Chitosan, a polycationic polymer and waste product from the sea food processing industry, is an abundant natural resource that has, as yet, not been fully utilized. Advantages of this polymer include availability, low cost, high biocompatibility, biodegradability and ease of chemical modification. In this paper, the physicochemical properties of chitosan, as well as its numerous applications, are reviewed with particular emphasis on its use in water treat ment, pharmaceutics, agriculture and membrane formation.
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References
1.
Allan, G.G., L.C. Altman, R.E. Bensinger, D.K. Ghosh, Y. Hirabayashi, A.N. Neogi and S. Neogi.1984. In Chitin, Chitosan and Related Enzymes , J. P. Zikakis, ed., Academic Press, Inc., pp. 119-133.
2.
Alper, J.1984. Science84, (March):36-43.
3.
Muzzarelli, R.A.A.1977. ChitinToronto: Pergamon of Canada Ltd.
4.
Rigby, G.W.1934. "Substantially Undergraded Deacetylated Chitin and Process for Producing the Same," U.S. Patent 2,040,879.
5.
Rigby, G.W.1934. "Process for the Preparation of Films and Filaments and Products Thereof;' U.S. Patent 2,040,880.
6.
Clark, G.L. and A.F. Smith.1936. "X-ray Diffraction Studies of Chitin, Chitosan and Derivatives," J. Phys. Chem., 40:863-879.
7.
Bartnicki-Garcia, S. and E. Reyers.1968. Biochim. Biophys. Acta, 165(1):32-42.
8.
Hadwiger, L.A. and J.M. Backman.1980. Plant Physiol. , 66(2):205-211.
9.
Knorr, D.1984. Food Technology, (January):85-97.
10.
Brine, C.J.1984. Chapters 17-23 in Chitin, Chitosan and Related Enzymes , J. P. Zikakis, ed., Academic Press, Inc.
11.
Senstand, C. and B. Mattiasson.1989. Biotech. Bioeng. , 34:387-393.
12.
Senstand, C. and B. Mattiasson.1989. Biotech. Bioeng ., 33:216-220.
13.
Sirca, A.E. and R.J. Woodman.1971. J. Nat. Cancer Inst., 47(2):377-388.
14.
Allan, C.R. and L.A. Hadwiger.1979. Exp. Mycol., 3(3):285-287.
15.
Balassa, L.L. and J.F. Prudden.1978. MIT Sea Grant Rep. MITSG, 78-7, Proc. Int. Conf. Chitin/Chitosan, 1st PB 285 640, pp. 296-305.
16.
Malette, W.G. Jr., H.J. Quigley and E.D. Adiches.1986. In Chitin in Nature and Technology, R. Muzzarelli, C. Jeuniaux and G. W. GooDay, eds., New York: Plenum Press, pp. 435-442.
17.
Suzuki, K., Y. Ogawa, K. Hashimoto, S. Suzuki and M. Suzuki.1984. Microbiol. Immunol., 28:903-912.
18.
Nishimura, K. , S. Nishimura, N. Nishi, S. Tokura and I. Azuma.1986. In Chitin in Nature and Technology, R. Muzzarelli , C. Jeuniaux and G. W. GooDay , eds., New York: Plenum Press , pp. 477-483.
19.
Eida, T. and H. Hidaka.1988. Jpn. Fudo Saiensu, 27(12):56-63.
20.
Yano, S. and T. Tsugita.1988. "Chitosan-Containing Seed Coatings for Yield Enhancement," Jpn. Kokai Tokkyo Koho JP 63,139,102 [88,139,102].
Hirano, S., K. Tobetto, M. Hasegawa and N. Matsuda.1980. J. Biomedical Materials Research, 14:477-486.
23.
Rha, C., D. Rodriguez-Sanchez and C. Kienzle-Sterzer .1984. In Biotechnology of Marine Polysaccharides, R. R. Colwell, E. R. Pariser and A. J. Sinskey , eds., Washington: Hemisphere Publishing Corp., pp. 283-311.
24.
Knorr, D., M.D. Beaumont and Y. Pandya.1987. Biotechnol. Food Ind., Proc. Int. Symp. , pp. 389-400.
25.
Shiotani, T. and Y. Shiiki.1986. "Preparation of Capsules Using Phosphates and Chitin Derivatives," Jpn. Kokai Tokkyo Koho JP 61,153,135 [86,153,135].
