Recently different hemostatic agents have been developed, but most of them are ineffective in severe bleeding and expensive or cause safety concerns. In this study, we fabricated duck's feet collagen-based porous sponges and investigated its use as a hemostatic agent. We determined the sponge's physical and biological characteristics and compared with Avitene via scanning electron microscope analysis, water-uptake abilities and porosity test, and cytotoxicity assay. The duck's feet collagen/silk sponge showed a larger interconnected porous structure compared to others sponges. The duck's feet collagen/silk sponge also exhibited significantly higher porosity than Avitene. Hemostatic properties of the sponges were evaluated by whole blood clotting and rat femoral artery hemorrhage experiment. The addition of silk to duck's feet collagen showed better blood clotting ability than Avitene in vitro. However, rat femoral artery hemorrhage test showed a similar hemostatic property between the duck's feet collagen-based sponges and Avitene. We suggest that duck's feet collagen-based sponge can be effectively used for hemostatic applications.
SundaramCPKeenanAC. Evolution of hemostatic agents in surgical practice. Indian J Urol2010; 26: 374–378.
2.
LandryJRKanatIO. Considerations in topical hemostasis. J Am Podiatr Med Assoc1985; 75: 581–585.
3.
BoatengJSMatthewsKHStevensHNet al.Wound healing dressings and drug delivery systems: a review. J Pharm Sci2008; 97: 2892–2923.
4.
IgaiHYamamotoYChangSSet al.Tracheal cartilage regeneration by slow release of basic fibroblast growth factor from a gelatin sponge. J Thorac Cardiovasc Surg2007; 134: 170–175.
5.
BjorsesKHolstJ. Various local hemostatic agents with different modes of action; an in vivo comparative randomized vascular surgical experimental study. Eur J Vasc Endovasc Surg2007; 33: 363–370.
6.
SchonauerCTessitoreEBarbagalloGet al.The use of local agents: bone wax, gelatin, collagen, oxidized cellulose. Eur Spine J2004; 13: S89–S96.
7.
ValeriCRVournakisJN. mRDH bandage for surgery and trauma: data summary and comparative review. J Trauma2011; 71: S162–S166.
8.
Granville-ChapmanJJacobsNMidwinterMJ. Pre-hospital haemostatic dressings: a systematic review. Injury2011; 42: 447–459.
9.
ClayJGGraysonJKZieroldD. Comparative testing of new hemostatic agents in a swine model of extremity arterial and venous hemorrhage. Mil Med2010; 175: 280–284.
10.
DevlinJJKircherSKozenBGet al.Comparison of ChitoFlex(R), CELOX, and QuikClot(R) in control of hemorrhage. J Emerg Med2011; 41: 237–245.
11.
WaibelKHHaneyBMooreMet al.Safety of chitosan bandages in shellfish allergic patients. Mil Med2011; 176: 1153–1156.
12.
BilgiliHKosarAKurtMet al.Hemostatic efficacy of Ankaferd blood stopper in a swine bleeding model. Med Princ Pract2009; 18: 165–169.
13.
ArnaudFTomoriTCarrWet al.Exothermic reaction in zeolite hemostatic dressings: QuikClot ACS and ACS+. Ann Biomed Eng2008; 36: 1708–1713.
14.
RheePBrownCMartinMet al.QuikClot use in trauma for hemorrhage control: case series of 103 documented uses. J Trauma2008; 64: 1093–1099.
O'ShaughnessyBASchafernakKTDiPatriAJJret al.A granulomatous reaction to avitene mimicking recurrence of a medulloblastoma. Case report. J Neurosurg2006; 104: 33–36.
17.
NakajimaMKameiTTomimatuKet al.An intraperitoneal tumorous mass caused by granulomas of microfibrillar collagen hemostat (Avitene). Arch Pathol Lab Med1995; 119: 1161–1163.
18.
KitamuraKYasuokaROharaMet al.How safe are the xenogeneic hemostats? – report of a case of severe systemic allergic reaction. Surg Today1995; 25: 433–435.
19.
OgawaMPortierRJMoodyMWet al.Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus). Food Chem2004; 88: 495–501.
20.
SultanMTJeongJYSeoYBet al.Fabrication and characterization of the porous duck's feet collagen sponge for wound healing applications. J Biomater Sci Polym Ed2017; 28: 1–12. .
21.
KimSHParkHSLeeOJet al.Fabrication of duck's feet collagen-silk hybrid biomaterial for tissue engineering. Int J Biol Macromol2016; 85: 442–450.
22.
LongKLiuYLiWet al.Improving the mechanical properties of collagen-based membranes using silk fibroin for corneal tissue engineering. J Biomed Mater Res A2015; 103: 1159–1168.
23.
LeeOJLeeJMKimJHet al.Biodegradation behavior of silk fibroin membranes in repairing tympanic membrane perforations. J Biomed Mater Res A2012; 100: 2018–2026.
24.
BiFShiZLiuAet al.Anterior cruciate ligament reconstruction in a rabbit model using silk-collagen scaffold and comparison with autograft. PLoS One2015; 10(5): e0125900.
25.
SunKLiHLiRet al.Silk fibroin/collagen and silk fibroin/chitosan blended three-dimensional scaffolds for tissue engineering. Eur J Orthop Surg Traumatol2015; 25: 243–249.
26.
MoonBMJuHWParkHJet al.The pilot study for the development of the hemostatic agent using silk fibroin for epistaxis. Tissue Eng Regenerative Med2013; 10: 1–7.
27.
LvQCaoCZhangYet al.The preparation of insoluble fibroin films induced by degummed fibroin or fibroin microspheres. J Mater Sci Mater Med2004; 15: 1193–1197.
28.
ZhangRMaPX. Poly(alpha-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology. J Biomed Mater Res1999; 44: 446–455.
ChengXShaoZLiCet al.Isolation, characterization and evaluation of collagen from jellyfish Rhopilema esculentum Kishinouye for use in hemostatic applications. PLoS One2017; 12: e0169731.
31.
MuyongaJHColeCGBDuoduKG. Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem2004; 86: 325–332.
32.
NeufferMCMcDivittJRoseDet al.Hemostatic dressings for the first responder: a review. Mil Med2004; 169: 716–720.
33.
AchneckHESileshiBJamiolkowskiRMet al.A comprehensive review of topical hemostatic agents: efficacy and recommendations for use. Ann Surg2010; 251: 217–228.
34.
SpotnitzWDBurksS. Hemostats, sealants, and adhesives: components of the surgical toolbox. Transfusion2008; 48: 1502–1516.
35.
SixmaJJvan ZantenGHHuizingaEGet al.Platelet adhesion to collagen: an update. Thromb Haemost1997; 78: 434–438.
36.
SirlakMEryilmazSYaziciogluLet al.Comparative study of microfibrillar collagen hemostat (Colgel) and oxidized cellulose (Surgicel) in high transfusion-risk cardiac surgery. J Thorac Cardiovasc Surg2003; 126: 666–670.
37.
LeiCZhuHLiJet al.Preparation and hemostatic property of low molecular weight silk fibroin. J Biomater Sci Polym Ed2016; 27: 403–418.
