Effect of Platelet-rich Plasma on the in vitro Proliferation and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Distinct Calcium Phosphate Scaffolds: The Specific Surface Area Makes a Difference

Abstract

The in vitro effect of platelet-rich plasma (PRP) on cell loading, proliferation, and osteogenic differentiation of human mesenchymal stem cells (MSC) is assessed on distinct resorbable and synthetic calcium phosphate scaffolds. A high specific surface area scaffold composed of calcium-deficient hydroxyapatite (CDHA; 48m²/g) is compared with one made out of β-tricalcium phosphate (β-TCP; surface area <0.5 m²/g). Fivefold concentrated fresh PRP is applied to scaffolds loaded with 2 × 10⁵ MSC (n = 5). These constructs are kept in a medium with osteogenic supplements for 3 weeks. The addition of PRP leads to a higher cell loading efficiency of MSC on CDHA (p = 0.0001), that reaches the values of β-TCP. Proliferation over 21 days is improved by PRP both on CDHA (p = 0.0001) and β-TCP (p = 0.014) compared to MSC/calcium phosphate composites. Without the addition of PRP, CDHA has a lower cell loading efficiency (p= 0.0001) and proliferation (p= 0.001) than β-TCP. The ALP activity is higher in the MSC/ceramics groups than in the monolayer controls (p<0.05). The addition of PRP does not significantly affect ALP activity. However, ALP activity varies considerably within the cell donors and different PRP-pools (p = 0.001), while the cell numbers do not vary within these two parameters. PRP generates a positive effect on the loading efficiency of MSC on the high specific surface scaffold CDHA that thereby reaches the loading efficiency of β-TCP. PRP improved proliferation, but its osteogenic properties on both calcium phosphate scaffolds are weak.

Keywords

platelet rich plasma mesenchymal stem cells calcium phosphate scaffolds calcium-deficient hydroxyapatite β-tricalcium phosphate tissue engineering bone regeneration bone healing.

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