26.
Smith, N.A. , M.F.A. Goosen, G.A. King, P. Faulkner and A.J. Daugulis.1988. Biotechnology Letters .
27.
McKnight, C.A. , A. Ku , M.F.A. Goosen, D. Sun and C. Penney.1987. " Synthesis of Chitosan-Alginate Microcapsule Membranes," J. Bioactive and Compatible Polymers , 3:334-355.
28.
Muzzarelli, R.A.A.1973. Natural Chelating Polymers . Toronto: Pergamon of Canada Ltd ., pp. 83-95.
29.
Kobayashi, T., Y. Takiguchi, K. Shimahara and T. Sannan.1988. Nippon Nogei Kagaku Kaishi, 62(10):1463-1469.
30.
Prasad, N. and C. Ramakrishnan .1972. Indian J. Pure Appl. Phys. , 10:501-505.
31.
Gardner, K.H. and J. Blackwell.1975. Biopolymers , 14:1581-1595.
32.
Averbach, B.L.1975. Report MITSG 75-17, NOAA 75102204, National Information Service, U. S. Department of Commerce.
33.
Kurita, K., T. Sannan and Y. Iwakura.1977. Makromol. Chem., 178:3197-3202.
34.
Bough, W.A., W.L. Salter, A.C.M. Wu and B.E. Perkins.1978. Biotech. Bioeng., 20:1931-1943.
35.
Muzzarelli, R.A.A. , F. Tanfani, G. Scarpini and G. Laterza.1980. J. Biochem. Biophys. Methods, 2:299-306.
36.
Domszy, J.G. and G.A.F. Roberts.1985. Macromol. Chem. , 186:1671-1677.
37.
Gummow, B.D. and G.A.F. Roberts.1985. Macromol. Chem., 186:1239-1244.
38.
Maghami, G.G. and G.A. Roberts.1988. Makromol. Chem., 189:2239-2243.
39.
Muzzarelli, R.A.A.1984. In New Development in Industrial Polysaccharides, V. Crescenzi and I. C. M. Dea, eds., New York: Gordon and Breach, pp. 417-450.
40.
Muzzarelli, R.A.A.1985. In The Polysaccharides , G. O. Aspinall, ed., London: Academic Press, Inc., 3:417-451.
41.
Takeda, K., K. Shimizu and M. Goto.1989. Manufacture of Low Molecular Weight Chitosan, Jpn. Kokai Tokkyo Koho JP 01,11,101 [89,11,101].
42.
Kushino, S. and Y. Orihara.1988. "Enzymatic Manufacture of Low Molecular-Weight Chitosan and Its Use in Pharmaceutical, Food, and Other Manufacture," Jpn. Kokai Tokkyo Koho JP 63,63,388 [88,63,388].
43.
Maghami, G.G. and G.A.F. Roberts.1988. Makromol. Chem., 189:195-200.
44.
Kienzle-Sterzer, C., D. Rodriguez-Sanchez and C. Rha.1984. In Chitin, Chitosan and Related Enzymes, J. P. Zikakis, ed., Academic Press, Inc., pp. 383-396.
Rinaudo, M. and A. Domaro.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sanford, eds., New York: Elsevier Applied Science, pp. 71-86.
47.
Kushino S. and H. Asano.1988. "Water-Soluble Chitosan," Jpn. Kokai Tokkyo Koho JP 63,225,602 [88,225,602].
48.
Tsezos, M.1983. Biotech. Bioeng., 25:2025-2040.
49.
Ogawa, K., K. Oka, T. Miyanishi and S. Hirano.1984. In Chitin, Chitosan and Related Enzymes , J. P. Zikakis, ed., Academic Press, Inc., pp. 327-346.
50.
Maruca, R., B.J. Suder and J.P. Wightmen.1982. J. Appl. Polym. Sci., 27:4827-4837.
51.
Kurita, K., T. Sannan and Y. Iwakura.1979. J. Appl. Polym. Sci., 23:511-515.
52.
Koyama, Y. and A. Taniguchi.1986. J. Applied Polymer Sci., 31:1951-1954.
53.
Kurita, K., Y. Koyama and A. Taniguchi.1986. J. Applied Polymer Sci., 31:1169-1176.
54.
Kurita, K., S. Chikaoka and Y. Koyama.1988. Chem. Letters, pp. 9-12.
