Biomaterials in Periodontal Regenerative Surgery: Effects of Cryopreserved Bone,Commercially Available Coral,Demineralized Freeze-dried Dentin,and Cementum on Periodontal Ligament Fibroblasts and Osteoblasts
Restricted accessOtherFirst published online October, 2004
Biomaterials in Periodontal Regenerative Surgery: Effects of Cryopreserved Bone,Commercially Available Coral,Demineralized Freeze-dried Dentin,and Cementum on Periodontal Ligament Fibroblasts and Osteoblasts
The ultimate goal of periodontal therapy is to achieve successful periodontal regeneration. The effects of different biomaterials, allogenic and alloplastic, used in periodontal surgeries to achieve regeneration have been studied in vitro on periodontal ligament (PDL) cells and MC3T3-E1 cells. The materials tested included cryopreserved bone allograft (CBA), coralline hydroxyapatite (CH), demineralized freeze-dried dentin (DFDD), and cementum. CBA and CH revealed an increase in initial PDL cell attachment, whereas CH resulted in an increase in long-term PDL cell attachment. Mineral-like nodule formation was observed significantly higher in DFDD compared to other materials tested for osteoblasts. Based on the results of this in vitro study, we conclude that the materials used are all biocompatible with human PDL cells and osteoblasts, which have pivotal importance in periodontal regeneration.
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References
1.
1. Karring, T., Nyman, S., Gottlow, J. and Laurell, L. (1993). Development of the Biological Concept of Guided Tissue Regeneration - Animal and Human Studies , Periodontol. 2000, 1: 26-35 .
2.
2. Zaman, K.U., Sugaya, T., Hongo, O. and Kato, H. (2000). A Study of Attached and Oriented Human Periodontal Ligament Cells to Periodontally Diseased Cementum and Dentin after Demineralizing with Neutral and Low pH Etching Solution , J. Periodontol., 71: 1094-1099 .
3.
3. Mellonig, J.T. (1992). Autogenous and Allogeneic Bone Grafts in Periodontal Therapy , Crit. Rev. Oral. Biol. Med., 3: 333-352 .
4.
4. Needleman, I., Tucker, R., Giedrys-Leeper, E. and Worthington, H.A. (2002). Systematic Review of Guided Tissue Regeneration for Periodontal Infrabony Defects , J. Periodontal. Res., 37: 380-388 .
5.
5. Nkenke, E., Schultze-Mosgau, S., Radespiel-Troger, M., Kloss, F. and Neukam, F.W. (2001). Morbidity of Harvesting of Chin Grafts: A Prospective Study , Clin. Oral Implants Res., 12: 495-502 .
6.
6. Borghetti, A., Novakovitch, G., Louise, F., Simeone, D. and Fourel, J. (1993). Cryopreserved Cancellous Bone Allograft in Periodontal Intraosseous Defects , J. Periodontol., 64: 128-132 .
7.
7. Yukna, R.A. and Yukna, C.N. (1998). A 5-year Follow-up of 16 Patients Treated with Coralline Calcium Carbonate (BIOCORAL) Bone Replacement Grafts in Infrabony Defects , J. Clin. Periodontol., 25: 1036-1040 .
8.
8. Morris, M.L. (1975). The Implantation of Decalcified Human Dentin and Cementum and Freshly Devitalized Autogenous Bone into the Subcutaneous Tissues of the Rat , J. Periodontol., 46: 284-287 .
9.
9. Rompen, E.H., Kohl, J., Nusgens, B. and Lapiere, C.M. (1993). Kinetic Aspects of Gingival and Periodontal Ligament Fibroblast Attachment to Surface-conditioned Dentin , J. Dent. Res., 72: 607-612 .
10.
10. Somerman, M.J., Mera-Perez, M., Merkhofer, R.M. and Foster, R.A. (1987). In vitro Evaluation of Extracts of Mineralized Tissues for their Application in Attachment of Fibrous Tissue , J. Periodontol., 58: 349-351 .
11.
11. Pituru, S., Suvion, N., Hekmati, H., Olsen, S. and Narayanan, S.A. (1992). Binding of a Cementum Attachment Protein to Extracellular Matrix Components and to Dental Surfaces , J. Periodontal Res., 27: 640-646 .
12.
12. White, J.M., Goodis, H.E., Marshall, S.J. and Marshall, G.W. (1994). Sterilization of Teeth by Gamma Radiation , J. Dent. Res., 73: 1560-1567 .
13.
13. Somerman, M.J., Archer, S.Y., Imm, G.R. and Foster, R.A. (1988). A Comparative Study of Human Periodontal Ligament Cells and Gingival Fibroblasts in vitro , J. Dent. Res., 67: 66-70 .
