The selective labeling of polysaccharides by the hydroxyl groups and without loosing biological activity is described. The new reagent is a deriv ative of fluoresceinamine and should be useful for the appropriate labeling of several types of biological active materials containing both amino and hydroxyl functional groups.
Get full access to this article
View all access options for this article.
References
1.
De Belder, A.N. and K. Granath.1973. Carbohyd. Res , 3:375.
2.
Casu.1985. Adv. Carbohyd. Chem. Biochem., 43:51.
3.
Glabe, C.G., P.K. Harty and S.D. Rosen.1983. Anal. Biochem., 130:287.
4.
Nagasawa, K. and H. Uchiyama.1978. Biochim. Biophys. Acta., 544:430.
5.
Jones, G.R., R. bt.Hashim and D.M. Power.1987. Biochim. Biophys. Acta., 925:57.
6.
Cardin, A.D. , K.R. Witt and R.L. Jackson.1984. Anal. Biochem., 137:368.
7.
Teien, A.N. and M. Lie.1977. Throb Res., 10:399.
8.
Blumenkrantz, N. and G. Asboe-Hansen.1973. Anal. Biochem. , 54:484.