55.
Muzzarelli, R.A.A. , F. Tanfani and G. Scarpini.1980. Biotech. Bioeng. , 22:885-896.
56.
Muzzarelli, R.A.A. , F. Tanfani, G. Scarpini and E. Tucci.1980. J. Applied Biochem ., 2:54-59.
57.
Lang, G. and T.C. Wella AG.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp. 139-147.
58.
Muzzarelli, R.A.A.1989. In Chitin and Chitosan , G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science , pp. 87-99.
59.
No, H.K. and S.P. Meyers.1989. J. Food Sci., 54(1):60-62.
60.
Wu, A.C.M. , W.A. Bough, M.R. Holmes and B.E. Perkins.1978. Biotech. Bioeng. , 20:1957-1968.
61.
No, H.K. and S.P. Meyers.1989. J. Agric. Food Chem. , 37(3):580-583.
62.
Bronner, R. , E. Westphal and F. Dreger.1989. Physiol. Mol. Plant Pathol., 34(2):117-130.
63.
Hadwiger, L.A. , B. Fristensky and R.C. Riggleman.1984. In Chitin, Chitosan, and Related Enzymes, J. P. Zikakis, ed., Academic Press, Inc., pp. 291-302.
64.
Horisberger, M. and M.F. Clerc.1989. Histochemistry , 90(3):165-175.
65.
Gualtieri, P., L. Barsanti and V. Passarelli.1988. Ann. Inst. Pasteur/Microbiol., 139(6):717-726.
66.
Shinagawa, K., G. Takemura and A. Kobayashi.1979. Janap Kokai Tokkyo Koho, Pat. 79,152,685.
67.
Seo, T., T. Kanbara and T. Iijima.1988. J. Appl. Polym. Sci., 36(6):1443-1451.
68.
Watanabe, I. and H. Bandai.1988. "Cleaning Agents for Bathtubs," Jpn. Kokai Tokkyo Koho JP 63,12,699 [88,12,699].
69.
Hirano, S.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York : Elsevier Applied Science, pp. 37-43.
70.
McCormick, D.1989. Bio/Technology, 5:27.
71.
Hirano, S., H. Seino, Y. Akiyama and I. Nonaka.1989. Polym. Mater. Sci. Eng. , 59:897-901.
72.
Muzzarelli, R.A.A. and O. Tubertini.1969. Talanta , 16:1571-1577.
73.
Masri, M.S. , F.W. Reuter and M.J. Friedman.1974. Applied Polymer Sci., 18:675-681.
74.
Hirano, S., H. Senda, Y. Yamamoto and A. Watanabe.1984. In Chitin, Chitosan, and Related Enzymes , J. P. Zikakis, ed., Academic Press, Inc., pp. 77-95.
75.
Nakajima, A. and K. Shinoda.1977. J. Appl. Pol., 21:1249.
76.
Uragami, T., F. Yoshida and M. Sugihara.1988. Sep. Sci. Technol., 23(10-11):1067-1082.
Tokunaga, M.1988. "Improvement of Colorfastness of Cellulosic Fibres and Their Blends Dyed with Direct and/or Reactive Dyes ;' Jpn. Kokai Tokkyo Koho JP 63,175,186 [88,175,186].
79.
Aizawa, Y. and T. Noda.1988. "Antistatic Photographic Paper," Jpn. Kokai Tokkyo Koho JP 63,189,859 [88,189,859].
80.
Kibune, K., Y. Yamaguchi, K. Motosugi.1988. "Manufacture of Bandages from Chitin Fibres," Jpn. Kokai Tokkyo Koho JP 63,209,661 [88,209,661].
81.
Motosugi, K. , Y. Yamaguchi and K. Kibune.1988. "Chitosan Sponges as Surgical Dressings," Jpn. Kokai Tokkyo Koho JP 63,90,507 [88,90,507].
82.
Tokura, S., M. Itoyama and S. Hiroshi.1988. "Partially Sulfated Chitosan Oligomers Immobilized on Chitosan for Antithrombogenic Medical Goods;' Jpn. Kokai Tokkyo Koho JP 63,89,167 [88,89,167].
83.
Uragami, T. , H. Mori and Y. Noishiki.1988. Jinko Zoki, 17(2):511-514.
84.
Chandy, T. and C.P. Sharma.1988. Polym. Sci. Technol., 38:297-311.
