Surface Functionalization of Polystyrene Nanoparticles with Liposaccharide Monomers: Preparation,Characterization and Applications

Abstract

Latex particles bearing carbohydrate species were prepared by emulsion copolymerization of styrene or methyl methacrylate with polymerizable liposaccharide surfactants; 11-(N-p-vinylbenzyl) amido undecanoyl maltobionamide (LIMA), 6-(2-methylpropenoyloxy)hexyl β-d-cellobioside (CHMA) and 10-(2-methylpropenoyloxy)decyl β-d-cellobiouronoside (CDMA). Due to their amphiphilic structure, these three monomers exhibit surface active properties in dilute solutions and mesomorphic properties in concentrated solutions. Their incorporation onto latex particles was performed according to a batch process or a seed polymerization process. The presence of the carbohydrate residues at the particle surface was directly observed by NMR. The potential application of these functionalized latexes in the biomedical diagnostic field was evaluated by studying the adsorption of bovine serum albumin, and by performing covalent binding of antibodies and single-stranded DNA fragments on their surface.

Get full access to this article

View all access options for this article.

References

b)- Arshady, R. 1993, Biomaterials, 14, no.1,5.

2. Laverriere, A. C. 1990. PhD Thesis, Lyon, France.

3. Charleux, B. , Pichot, C. , and Fanget, P. 1992. Makromol. Chem., 193, 205.

4. Delair, T. , Pichot, C. , and Mandrand, B. 1991. Colloid Polym. Sci., 272, 72.

5. Delair, T. , Marguet, V. , Pichot, C. , and Mandrand, B. 1994. Colloid Polym. Sci., 272, 962.

d)- Wulff, G. , Schmid, J. , and Venhoff, T. , 1996. Macromol. Chem. Phys., 197, 259.

7. Davies, R. C. , Lynn, R. A. R. , Davis, S. S. , Hearn, J. , Watts, J. F. , Vickerman, J. C. , and Paul A. J. 1993, Langmuir, 9, 1637.

8. Sugiyama, K. and Oku, T. 1995. Polymer J., 27, no. 2, 179.

9. Charreyre, M.-T. , Boullanger, P. , Delair, T. , Mandrand, B. , and Pichot, C. 1993. Colloid Polym. Sci. 271, 668.

10.

10. Revilla, J. , Elaïssari, A. , Pichot, C. , and Gallot, B. 1995. Polymers for Adv. Tech., 6, 455.

11.

11. Demharter, S. , Richtering, W. , and Mulhaupt R. 1995. Polymer Bulletin, 34, 271.

12.

12. Charreyre, M.-T. 1992. PhD Thesis, Lyon, France.

13.

13. Revilla, J. , Pichot, C. , Elaïssari, A. , and Gallot, B. 1996. Polymer, 37, no. 4, 667.

14.

14. Betton, F. , Theretz, A. , Elaïssari, A. and Pichot, C. 1993. Colloids Surfaces, 1, 97.

15.

15. Bradford, M. M. 1976. Anal. Biochem., 72, 248.

16.

16. Changhong, Y. , Xianming, Z. , and Zonghua, S. 1990. J. Applied Polym. Sci., 40, 89.

17.

17. Cochin, D. , Zana, R. , and Candau, F. 1993. Polymer International, 30, 491.

18.

18. Gallot, B. , Revilla, J. , Charreyre, M.-T. , and Pichot, C. 1994. Liquid Cristal-line Polymers, C. Carfagna (Ed.), Pergamon, 1.

19.

19. Graillat, C. , Dumont, B. , Depraetere, P. , Vintenon, V. , and Pichot, C. 1991. Langmuir, 7, 872.

20.

20. Norde, W. and Lyklema, J. 1989. Colloids and surfaces, 38, 1.

21.

21. Young, B. R. , Pitt, W. G. , and Cooper, S. L. 1988. J. Colloid Interface Sci., 124, 28.

22.

22. Kondo, A. , Oku, S. , and Higashitani, K. 1991. J. Colloid Interface Sci., 143, 214.

23.

23. Andrade, J. D. 1985. Protein Adsorption, Surface and Interfacial Aspects of Biomedical Polymers, J. D. Andrade (Ed.), Vol. 2, Plenum, New York.

24.

24. Suzawa, T. and Shirahama, H. 1991. Adv. Colloid Interf. Sci., 35, 139.

25.

25. Revilla, J. , Elaïssari, A. , Carriere, P. , and Pichot, C. 1996. J. Colloid Interf. Sci., 180, 405.

26.

26. Elgersma, A. V. , Zsom, R. J. , Norde, W. , and Lyklema, L. J. 1990. J. Colloid Interf. Sci., 138, 145.

27.

27. Kondo, A. and Higashitani, K. 1992. J. Colloid Interf. Sci., 150, 344.