To obtain immunocompatibility, graded zirconia—hydroxyapatite (ZrO 2-HA) composite bioceramic and simplex ZrO2-HA composite bioceramic are prepared. Peripheral blood mononuclear cells (PBMCs) harvested from healthy individuals are cultured with the two ceramic extracts to assess their effect on the transformation of lymphocytes, apoptotic ratio, CD69 expression, and expression of cytokine of TNFα and IL-6, with or without phytohemoagglutinin (PHA)-stimulated cells. Ceramic extracts did not activate the resting lymphocytes, whereas the response of the PHA-stimulated cells was significantly modified. The PBMCs activated by graded ZrO2-HA composite bioceramic is noticeably smaller than that by simplex ZrO2-HA composite bioceramic; these results, however can only be seen under the amplified effect of PHA-stimulation.
Kumar, P. , Oka, M. , Ikeuchi, K. , Shimizu, K. , Yamamur, O.T. , Okumura, H. and Kotoura, Y. (1991). Low Wear Rate of UHMWPE Against Zirconia Ceramic (Y-PSZ) in Comparison to Alumina Ceramic and SUS 316L Alloy , J. Biomed. Mater. Res., 25(2): 813—828 .
2.
Willmann, G. , Fruh, H.J. and Pfaff, H.G. (1996). Wear Characteristics of Sliding Pairs of Zirconia (Y-TZP) for Hip Endoprostheses , Biomaterials,17(22): 2157—2162 .
3.
Ben, A.A. and Treheux, T. (1991). Friction and Wear of Ultra High Molecular Weight Polyethylene Against Various New Ceramics , Wear, 142(1): 43—56 .
4.
Covacci, V. , Bruzzese, N. , Maccauro, G. , Andreassi, C. , Ricci, G.A. , Piconi, C. , Marmo, E. , Burger, W. and Cittadini, A. (1999). In vitro Evaluation of the Mutagenic and Carcinogenic Power of High Purity Zirconia Ceramic , Biomaterials, 20(4): 371—376 .
5.
Richter, H.G. , Burger, W. and Willmann, G. (1995). Ceramic Hip Joint Heads Made from Alumina and Zirconia—A Comparison , Ceram. Trans., 48: 73—81 .
6.
Christel, P. , Meunier, A. , Heller, M. , Torre, J.P. and Peille, C.N. (1989). Mechanical Prosperities and Short Time in vivo Evaluation of Yttrium Oxide Partially Stabilized Zirconia , J. Biomed. Mater. Res., 23(1): 45—61 .
7.
Li, J. , Liu, Y. , Hermansson, L. and So, R. (1993). Evaluation of Biocompatibility of Various Ceramic Powders with Human Fibroblasts in vitro , Clin. Mater., 12(4): 197—201 .
8.
Ito, A. , Tateishi, T. , Niwa, S. and Tange, S. (1993). In vitro Evaluation of the Cytocompatibility of Wear Particles Generated by UHMWPE Zirconia Friction , Clin. Mater., 12(4): 203—209 .
9.
Ramachandra, R.R. and Kannan, T.S. (2002). Synthesis and Sintering of Hydroxyapatite—Zirconia Composites , Mater. Sci. Eng. , 20(1—2): 187—193 .
10.
Guo, H. , Khorx, K.A. , Boey, Y.C. and Miao, X. (2003). Laminated and Functionally Graded Hydroxyapatite/Yttria Stabilized Tetragonal Zirconia Composites Fabricated by Spark Plasma Sintering , Biomaterials, 24(4): 667—675 .
11.
Li, W. and Gao, L. (2003). Fabrication of HAp—ZrO2 (3Y) Nano-composite by SPS , Biomaterials, 24(6): 937—940 .
12.
Zhitomirsky, I. (2000). Electrophoretic Hydroxyapatite Coatings and Fibers , Materials Letters, 42(4): 262—271 .
13.
Gledhill, H.C. , Turner, I.G. and Doyle, C. (1999). Direct Morphological Comparison of Vacuum Plasma Sprayed and Detonation Gun Sprayed Hydroxyapatite Coatings for Orthopaedic Applications , Biomaterials, 20(4): 315—322 .
14.
