A well-defined heparin fraction, H1080 82, which was obtained previously from a commercial heparin, has been extrasulfated by the triethylamine/SO 3 complex method in DMF. Two extrasulfated E1 and E20 samples were obtained after 1 h and 20 h reaction, respectively. Anticoagulant and anti-Xa activities, potentiometric titration, conductometric titration, 13 C NMR and CD spectra of the starting fraction and of the two extrasulfated samples are described. In spite of the rather important chemical modifications thus achieved, biological activities are retained to a great extent.
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