85.
Chandy, T. and C.P. Sharma.1989. J. Colloid Interface Sci., 130(2): 331-340.
86.
Suzuki, S., M. Suzuki and H. Katayama.1988. "Chitin and Chitosan Oligomers as Hypolipemics and Formulations Containing Them," Jpn. Kokai Tokkyo Koho JP 63,41,422 [88,41,422].
87.
Ouchi, T., T. Banba, T. Matsumoto, S. Suzuki and M. Suzuki.1989. J. Bioact. Comp. Polymers.
88.
Shibasaki, K., T. Matsukubo, N. Sugihara, E. Tashiro, Y. Tanabe and Y. Takaesu.1988. Koku Eisei Gakkai Zasshi, 38(4):572,573.
89.
Miyata, T., T. Yoneda and M. Izume.1988. "Manufacture of Eye Humour Fluid Substitutes with N-carboxyacylchitosan," Jpn. Kokai Tokkyo Koho JP 63,220,865 [88,220,865].
90.
Namita, K., N. Miyajima, M. Higo and J. Nakayama.1988. "Pharmaceuticals Containing Chitin, Chitosan, and Their Derivatives for Treatment of Bone Diseases," Jpn. Kokai Tokkyo Koho JP 63,156,726 [88,156,726].
91.
Collumbel, C. , O. Damour, C. Gagnieu, F. Poinsignon, C. Echinard and J. Marichy.1988. " Biomaterials for Artificial Skin and Implants Containing Acetylated Chitosan, Collagens, and Glycosaminoglycans," Eur. Pat. Appl. EP 296,078.
92.
Nagai, T., Y. Saway and N. Nambu.1984. In Chitin, Chitosan, and Related Enzymes , J. P. Zikakis, ed., Academic Press, Inc., pp. 21-40.
93.
Tian-Rui, T. , K. Inouye, Y. Machida, T. Sannan and T. Nagai.1988. 48(4):318-321.
94.
Bodmeier, R. , H. Chen and O. Paeratakul.1989. Pharm. Res., 6(5):413-417.
95.
Kanke, M., H. Katayama, S. Tsuzuki and H. Kuramoto.1989. Chem. Pharm. Bull., 37(2):523-525.
96.
Yabe, H., Y. Kawamura and I. Kurahashi.1988. "Coloured Cosmetic Bases Containing Fine Powders of Chitin and Chitosan," Jpn. Kokai Tokkyo Koho JP 63,161,001 [88,161,001].
97.
Lang, G., G. Maresch and H.R. Lenz.1989. "Nail Polishes Containing O-Benzyl-N-Hydroxyalkylchitosan as Film-Forming Component," Ger. Offen. DE 3,723,811.
98.
Matsumura, S., T. Karigome and K. Nomoto.1989. " Manufacture of N-Carboxymethoxycarbonylchitosan FoeMoisturizers," Jpn. Kokai Tokkyo Koho JP 01,14,203 [89,14,203].
99.
Lang, G. and H. Wendel.1988. "Hair and Skin Fixative Based on Chitosan and on Ampholytic Copolymer," Ger. Offen. DE 3,644,097.
100.
Lang, G. and H. Wendel.1988. "Quaternized Chitosan Derivatives, A Method for Their Preparation, and Cosmetics Containing Them," Eur. Pat. Appl. EP 290,741.
101.
Mizushima, M.1988. "Hair Preparations Containing Surfactants with Chitin or Chitosan Derivatives as Stabilizing Agents," Jpn. Kokai Tokkyo Koho JP 63,165,307 [88,165,307].
102.
Iwao, S.1989. "Hair Preparations Containing Polyoxyalkylene and Water-Soluble Chitin and Chitosan Derivatives for Protecting Hair," Jpn. Kokai Tokkyo Koho JP 01,09,911 [89,09,911].
103.
Bandai, H. , I. Watanabe and K. Murayama.1988. "Bath Preparations Containing Chitosan or Its Derivatives," Jpn. Kokai Tokkyo Koho JP 63,10,715 [88,10,715].
104.
Komiyama, N. and H. Itoi.1988. "Denture Cleaners Containing Water-Soluble Chitins and Chitosans," Jpn. Kokai Tokkyo Koho JP 63,267,361 [88,267,361].
105.
