Sage Journals: Discover world-class research

Abstract

Collagen derived from chum salmon (Oncorhynchus keta) was crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) during collagen fibrillogenesis and applied to an in vitro cell culture to evaluate its potential use as a scaffold for vascular tissue engineering. Human umbilical vein endothelial cells (HUVEC) were cultured on the crosslinked salmon collagen fibrillar gel (EDC-SC gel), and their growth rates and production levels of cytokines, including platelet-derived growth factor-BB and von Willebrand factor, were measured. Comparison was also made with bovine collagen gel crosslinked with EDC (EDC-BC gel). The growth and cytokine production of the HUVEC cultured on the EDC-SC gel were higher than those on the EDC-BC gel. In addition, HUVEC were found to attach to the EDC-BC gel through α2β1 integrin for native collagen, whereas they attached to the EDC-SC gel through αvβ3 integrin for denatured collagen as well as the α2β1 integrin, indicating that HUVEC recognized denatured domains in the EDC-SC gel. In conclusion, the EDC-SC gel can be used as a scaffold to support HUVEC growth, although the integrin-mediated attachment manner differs between the two gels.

Keywords

collagen salmon scaffold endothelial cells integrin tissue engineering.

Get full access to this article

View all access options for this article.

References

Lee, C.H. , Singla, A. and Lee, Y. (2001). Biomedical Applications of Collagen, Int. J. Pharm., 221(1-2): 1-22.

Akhouayri, O. , Lafage-Proust, M.H. , Rattner, A. , Laroche, N. , Caillot-Augusseau , A. , Alexandre, C. and Vico, L. (1999). Effects of Static or Dynamic Mechanical Stresses on Osteoblast Phenotype Expression in Three-dimensional Contractile Collagen Gels, J. Cell. Biochem. , 76(2): 217-230.

Feng, Z. , Yamato, M. , Akutsu, T. , Nakamura, T. , Okano, T. and Umezu, M. (2003). Investigation on the Mechanical Properties of Contracted Collagen Gels as a Scaffold for Tissue Engineering, Artif. Organs, 27(1): 84-91.

Iwasa, J. , Ochi, M. , Uchio, Y. , Katsube, K. , Adachi, N. and Kawasaki, K. (2003). Effects of Cell Density on Proliferation and Matrix Synthesis of Chondrocytes Embedded in Atelocollagen Gel, Artif. Organs, 27(3): 249-255.

Von den Hoff, J.W. (2003). Effects of Mechanical Tension on Matrix Degradation by Human Periodontal Ligament Cells Cultured in Collagen Gels, J. Periodontal Res., 38(5): 449-457.

Swatschek, D. , Schatton, W. , Kellermann, J. , Muller, W.E. and Kreuter, J. (2002). Marine Sponge Collagen: Isolation, Characterization and Effects on the Skin Parameters Surface-pH, Moisture and Sebum, Eur. J. Pharm. Biopharm., 53(1): 107-113.

Burjanadze, T.V. (2000). New Analysis of the Phylogenetic Change of Collagen Thermostability, Biopolymers , 53(6): 523-528.

Yunoki, S. , Nagai, N. , Suzuki, T. and Munekata, M. (2004). Novel Biomaterial from Reinforced Salmon Collagen Gel Prepared by Fibril Formation and Cross-linking, J. Biosci. Bioeng., 98(1): 40-47.

Nagai, N. , Mori, K. , Satoh, Y. , Takahashi, N. , Yunoki, S. , Tajima, K. and Munekata, M. (2007). In Vitro Growth and Differentiated Activities of Human Periodontal Ligament Fibroblasts Cultured on Salmon Collagen Gel , J. Biomed. Mater. Res. A., 82(2): 395-402.

10.

Baldus, S. , Koster, R. , Reimers, J. , Kahler, J. , Meinertz, T. and Hamm, C.W. (2001). Membrane-covered Stents: a New Treatment Strategy for Saphenous Vein Graft Lesions, Catheter Cardiovasc. Interv. , 53(1): 1-4.

11.

Cloft, H.J. , Kallmes, D.F. , Lin, H.B. , Li, S.T. , Marx, W.F. , Hudson, S.B. , Helm, G.A. , Lopes, M.B. , McGraw, J.K. , Dion, J.E. and Jensen, M.E. (2000). Bovine Type I Collagen as an Endovascular Stent-graft Material: Biocompatibility Study in Rabbits , Radiology, 214(2): 557-562.

12.

McKenna, C.J. , Camrud, A.R. , Sangiorgi, G. , Kwon, H.M. , Edwards, W.D. , Holmes Jr, D.R. and Schwartz, R.S. (1998). Fibrin-film Stenting in a Porcine Coronary Injury Model: Efficacy and Safety Compared with Uncoated Stents, J. Am. Coll. Cardiol., 31(6): 1434-1438.

13.

Nagai, N. , Yunoki, S. , Suzuki, T. , Sakata, M. , Tajima, K. and Munekata, M. (2004). Application of Cross-linked Salmon Atelocollagen to the Scaffold of Human Periodontal Ligament Cells, J. Biosci. Bioeng., 97(6): 389-394.

14.

Clark, E.A. and Brugge, J.S. (1995). Integrins and Signal Transduction Pathways: the Road Taken, Science, 268(5208): 233-239.

15.

Heino, J. (2000). The Collagen Receptor Integrins Have Distinct Ligand Recognition and Signaling Functions, Matrix Biol., 19(4): 319-323.

16.

Davis, G.E. (1992). Affinity of Integrins for Damaged Extracellular Matrix: Alpha V Veta 3 Binds to Denatured Collagen Type I through RGD Sites, Biochem. Biophys. Res. Commun., 182(3): 1025-1031.

17.

Wary, K.K. , Mainiero, F. , Isakoff, S.J. , Marcantonio, E.E. and Giancotti, F.G. (1996). The Adaptor Protein Shc Couples a Class of Integrins to the Control of Cell Cycle Progression, Cell, 87(4): 733-743.

18.

Giancotti, F.G. (1997). Integrin Signaling: Specificity and Control of Cell Survival and Cell Cycle Progression, Curr . Opin. Cell Biol., 9(5): 691-700.

19.

Vogel, W.F. , Abdulhussein, R. and Ford, C.E. (2006). Sensing Extracellular Matrix: an Update on Discoidin Domain Receptor Function, Cell Signal., 18(8): 1108-1116.

20.

Millette, E. , Rauch, B.H. , Kenagy, R.D. , Daum, G. and Clowes, A.W. (2006). Platelet-derived Growth Factor-BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells, Trends Cardiovasc. Med., 16(1): 25-28.

Biological Properties of Crosslinked Salmon Collagen Fibrillar Gel as a Scaffold for Human Umbilical Vein Endothelial Cells

Abstract

Keywords

Get full access to this article

References