Fabrication of a biocompatible nanofibrous dressing with the advantage of the inclusion of bioactive herbal extracts is a promising approach in skin tissue engineering and wound healing applications. Herbal extracts possess many properties to promote the wound healing process, such as antioxidant properties, anti-inflammation activities as well as enhancing fibroblasts proliferation and migration.
In this study, Calendula officinalis (C. officinalis) and coffee extracts were loaded into poly(vinyl alcohol)/poly(ɛ-caprolactone) (PVA/PCL) nanofibrous mats. The obtained scaffolds were then characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), contact angle, and mechanical measurements. Also, the antioxidant activity, scratch assay, and cell viability of fibroblast cells were also evaluated. The results showed PVA/PCL scaffold loaded with 10 wt% C. officinalis and coffee extracts displayed smooth homogenous morphology with 317 ± 106 nm average diameter. Moreover, the relevant analyses confirmed that the extracts were incorporated into the nanofibers with suitable hydrophilicity and higher mechanical strength (4 ± 0.4 MPa). The antioxidant assay showed that IC50 values of coffee and C. officinalis extracts were 46 ± 1 ppm and 101 ± 4 ppm, successively, which presented a high antioxidant activity. The combination of both extracts showed a higher rate of migration than individual extracts with not detected cytotoxic effects on the human dermal fibroblast cells. In conclusion, our results confirmed that the coffee and C. officinalis extracts loaded PVA/PCL nanofibrous scaffolds could provide an appropriate construct for wound healing applications.
BoerMDuchnikEMaleszkaR, et al. Structural and biophysical characteristics of human skin in maintaining proper epidermal barrier function. Adv Dermatology Allergol2016; 1: 1–5.
2.
El-AassarMRIbrahimOMFoudaMMG, et al. Wound healing of nanofiber comprising polygalacturonic/hyaluronic acid embedded silver nanoparticles: in-vitro and in-vivo studies. Carbohydr Polym2020; 238: 116175.
3.
EmingSAMartinPTomic-CanicM.Wound repair and regeneration: mechanisms, signaling, and translation. Sci Transl Med2014; 6: 265sr6.
4.
ShedoevaALeavesleyDUptonZ, et al. Wound healing and the use of medicinal plants. Evid Based Complement Alternat Med2019; 2019: 1–30.
5.
MiguelSPSequeiraRSMoreiraAF, et al. An overview of electrospun membranes loaded with bioactive molecules for improving the wound healing process. Eur J Pharm Biopharm2019; 139: 1–22.
6.
NaeimiAPayandehMGharaAR, et al. In vivo evaluation of the wound healing properties of bio-nanofiber chitosan/polyvinyl alcohol incorporating honey and Nepeta dschuparensis. Carbohydr Polym2020; 240: 116315.
7.
WellerCWellerCTeamV.Interactive dressings and their role in moist wound management. In: WellerCTeamV (eds) Advanced textiles for wound care. Sawston: Woodhead Publishing, 2019, pp.105–134.
8.
BenskinLL.Commentary: first-line interactive wound dressing update: a comprehensive review of the evidence. Front Pharmacol2020; 11: 155.
9.
de VriesK. Primary care: hydrocolloid and hydroactive wound dressings. Aust J Pharm2018; 99: 81–83.
10.
LiangYHeJGuoB.Functional hydrogels as wound dressing to enhance wound healing. ACS Nano2021; 15: 12687–12722.
11.
KoehlerJBrandlFPGoepferichAM.Hydrogel wound dressings for bioactive treatment of acute and chronic wounds. Eur Polym J2018; 100: 1–11.
12.
LiuXXuHZhangM, et al. Electrospun medicated nanofibers for wound healing: review. Membranes2021; 11: 770.
13.
AzimiBMalekiHZavagnaL, et al. Bio-based electrospun fibers for wound healing. J Funct Biomater2020; 11: 67.
14.
Pilehvar-SoltanahmadiYDadashpourMMohajeriA, et al. An overview on application of natural substances incorporated with electrospun nanofibrous scaffolds to development of innovative wound dressings. Mini Rev Med Chem2018; 18: 414–427.
15.
LiuMDuanX-PLiY-M, et al. Electrospun nanofibers for wound healing. Mater Sci Eng C Mater Biol Appl2017; 76: 1413–1423.
16.
BraghirolliDISteffensDPrankeP.Electrospinning for regenerative medicine: a review of the main topics. Drug Discov Today2014; 19: 743–53.
17.
MalekiHKhoshnevisanKSajjadi-JaziSM, et al. Nanofiber-based systems intended for diabetes. J Nanobiotechnology2021; 19: 317.
18.
DoostanMMalekiHDoostanM, et al. Effective antibacterial electrospun cellulose acetate nanofibrous patches containing chitosan/erythromycin nanoparticles. Int J Biol Macromol2021; 168: 464–473.
19.
ChenSLiuBCarlsonMA, et al. Recent advances in electrospun nanofibers for wound healing. Nanomedicine2017; 12: 1335–1352.
20.
MemicAAbudulaTMohammedHS, et al. Latest progress in electrospun nanofibers for wound healing applications. ACS Appl Bio Mater2019; 2: 952–969.
21.
