Development of a process for the manufacturing of SU-8 100 for the use in cell culture

Abstract

SU-8 100 is a negative-tone photoresist which is used for the manufacturing of structures with high thickness and aspect ratio. The use of SU-8 100 systems in cell culture is difficult due to its cytotoxicity resulting from leachables. In this article, the production process of non-cytotoxic SU-8 100 foils is described. The aim is to create a polymer scaffold with integrated sensors in order to enable online measurements of cell growth and viability within a three-dimensional cell culture. This scaffold could be a useful tool for testing biological effects of pharmaceutical substances. Therefore, the scaffold material must not have a negative influence on the cell growth and viability. Among the biocompatibility tests described in DIN EN ISO 10993-5, the neutral red uptake method was chosen to evaluate the cytotoxicity of polymer foils. The production process of SU-8 100 was altered in such a way that non-cytotoxic SU-8 100 foils can be manufactured.

Keywords

Cytotoxicity leachables neutral red uptake SU-8 100 infrared spectroscopy

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References

Qin

Yeh

Hao

et al . Developing an ultra non-fouling SU-8 and PDMS hybrid microfluidic device by poly(amidoamine) engraftment. Colloids Surf B Biointerfaces 2015; 127: 247–255.

Kotzar

Freas

Abel

et al . Evaluation of MEMS materials of construction for implantable medical devices. Biomaterials 2002; 23(13): 2737–2750.

Hennemeyer

Walther

Kerstan

et al . Cell proliferation assays on plasma activated SU-8. Microelectron Eng 2008; 85(5–6): 1298–1301.

Glawdel

Ren

et al . Fabrication of a hybrid PDMS/SU-8/quartz microfluidic chip for enhancing UV absorption whole-channel imaging detection sensitivity and application for isoelectric focusing of proteins. Lab Chip 2009; 9(13): 1926–1932.

Nemani

Moodie

Brennick

et al . In vitro and in vivo evaluation of SU-8 biocompatibility. Mat Sci Eng C: Mater 2013; 33(7): 4453–4459.

Cho

Cauller

et al . Biocompatible SU-8-based microprobes for recording neural spike signals from regenerated peripheral nerve fibers. IEEE Sens J 2008; 8(11): 1830–1836.

Stangegaard

Wang

Kutter

et al . Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces. Mol BioSyst 2006; 2(9): 421–428.

Vernekar

Cullen

Fogleman

et al . SU-8 2000 rendered cytocompatible for neuronal bioMEMS applications. J Biomed Mater Res A 2009; 89(1): 138–151.

Olziersky

Barquinha

Vilà

et al . Insight on the SU-8 resist as passivation layer for transparent Ga₂O₃–In₂O₃–ZnO thin-film transistors. J Appl Phys 2010; 108(6): 64505-1–64505-7.

10.

Mitri

Birarda

Vaccari

et al . SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells. Lab Chip 2014; 14(1): 210–218.

11.

Sears

Soucek

et al . Synthesis of reactive diluents for cationic cycloaliphatic epoxide UV coatings. Polymer 1999; 40(20): 5675–5686.

12.

Keller

Blagoi

Lillemose

et al . Processing of thin SU-8 films. J Micromech Microeng 2008; 18(12): 125020.

13.

Abadie

MJM

Chia

Boey

Cure kinetics for the ultraviolet cationic polymerization of cycloaliphatic and diglycidyl ether of bisphenol-A (DGEBA) epoxy systems with sulfonium salt using an auto catalytic model. J Appl Polym Sci 2002; 86(7): 1587–1591.

14.

Versace

D-L

Dubot

Cenedese

et al . Natural biopolymer surface of poly(3-hydroxybutyrate-co-3-hydroxy-valerate)-photoinduced modification with triarylsulfonium salts. Green Chem 2012; 14(3): 788–798.

15.

Agarwal

Gunasekaran

Coane

et al . Scum-free patterning of SU-8 resist for electroforming applications. J Micromech Microeng 2004; 15(1): 130–135.

16.

Gebel

Arsenic and antimony: comparative approach on mechanistic toxicology. Chem-Biol Interact 1997; 107(3): 131–144.

17.

Léonard

Gerber

GB.

Mutagenicity, carcinogenicity and teratogenicity of antimony compounds. Mutat Res/Rev Genet 1996; 366(1): 1–8.

18.

Tsutsui

Suzuki

Ohmori

et al . Cytotoxicity, chromosome aberrations and unscheduled DNA synthesis in cultured human diploid fibroblasts induced by sodium fluoride. Mutat Res Lett 1984; 139(4): 193–198.

19.

Melai

Salm

Wolters

et al . Qualitative and quantitative characterization of outgassing from SU-8. Microelectron Eng 2009; 86(4–6): 761–764.