Kise, H., A. Hayakawa and H. Noritomi.1987. Biotech. Lett., 9(8): 543-548.
106.
Muzzarelli, R.A.A., A. Barontini and R. Rocchetti.1976. Biotech. Bioeng. , 18:1445-1454.
107.
Ohtakara, A. , M. Mitsutomi and Y. Uchida.1986. In Chitin in Nature and Technology, R. Muzzarelli, C. Jeuniaux and G. W. GooDay , eds., New York: Plenum Press , pp. 409-411.
108.
Illanes, A., R. Chamy and M.E. Zuniga.1986. In Chitin in Nature and Technology , R. Muzzarelli, C. Jeuniaux and G. W. GooDay, eds., New York: Plenum Press, pp. 411-415.
109.
Sicsic, S., J. Leonil, J. Braun and F. LeGoffic.1986. In Chitin in Nature and Technology , R. Muzzarelli, C. Jeuniaux and G. W. GooDay, eds., New York: Plenum Press, pp. 420-422.
110.
Synowiecki, J.1986. In Chitin in Nature and Technology , R. Muzzarelli, C. Jeuniaux and G. W. GooDay , eds., New York: Plenum Press , pp. 417-420.
111.
Van Griethuysen, E., E. Flaschel and A. Renken.1986. In Chitin in Nature and Technology , R. Muzzarelli, C. Jeuniaux and G. W. GooDay, eds., New York: Plenum Press, pp. 422-428.
112.
Nakakuki, T. , M. Yoshida and M. Okada.1988. "Immobilization of Cyclodextrin Glucanotransferase on Chitosan Beads," Jpn. Kokai Tokkyo Koho JP 63,196,290 [88,196,290].
113.
Braun, J., C.P. Le and G.F. Le.1989. Biotechnol. Bioeng., 33(2):242-246.
Champluvier, B., F. Marchal and P.G. Rouxhet.1989. Enzyme Microb. Technol. , 11(7):422-430.
117.
Yoshioka, T., R. Hirano, T. Shioya and M. Kako.1990. Biotech. Bioeng., 35:66-72.
118.
Sandford, P.A. and G.P. Hutchings.1987. In Industrial Polysaccharides, Progress in Biotechnology, M. Yalpani, ed., 3:363-376.
119.
Pospieszny, H. and I.G. Atabekov.1989. Plant Sci., 62(1):29-31.
120.
1989. United States Environmental Protection Agency, Poly-D-Glucosamine (Chitosan); Exemption from the Requirement of A Tolerance, Fed. Regist. 23 Mar. 1989, 54(55):11948-11949(Eng.).
Takahashi, N.1988. "Addition of Chitosan to Soils for Improvement of the Physical Properties," Jpn. Kokai Tokkyo Koho JP 63,146,982 [88,146,982].
123.
Nowosielski, O., H. Struszczyk, T. Skwarski, S. Kotlinski, K. Krajewski and W. Dziennik.1988. "Liquid Hydroponic Fertilizer Containing Chitosan and Trace Elements ," Pol. PL 141,381.
124.
Teixeira, M., W.P. Paterson, E.J. Dunn, Q. Li , B.K. Hunter and M.F.A. Goosen.1990. Ind. Eng. Chem. Res. , 29:1205-1209.
Imeri, A.G. and D. Knorr.1988. J. Food Sci. , 53(6):1707-1709.
127.
Soto-Peralta, N.V., H. Mueller and D. Knorr.1989. J. Food Sci., 54(2):495,496.
128.
Haga, M. and S. Enokida.1988. "Chitosan-Containing Preservatives and Sterilants for Foods," Jpn. Kokai Tokkyo Koho JP 63,169,975 [88,169,975].
129.
Asao, Y., K. Sakuma and T. Machama.1989. "Preservatives Containing Chitosan and Ascorbic Acid for Seafoods," Jpn. Kokai Tokkyo Koho JP 01,71,439 [89,71,439].
130.
Kin, M., K. Kikuchi, I. Yamamoto and N. Yagi.1988. "Milk Protein-Chitosan Complexes for Kamaboko and Other Products," Jpn. Kokai Tokkyo Koho JP 63,169,939 [88,169,939].
131.
Izume, M. and Y. Uchida.1988. "Food Preservation and Its Enhancement with Chitosan and/or Chitosanase-Digestion Products," Jpn. Kokai Tokkyo Koho JP 63,251,072 [88,251,072].
