Biocompatible and antimicrobial wound dressings are important biomaterials for tissue adhesion and healing. Hydrogels derived from natural polyamino acids are ideal wound dressing because of their good water solubility, biocompatibility, and biodegradability. In this study, we introduce poly(amino acid)–based hydrogels as a new type of wound dressing for wound healing. The composite hydrogels were derived from water-soluble ε-polylysine and γ-poly(glutamic acid) cross-linked by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxy-succinimide. The gelation is attributed to the reaction between the amino groups of ε-polylysine and carboxyl groups of γ-poly(glutamic acid). Fourier transform infrared spectroscopy was used to characterize the composite hydrogels. The effect of the ratio of ε-polylysine and γ-poly(glutamic acid) on the gelation time, surface morphology, equilibrium swelling, elastic moduli, adhesive strength, and in vitro degradation of composite hydrogels was examined. The hydrogels showed excellent antimicrobial efficacy against Escherichia coli and Staphylococcus aureus. Indirect cytotoxicity assessment indicated that the composite hydrogels were nontoxic to the L929 cell. The potential of the composite hydrogel as wound dressing was demonstrated by applying it in the skin incision. Results demonstrated that the composite hydrogel showed superior healing effects compared with suture, fibrin glue, and compont skin adhesive, while eliciting less inflammatory response. In conclusion, antimicrobial and biocompatible ε-polylysine–γ-poly(glutamic acid) composite may have great application for wound healing.
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