Cell Growth on Immobilized Cell-Growth Factor. Interactions of Fibroblast Cells with Insulin Immobilized on 2-Hydroxyethyl Methacrylate /Ethyl Methacrylate Copolymer Membranes

Abstract

The adhesion and growth of mouse fibroblast cells STO were in vestigated in the presence of 2-hydroxyethyl methacrylate/ethyl methacrylate copolymer membranes with immobilized insulin. For the copolymer membrane without immobilized insulin, cell adhesion and growth were moderately sup pressed, on those copolymer membranes containing very high or very low 2-hydroxyethyl methacrylate content. A similar effect by the copolymer compo sition was observed on the membranes immobilized with small amounts of in sulin. Large amounts of immobilized insulin remarkably enhanced cell growth and masked the copolymer composition effects. The immobilized insulin en hanced the cell growth more than free insulin.