BuiHTChungOHDela CruzJ, et al. Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing. Macromol Res2014; 22: 1288–1296.
22.
Ranjbar-MohammadiMArab-BafraniZKarimiF, et al. Designing hybrid nanofibers based on keratin-poly (vinyl alcohol) and poly (ε-caprolactone) for application as wound dressing. J Ind Text2022; 51: 1729S–1949S.
23.
LiuYZhouSGaoY, et al. Electrospun nanofibers as a wound dressing for treating diabetic foot ulcer. Asian J Pharm Sci2019; 14: 130–143.
24.
Pedram RadZMokhtariJAbbasiM. Preparation and characterization of Calendula officinalis-loaded PCL/gum arabic nanocomposite scaffolds for wound healing applications. Iran Polym J2019; 28: 51–63.
25.
VinodMSinghMPradhanM, et al. Phytochemical constituents and pharmacological activities of betula alba linn. A review. Int J PharmTech Res2012; 4: 643–647.
HosseinkazemiHBiazarEBonakdarS, et al. Modification of PCL electrospun nanofibrous mat with calendula officinalis extract for improved interaction with cells. Int J Polym Mater Polym Biomater2015; 64: 459–464.
28.
Pedram RadZMokhtariJAbbasiM. Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. Int J Biol Macromol2019; 135: 530–543.
29.
GivolOKornhaberRVisentinD, et al. A systematic review of Calendula officinalis extract for wound healing. Wound Repair Regen2019; 27: 548–561.
30.
DindaMDasguptaUSinghN, et al. PI3K-mediated proliferation of fibroblasts by Calendula officinalis tincture: implication in wound healing. Phytother Res2015; 29: 607–616.
31.
AshwlayanVDKumarAVermaM, et al. Therapeutic potential of Calendula officinalis. Pharm Pharmacol Int J2018; 6: 149–155.
32.
VermaPKRainaRAgarwalS, et al. Phytochemical ingredients and pharmacological potential of Calendula officinalis Linn. Pharm Biomed Res2018; 4: 1.
33.
Humaryanto and Ave OR. Exploring the potential of green coffee extract for wound healing treatment. IOP Conf Ser Earth Environ Sci2019; 391: 012057.
34.
KenisaYPIstiatiIJWS. Effect of Robusta coffee beans ointment on full thickness wound healing. Dent J2012; 45: 52.
35.
AffonsoRCLVoytenaAPLFananS, et al. Phytochemical composition, antioxidant activity, and the effect of the aqueous extract of coffee (Coffea arabica L.) bean residual press cake on the skin wound healing. Oxid Med Cell Longev2016; 2016: 1–10.
36.
MoreiraDPMonteiroMCRibeiro-AlvesM, et al. Contribution of chlorogenic acids to the iron-reducing activity of coffee beverages. J Agric Food Chem2005; 53: 1399–1402.
37.
dos SantosMDAlmeidaMCLopesNP, et al. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull2006; 29: 2236–2240.
38.
HoelzlCKnasmüllerSWagnerK-H, et al. Instant coffee with high chlorogenic acid levels protects humans against oxidative damage of macromolecules. Mol Nutr Food Res2010; 54: 1722–1733.
39.
DeepthiSNivedhitha SundaramMDeepti KadavanJ, et al. Layered chitosan-collagen hydrogel/aligned PLLA nanofiber construct for flexor tendon regeneration. Carbohydr Polym2016; 153: 492–500.
40.
Pedram RadZMokhtariJAbbasiM. Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. Int J Biol Macromol2019; 135: 530–543.
41.
BelchiorVBotelhoBGCasalS, et al. FTIR and chemometrics as effective tools in predicting the quality of specialty coffees. Food Anal Methods2020; 13: 275–283.
42.
BarceloJMGatchallanAMAquinoIJB, et al. FTIR spectrum and antimutagenicity of coffea arabica pulp and arachis hypogaea testa in relation to their in vitro antioxidant properties. Asia Pacific J Multidiscip Res2015; 3: 99–108.
43.
AliSKhatriZOhKW, et al. Preparation and characterization of hybrid polycaprolactone/cellulose ultrafine fibers via electrospinning. Macromol Res2014; 22: 562–568.
44.
PrabhaRDKraftDCEHarknessL, et al. Bioactive nano-fibrous scaffold for vascularized craniofacial bone regeneration. J Tissue Eng Regen Med2018; 12: e1537–e1548.
45.
Gómez-RuizJALeakeDSAmesJM.In vitro antioxidant activity of coffee compounds and their metabolites. J Agric Food Chem2007; 55: 6962–6969.
46.
LiangNKittsD.Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules2014; 19: 19180–19208.
47.
PreethiKCKuttanGKuttanR.Antioxidant potential of an extract of Calendula officinalis flowers in vitro and in vivo. Pharm Biol2006; 44: 691–697.
48.
GradaAOtero-VinasMPrieto-CastrilloF, et al. Research techniques made simple: analysis of collective cell migration using the wound healing assay. J Invest Dermatol2017; 137: e11–e16.
49.
NicolausCJunghannsSHartmannA, et al. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. J Ethnopharmacol2017; 196: 94–103.
50.
FronzaMHeinzmannBHamburgerM, et al. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol2009; 126: 463–467.