Cheang, P. and Khor, K.A. (1996). Addressing Processing Problems Associated with Plasma Spraying of Hydroxyapatite Coatings , Biomaterials, 17(5): 537—544 .
15.
Kumar, R.R. , Yang, X. , Ban, S. and Maruno, S. (1997). Proc. IMMM, Mie University Press, Tsu, Japan, pp. 618—623.
16.
Wooley, P.H. , Petersen, S. , Song, Z. and Nasser, S. (1997). Cellular Immune Responses to Orthopaedic Implant Materials Following Cemented Total Joint Replacement , J. Orthop. Res., 15(6): 874—880 .
17.
Goodman, S.B. , Huie, P. , Song, Y. , Lee, K. , Doshi, A. , Rushdieh, B. , Woolson, S. , Maloney, W. , Schurman, D. and Sibley, R. (1997). Loosening and Osteolysis of Cemented Joint Arthroplasties, A Biologic Spectrum , Clin. Orthop., 337: 149—163 .
18.
Nagarajan, V.S. and Rao, K.J. (1993). Structural, Mechanical and Biocompatibility Studies of Hydroxyapatite-derived Composites Toughened by Zirconia Addition , J. Mater. Chem., 3(1): 43—51 .
19.
Wu, J.-M. and Yeh, T.-S. (1998). Sintering of Hydroxylapatite-zirconia Composite Materials, Journal of MaterialsScience, 23(10): 3771—3777 .
20.
Granchi, D. , Ciapetti, G. , Savarino, L. , Cenni, E. , Pizzoferralo, A. , Baldini, N. and Giunti, A. (2002). Effects of Bone Cement Extracts on the Cell-mediated Immune Response , Biomaterials , 23(4): 1033—1041 .
21.
FDAF-Center for Devices and Radiological Health : Guidance for Industry and FDA Reviewers. Immunotoxicity Testing Guidance. May 6, 1999. pp. 1—16.
22.
Ciapetti, G. , Granchi, D. , Stea, S. , Cervellati, M. , Pizzoferrato, A. and Toni, A. (2000). In vitro Testing of Ten Bone Cements after Different Time Intervals from Polymerization , J. Biomed. Sci. Polym. Ed.11(5): 481—493 .
23.
Wang, M.L. , Tuli, R. , Manner, P.A. , Sharkey, P.F. , Hall, D.J. and Tuna, R.S. (2003). Direct and Indirect Induction of Apoptosis in Human Mesenchymal Stem Cells in Response to Titanium Particles , J. Orthop. Res., 21(4): 697—707 .
24.
Lanier, L.L. , Buck, D.W. , Rhodes, L. , Ding, A. , Evans, E. , Barney, C. and Phillips, J.H. (1988). Interleukin 2 Activation of Natural Killer Cells Rapidly Induces the Expression and the Phosphorylation of the Leu-23 Antigen , J. Exp. Med., 167(5): 1572—1585 .
25.
Mardiney III, M. , Brown, M.R. and Fleisher, T.A. (1996). Measurement of T-cell CD69 Expression: A Rapid and Efficient Means to Assess Mitogenor Antigen-induced Proliferative Capacity in Normals , Cytometry, 26(4): 305—310 .
26.
Blanka, R. (2000). Immunocompatibility and Biocompatibility of Cell Delivery Systems , Advanced Drug Delivery Reviews, 42(1—2): 65—80 .
27.
Hirano, T. , Akira, S. , Taga, T. and Kishimoto, T. (1990). Biological and Clinical Aspects of Interleukin 6 , Immunol. Today, 11(12): 443—449 .
28.
Abbas, A.K. , Lichtman, A.H. and Pober, J.S. (1997). Cellular and Molecular Immunology, W.B. Saunders, PA .
29.
Rıhova, B. (1996). Biocompatibility of Biomaterials: Hemocompatibility, Immunocompatibility and Biocompatibility of Solid Polymeric Materials and Soluble Targetable polymeric Carriers , Adv. Drug Deliv. Rev., 21(2): 157—176 .
30.
Schindler, R. , Lonnemann, G. , Shaldon, S. , Koch, K.M. and Dinarello , C.A. (1990). Transcription, not Synthesis, of Interleukin-1 and Tumor Necrosis Factor by Complement , Kindy Int., 37(1): 85—94 .
