mechanical properties of xenogeneic (porcine) extracellular matrix (ECM) scaffold for pelvic reconstruction. Int Urogynecol J 2011; 22: 221–227.

Crapo

Gilbert

Badylak

SF.

An overview of tissue and whole organ decellularization processes. Biomaterials 2011; 32: 3233–3243.

Badylak

et al . Engineered whole organs and complex tissues. Lancet 2012; 379: 943–952.

Bible

et al . Non-invasive imaging of transplanted human neural stem cells and ECM scaffold remodeling in the stroke-damaged rat brain by 19F- and diffusion-MRI. Biomaterials 2012; 33: 2858–2871.

Aurora

et al . An acellular biologic scaffold does not regenerate appreciable de novo muscle tissue in rat models of volumetric muscle loss injury. Biomaterials 2015; 67: 393–407.

10.

Boruch

et al . Constructive remodeling of biologic scaffolds is dependent on early exposure to physiologic bladder filling in a canine partial cystectomy model. J Surgical Res 2010; 161: 217–225.

11.

Kim

et al . A healing method of tympanic membrane perforations using three-dimensional porous chitosan scaffolds. Tissue Eng 2011; 17: 2763–2772.

12.

Yurteri-Kaplan

Gutman

RE.

The use of biological materials in urogynecologic reconstruction. Plastic Reconstruct Surg 2012; 130: 242S–253S.

13.

Fan

et al . Comparison of polypropylene mesh and porcine-derived, cross-linked urinary bladder matrix materials implanted in the rabbit vagina and abdomen. Int Urogynecol J 2014; 25: 683–689.

14.

Shi

et al . Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration. Biomaterials 2011; 32: 2508–2515.

15.

Xue

et al . Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing. Oncotarget 2017; 26: 42098–42115.

16.

Takeshita

et al . Xenotransplantation of interferon-gamma pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther 2017; 8: 101.

17.

Chamieh

et al . Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells. Scientific Report 2016; 6: 38814.

18.

Wang

et al . A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo. Int J Mol Med 2017; 39: 167–173.

19.

Chen

et al . Bladder acellular matrix conjugated with basic fibroblast growth factor for bladder regeneration. Tissue Eng 2014; 20: 2234–2242.

20.

Chen

et al . Bladder regeneration by collagen scaffolds with collagen binding human basic fibroblast growth factor. J Urol 2010; 183: 2432–2439.

21.

Pokrywczynska

et al . Application of bladder acellular matrix in urinary bladder regeneration: the state of the art and future directions. BioMed Res Int 2015; 2015: 1–11.

22.

Shi

et al . Regeneration of full-thickness abdominal wall defects in rats using collagen scaffolds loaded with collagen-binding basic fibroblast growth factor. Biomaterials 2011; 32: 753–759.

23.

Shi

et al . Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model. Sci China Life Sci 2014; 57: 232–240.

24.

et al . Control-released basic fibroblast growth factor-loaded poly-lactic-co-glycolic acid microspheres promote sciatic nerve regeneration in rats. Exp Therapeut Med 2017; 13: 429–436.

25.

Momose

et al . Collagen hydrogel scaffold and fibroblast growth factor-2 accelerate periodontal healing of Class II furcation defects in dog. Open Dent J 2016; 10: 347–359.

26.

Leong

et al . Evaluation of polycaprolactone scaffold with basic fibroblast growth factor and fibroblasts in an athymic rat model for anterior cruciate ligament reconstruction. Tissue Eng Part A 2015; 21: 11–12.

27.

AE, Rundell

et al . Biaxial strength of multilaminated extracellular matrix scaffolds. Biomaterials 2004; 25: 2353–2361.

28.

Zhu

et al . A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Nat Protoc. 2010; 5: 550–560.

29.

Bayrak

et al . Human immune responses to porcine xenogeneic matrices and their extracellular matrix constituents in vitro. Biomaterials 2010; 31: 3793–3803.

30.

Bolland

et al . Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomaterials 2007; 28: 1061–1070.

31.

Drewes

et al . Pelvic organ prolapse in fibulin-5 knockout mice. Am J Pathol 2007; 170: 578–589.

32.

Budatha

et al . Extracellular matrix proteases contribute to progression of pelvic organ prolapse in mice and humans. J Clin Invest 2011; 121: 2048–2059.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.09 MB