This study aims to extract keratin from quail feather wastes and incorporate it with silver nanoparticles into a synthetic biodegradable polymer in order to fabricate a nanofibrous scaffold with improved biomedical properties. Polyvinyl alcohol was used as the host polymer and spinning dopes with different amounts (0, 0.15, and 0.75 wt %) of extracted keratin and the same amount of silver nanoparticles prepared in order to fabricate scaffolds. According to the results, the scaffolds with a higher amount of extracted keratin (i.e. 0.75 wt %) provided less bead formation and more uniformity; also, they gave 99.9% and 98% of the antibacterial activity against gram negative (Escherichia coli) and gram positive (Staphylococcus aureus) bacteria, respectively. The analysis of the biological response of fibroblast cells cultured on the synthetic scaffolds exhibited remarkable improvement in comparison to the pristine (polyvinyl alcohol-Ag) scaffolds. This article concludes that the addition of extracted keratin into a polymeric matrix (polyvinyl alcohol) can improve both antibacterial properties and cell viability for the resultant scaffolds, and this qualifies them as potent candidates for biomedical applications.
VaresanoAAluigiAVineisC. Study on the shear viscosity behavior of keratin/PEO blends for nanofibre electrospinning. J Polym Sci B Polym Phys2008; 46(12): 1193–1201.
2.
LiXMFengQLJiaoYF. Collagen-based scaffolds reinforced by chitosan fibres for bone tissue engineering. Polym Int2005; 54: 1034–1040.
3.
RouseIGVan DykeME.A review of keratin-based biomaterials for biomedical applications. Materials2010; 3(2): 999–1014.
4.
MalafayaPBSilvaGAReisRL.Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv Drug Deliv Rev2007; 59(4–5): 207–233.
5.
SellSAWolfePSGargK. The use of natural polymers in tissue engineering: a focus on electrospun extracellular matrix analogues. Polymers2010; 2(4): 522–553.
6.
BoatengJSMatthewsKHStevensHN. Wound healing dressings and drug delivery systems: a review. J Pharm Sci2008; 97(8): 2892–2923.
DhandayuthapaniBYoshidaYMaekawaT.Polymeric scaffolds in tissue engineering application: a review. Int J Polym Sci2011; 2011: 1–19.
9.
CilurzoFSelmiFAluigiA. Regenerated keratin proteins as potential biomaterial for drug delivery. Polym Adv Technol2013; 24(11): 1025–1028.
10.
SrinivasanBKumarRShanmugamK. Porous keratin scaffold-promising biomaterial for tissue engineering and drug delivery. J Biomed Mater Res B Appl Biomater2010; 92(1): 5–12.
11.
LiXWangLFanY. Nanostructured scaffolds for bone tissue engineering. J Biomed Mater Res A2013; 101(8): 2424–2435.
12.
CantuDAKaoWJ.Combinatorial biomatrix/cell-based therapies for restoration of host tissue architecture and function. Adv Healthc Mater2013; 2(12): 1544–1563.
13.
GohYFShakirIHussainR.Electrospun fibers for tissue engineering, drug delivery, and wound dressing. J Mater Sci2013; 48(8): 3027–3054.
14.
LiXYangYFanY. Biocomposites reinforced by fibers or tubes as scaffolds for tissue engineering or regenerative medicine. J Biomed Mater Res A2014; 102(5): 1580–1594.
15.
GazzarriMBartoliCPuppiD. Fibrous star poly(ε-caprolactone) melt-electrospun scaffolds for wound healing applications. J Bioact Compat Polym2013; 28(5): 492–507.
16.
TamC.In vivo efficacy of Keratin-Derived Antimicrobial Peptides (KDAMPs) in corneal defense against pseudomonas aeruginosa. Inves Ophtalmol Vis Sci2013; 54(9): 6052–6062.
17.
HajmalekiMKhajaviRToliyatT.Cytotoxicity and antibacterial properties of nano fibrous scaffolds based on extracted keratin from human hair waste and silver nano particles. Med Sci J Islamic Azad Univ2015; 25(1): 46–54.
18.
LeeHYeoMAhnS. Designed hybrid scaffolds consisting of polycaprolactone microstrands and electrospun collagen nanofibers for bone tissue engineering. J Biomed Mater Res B Appl Biomater2011; 97(2): 263–270.
19.
LienSMKoLYHuangTJ.Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering. Acta Biomater2009; 5(2): 670–679.
20.
TachibanaAKanekoSTanabeT. Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation. Biomaterials2005; 26(3): 297–302.
21.
ReichlS.Films based on human hair keratin as substrates for cell culture and tissue engineering. Biomaterials2009; 30(36): 6854–6866.
22.
