This study sought to prepare biphasic mixtures by adding tricalcium aluminate (Ca3Al2O6) into dicalcium silicate (Ca2SiO4) and to evaluate the effect of Ca3Al2O6 on setting properties and biocompatibility of the Ca2SiO4/Ca3Al2O6 mixtures as compared to pure Ca2SiO4. The results indicated that the addition of Ca3Al2O6 into Ca2SiO4 reduced the setting time and improved the compressive strength. Furthermore, Ca2SiO4/Ca3Al2O6 mixtures were bioactive and biocompatible, and had a stimulatory effect on the L929 cell growth when the content of Ca3Al2O6 was below 10%. Therefore, the mixtures with 10% Ca3Al2O6 produced the best compromise between setting and in vitro biological properties.
RobertsHWTothJMBerzinsDWCharltonDG. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dent Mater2008; 24(2): 149–164.
2.
MitchellPJCFordTRPTorabinejadMMcDonaldF. Osteoblast biocompatibility of mineral trioxide aggregate. Biomaterials1999; 20(2): 167–173.
3.
KohETTorabinejadMFordTRPBradyKMcDonaldF. Mineral trioxide aggregate stimulates a biological response in human osteoblasts. J Biomed Mater Res1997; 37(3): 432–439.
4.
KohETMcDonaldFFordTRPTorabinejadM. Cellular response to mineral trioxide aggregate. J Endod1998; 24(8): 543–547.
5.
CintraLTAde MoraesIGEstradaBPFGomes-FilhoJEBramanteCMGarciaRB. Evaluation of the tissue response to MTA and MBPC: microscopic analysis of implants in alveolar bone of rats. J Endod2006; 32(6): 556–559.
6.
TorabinejadMHongC-ULeeS-JMonsefMPitt FordTR. Investigation of mineral trioxide aggregate for root-end filling in dogs. J Endod1995; 21(12): 603–608.
7.
TorabinejadMPitt FordTRAbediHRKariyawasamSPTangH-M. Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs. J Endod1998; 24(7): 468–471.
8.
SchwartzRSMaugerMClementDJWalkerWA. Mineral trioxide aggregate: a new material for endodontics. J Am Dent Assoc1999; 130(7): 967–975.
9.
ParirokhMTorabinejadM. Mineral trioxide aggregate: a comprehensive literature review-Part III: clinical applications, drawbacks, and mechanism of action. J Endod2010; 36(3): 400–413.
10.
GouZGChangJZhaiWYWangJY. Study on the self-setting property and the in vitro bioactivity of beta-Ca2SiO4. J Biomed Mater Res B2005; 73B(2): 244–251.
11.
TaylorHFW. Cement chemistry, 2nd ed. London, UK: Thomas Telford, 1997.
12.
BlackLBreenCYarwoodJDengCSPhippsJMaitlandG. Hydration of tricalcium aluminate (C(3)A) in the presence and absence of gypsum–studied by Raman spectroscopy and X-ray diffraction. J Mater Chem2006; 16(13): 1263–1272.
BrevalE. C3A hydration. Cem Concr Res1976; 6(1): 129–137.
18.
BrevalE. Gas-phase and liquid-phase hydration of C3A. Cem Concr Res1977; 7(3): 297–303.
19.
SantosADAraújoEBYukimituKBarbosaJCMoraesJCS. Setting time and thermal expansion of two endodontic cements. Oral Surg Oral Med Oral Pathol Oral Radiol Endod2008; 106(3): 77–79.
20.
DammaschkeTGerthHUVZuchnerHSchaferE. Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements. Dent Mater2005; 21(8): 731–738.
21.
DunneNJOrrJFMushipeMTEveleighRJ. The relationship between porosity and fatigue characteristics of bone cements. Biomaterials2003; 24(2): 239–245.
22.
FlemingGJPFarooqAABarraletJE. Influence of powder/liquid mixing ratio on the performance of a restorative glass-ionomer dental cement. Biomaterials2003; 24(23): 4173–4179.
23.
KokuboTTakadamaH. How useful is SBF in predicting in vivo bone bioactivity?. Biomaterials2006; 27(15): 2907–2915.
24.
XuSLinKWangZChangJWangLLuJ. Reconstruction of calvarial defect of rabbits using porous calcium silicate bioactive ceramics. Biomaterial2008; 29(17): 2588–2596.
25.
HenchLLPaschallHA. Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. J Biomed Mater Res B1973; 7(3): 25–42.
26.
SarkarNKCaicedoRRitwikPMoiseyevaRKawashimaI. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod2005; 31(2): 97–100.
27.
GandolfiMGCiapettiGTaddeiPPerutFTintiACardosoMV. Apatite formation on bioactive calcium-silicate cements for dentistry affects surface topography and human marrow stroamal cells proliferation. Dent Mater2010; 26(10): 974–992.
28.
GandolfiMGTaddeiPTintiAPratiC. Apatite-forming ability (bioactivity) of ProRoot MTA. Int Endod J2010; 43(10): 917–929.
29.
ChenCCHoCCChenCHDDingSJ. Physicochemical properties of calcium silicate cements for endodontic treatment. J Endod2009; 35(9): 1288–1291.
30.
ChenCCHoCCChenCHDWangWCDingSJ. In vitro bioactivity and biocompatibility of dicalcium silicate cements for endodontic use. J Endod2009; 35(11): 1554–1557.
31.
ChenCLHuangTHDingSJShieMYKaoCT. Comparison of calcium and silicate cement and mineral trioxide aggregate biologic effects and bone markers expression in MG63 cells. J Endod2009; 35(5): 682–685.
32.
WangXHSunHCChangJ. Characterization of Ca3SiO5/CaCl2 composite cement for dental application. Dent Mater2008; 24(1): 74–82.
33.
LaurentPCampsJDe MeoMDejouJAboutaI. Induction of specific cell responses to a Ca3SiO5-based posterior restorative material. Dent Mater2008; 24(11): 1486–1494.
34.
XynosIDEdgarAJButteryLDKHenchLLPolakJM. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass (R) 45S5 dissolution. J Biomed Mater Res2001; 55(2): 151–157.
35.
ValerioPPereiraMMGoesAMLeiteMF. The effect of ionic products from bioactive glass dissolution on osteoblast proliferation and collagen production. Biomaterials2004; 25(15): 2941–2948.
36.
HuanZGChangJ. Novel bioactive composite bone cements based on the beta-tricalcium phosphate-monocalcium phosphate monohydrate composite cement system. Acta Biomater2009; 5(4): 1253–1264.
37.
ReffittDMOgstonNJugdaohsinghRCheungHFJEvansBAJThompsonRPH. Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. Bone2003; 2(2): 127–135.
38.
SunHWuCDaiKChangJTangT. Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics. Biomaterials2006; 27(33): 5651–5657.