132.
Wakayama, Y. and Y. Sekino.1987. "Stabilization of Coloring Agents and Coloured Products with Chitosan," Jpn. Kokai Tokkyo Koho JP 62,297,365 [877,297,365].
133.
Kuwahara, Y., N. Otsuka and M. Manabe.1988. Nippon Shokuhin Kogyo Gakkaishi, 35(11):776-780.
134.
Kono, M., T. Matsui and C. Shimizu.1987. Nippon Suisan Gakkaishi, 53(1):125-129.
135.
Moore, G.K. and G.A.F. Roberts.1980. Int. J. Biol. Macromol. , 2:115.
136.
Roberts, G.A.F. and K.E. Taylor.1989. Makromol. Chem., 190(5): 951-960.
137.
Lim, F. and A.M. Sun.1980. Science, 210:908-910.
138.
Lim, F. and Richmond, VA.1982. "Encapsulation of Biological Materials," U.S. Patent 4,352,883.
139.
Rha, C. and D. Rodriguez-Sanchez .1988. "Encapsulated Active Material System;' U.S. Patent No. 4,749,620.
140.
Green, B.K.1955. "Pressure-Sensitive Record Materials;' U.S. Patent No. 2,712,507.
141.
Chang, T.M.S. , Science, 146:524,525.
142.
Sun, A.M. and G.M. O'Shea.1985. J. Control. Rel., 2:137-141.
143.
King, G.A. , A.J. Daugulis, P. Faulkner and M.F.A. Goosen.1987. Biotech. Progress, 3(4):231-240.
144.
Posillico, E.G.1986. Biotechnol., 4(2):114-117.
145.
Rupp, R.G.1985. In Large-Scale Mammalian Cell Culture, J. Feder and W. R. Tolbert , eds., Academic Press, Inc., pp. 19-38.
146.
King, G.A. , A.J. Daugulis, P. Faulkner, D. Bayly and M.F.A. Goosen.1989. Biotech. Bioeng., 34:1085-1091.
147.
Kim, S.K. and C. Rha.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P Sandford, eds., New York: Elsevier Applied Science, pp. 617-626.
148.
Goosen, M.F.A. , G.A. King, C.A. McKnight and N. Marcotte.1989. J. Membrane Science , 41:323-343.
149.
Goosen, M.F.A.1987. In CRC Critical Reviews in Biocompatibility, D. F. Williams, ed., Boca Raton, FL: CRC Press. p. 1.
150.
Daly, M.M. and D. Knorr.1988. Biotech. Progress, 4(2):76-81.
151.
Daly, M.M., R.W. Keown and D.W. Knorr.1989. "Chitosan Alginate Capsules," U.S. Patent No. 4,808,707.
152.
Shioya, T. and C. Rha.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp. 627-634.
153.
Kim, S.K. and C. Rha.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp. 627-642.
154.
Markey, M.L., L.M. Bowman and M.V.W. Bergamini .1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp. 713-717.
155.
Brine, C.J.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York : Elsevier Applied Science, pp. 679-691.
156.
Poole, S.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P Sandford, eds., New York : Elsevier Applied Science, pp. 523-531.
157.
Roberts, G.A.F. and K.E. Taylor.1989. In Chitin and Chitosan , G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science , pp. 577-583.
158.
Seo, H. and Y. Kinemura.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp.585-588.
159.
Holland, C.R.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York: Elsevier Applied Science, pp. 559-566.
160.
Nilsson, K. and K. Mosbach.1980. FEBS Lett. , 118(1):145-150.
161.
Uragami, T.1989. In Chitin and Chitosan, G. Skjak-Braek, T. Anthonsen and P. Sandford, eds., New York : Elsevier Applied Science, pp. 783-792.
162.
Sefton, M.V. and R.L. Broughton.1982. Biochimica et Biophys . Acta, 717:473-477.
163.
Lamberti, F.V., M.A. Wheatley, R.A. Evangelista and M.V. Sefton.1983. Am. Chem. Soc., Polym. Prepr., 24(1):75,76.
164.
Gharapetian, H., M. Maleki, N.A. Davies and A.M. Sun.1986. Polym. Mater. Sci. Eng. , 54:114-118.
165.
Tice, T.R. and W.E. Meyers.1985. Eur. Pat. Application, No. 0,129,619.