14.
14. Nohutcu, R.M., Somermen, M.J. and McCauley, L.K. (1995). Dexamethasone Enhances the Effects of Parathyroid Hormone on Human Periodontal Ligament Cells in vitro , Calcif. Tissue Int., 56: 571 .
15.
15. Wahl, R., Geissler, W. and Maasch, H.J. (1985). Comparison of Classical Lowry, Modified Lowry and a Dye-binding Assay for the Estimation of Protein in Allergen Extracts and Influence of Different Parameters on the Modified Lowry Assay , Biol. Chem. Hoppe Seyler, 366: 979-984 .
16.
16. McCauley, L.K., Tözüm, T.F., Kozloff, K.M., Koh-Paige, A.J., Chen, C., Demashkieh, M., Cronovich, H., Richard, V., Keller, E.T., Rosol, T.J. and Goldstein, S.A. (2003). Transgenic Models of Metabolic Bone Disease: Impact of Estrogen Receptor Deficiency on Skeletal Metabolism , Conn. Tissue Res., 44: 250-263 .
17.
17. Nohutcu, R.M., McCauley, L.K., Koh, A.J. and Somerman, M.J. (1997). Expression of Extracellular Matrix Proteins in Human Periodontal Ligament Cells during Mineralization in vitro , J. Periodontol., 68: 320-327 .
18.
18. Bowers, G.M., Chadroff, B., Carnevale, R., Mellonig, J., Corio, R., Emerson, J., Stevens, M. and Romberg, E. (1989). Histologic Evaluation of New Attachment Apparatus Formation in Humans. Part I ., J. Periodontol., 60: 664-674 .
19.
19. Goldberg, V.M. (2000). Selection of Bone Grafts for Revision Total Hip Arthroplasty , Clin. Orthop., 381: 68-76 .
20.
20. Actis, A.B., Obwegeser, J.A. and Ruperez, C. (2002). Volume Variations of Bone Tissue after Undergoing Different Physical and Chemical Treatments , J. Biomater. Appl., 17: 71-81 .
21.
21. Oh, J.H., Zoller, J.E. and Kubler, A. (2002). A New Bone Banking Technique to Maintain Osteoblast Viability in Frozen Human Iliac Cancellous Bone , Cryobiology, 44: 279-287 .
22.
22. Doherty, M.J., Schlag, G., Schwarz, N., Mollan, R.A., Nolan, P.C. and Wilson, D.J. (1994). Biocompatibility of Xenogeneic Bone, Commercially Available Coral, a Bioceramic and Tissue Sealant for Human Osteoblasts , Biomaterials, 15: 601-608 .
23.
23. Sautier, J.M., Nefussi, J.R., Boulekbache, H. and Forest, N. (1990). In vitro Bone Formation on Coral Granules , In Vitro Cell Dev. Biol., 26: 1079-1085 .
24.
24. Begley, C.T., Doherty, M.J., Hankey, D.P. and Wilson, D.J. (1993). The Culture of Human Osteoblasts Growth upon Bone Graft Substitutes , Bone, 14: 661-667 .
25.
25. Zambonin, G. and Grano, M. (1995). Biomaterials in Orthopaedic Surgery: Effects of Different Hydroxyapatites and Demineralized Bone Matrix on Proliferation Rate and Bone Matrix Sythesis by Human Osteoblasts , Biomaterials, 16: 397-402 .
26.
26. Somerman, M.J., Foster, R.A., Imm, G.M., Sauk, J.J. and Archer, S.Y. (1989). Periodontal Ligament Cells and Gingival Fibroblasts Respond Differently to Attachment Factors in vitro , J. Periodontol., 60: 73-77 .
27.
27. Fontaine, A.R. and Hall, B.D. (1981). Biocompatibility of Echinoderm Skeleton with Mammalian Cells in vitro: Preliminary Evidence , J. Biomed. Mater. Res., 15: 61-71 .
28.
28. Morris, M.L. (1971). The Implantation of Decalcified Human Dentin and Cementum with Autogenous Bone and Marrow into Subcutaneous Tissues of Rat , J. Periodontol., 42: 57-731-61-735 .
29.
29. Register, A.A., Scopp, I.W., Kassounny, D.Y., Dfau, F.R. and Peskin, D. (1972). Human Bone Induction by Allogenic Dentin Matrix , J. Periodontol., 43: 459-467 .
30.
30. Fugazzotto, P.A., De Paoli, S. and Benfenati, S.P. (1986). The Use of Allogenic Freeze-dried Dentin in the Repair of Periodontal Osseous Defects in Humans , Quintessence Int., 17: 461-477 .