AboushwarebTEberliDWardC. A Keratin biomaterial gel hemostat derived from human hair: evaluation in a rabbit model of lethal liver injury. J Biomed Mater Res B Appl Biomater2009; 90(1): 45–54.
23.
JagadeeshgoudaKBReddyPRIshwaraprasadK.Experimental study of behavior of poultry feather fiber- a reinforcing material for composites. Int J Res Eng Technol2014; 3(2): 362–371.
24.
ReddyNYangY.Structure and properties of chicken feather barbs as natural protein fibers. J Polym Environ2007; 15: 81–87.
25.
KhosaMAUllahA.A sustainable role of keratin biopolymer in green chemistry: a review. J Food Process Beverage2013; 1(1): 1–8.
26.
SenJ.Human hair in personal identification and documenting drug and substance abuse. Anthropologist2010; 12(1): 47–58.
27.
SierpinskiPGarrettJMaJ. The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials2008; 29(1): 118–127.
28.
SowWTLuiSNgKW.Electrospun human keratin matrices as templates for tissue regeneration. Nanomedicine2013; 8(4): 531–541.
29.
LiJLiYLiY. Preparation and biodegradation of electrospun PLLA/keratin nonwoven fibrous membrane. Polym Degrade Stab2009; 94(10): 1800–1807.
30.
XuHCaiSXuL. Water-stable three-dimension ultrafine scaffolds from keratin for cartilage tissue engineering. Langmuir2014; 30(28): 8461–8470.
31.
BurnettLRRahmanyMBRichterJR. Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. Biomaterials2013; 34(11): 2632–2640.
32.
RadZPTavanaiHMoradiAR.Production of feather keratin nanopowder through electrospraying. J Aerosol Sci2012; 51: 49–56.
33.
WangYXLaoXJ.Extracting keratin from chicken feather by using a hydrophobic ionic liquid. Process Biochem2012; 47(5): 896–899.
ShihJCH. Recent developments in poultry waste digestion and feather utilization: a review. Poultry Sci1993; 72(2–3): 1617–1620.
36.
SunPLiuZTLiuZW.Particles from bird feather: a novel application of an ionic liquid and waste resource. J Hazard Mater2009; 170: 786–790.
37.
XuHShiZReddyN. Intrinsically water-stable keratin nanoparticles and their in vivo biodistribution for targeted delivery. J Agric Food Chem2014; 62(37): 9145–9150.
38.
IdrisAVijayaraghavanRPattiAF. Distillable protic ionic liquids for keratin dissolution and recovery. ACS Sustainable Chem Eng2014; 2(7): 1888–1894.
39.
XuHYangY.Controlled de-cross-linking and disentanglement of feather keratin for fiber preparation via a novel process. ACS Sustainable Chem Eng2014; 2(6): 1404–1410.
40.
SaravananSSameeraDKMoorthiA. Chitosan scaffolds containing chicken feather keratin nanoparticles for bone tissue engineering. Int J Biol Macromol2013; 62: 481–486.
41.
YinXCLiFYHeYF. Study on effective extraction of chicken feather keratins and their films for controlling drug release. Biomater Sci2013; 1(5): 528–536.
42.
GuoJPanSYinX. pH-sensitive keratin-based polymer hydrogel and its controllable drug-release behavior. J Appl Polym Sci2015; 132(9): 41572 (1–8).
43.
ReddyNJiangQJinE. Bio-thermoplastics from grafted chicken feathers for potential biomedical applications. Colloids Surf B Biointerfaces2013; 110: 51–58.
44.
GaidauCPeticaACiobanuC. Investigations on antimicrobial activity of collagen and keratin based materials doped with silver nanoparticles. Rom Biotechnol Lett2009; 14(5): 4665–4672.
KatohKShibayamaMTanabeT. Preparation and properties of keratin–poly(vinyl alcohol) blend fiber. J Appl Polym Sci2004; 91(2): 756–762.
47.
LiSYangXH.Fabrication and characterization of electrospun wool keratin/poly(vinyl alcohol) blend nanofibers. Adv Mater Sci Eng2014; 2014: 163678 (1–7 pp.).
48.
MarsichELBellomoFTurcoG. Nano-composite scaffolds for bone tissue engineering containing silver nanoparticles: preparation, characterization and biological properties. J Mater Sci Mater Med2013; 24(7): 1799–1807.
49.
El-KadyAMRizkRAEl-HadyBMA. Characterization, and antibacterial properties of novel silver releasing nanocomposite scaffolds fabricated by the gas foaming/salt-leaching technique. J Genet Eng Biotechnol2012; 10(2): 229–238.
50.
BaruaSChattopadhyayPAidewL. Infection-resistant hyperbranched epoxy nanocomposite as a scaffold for skin tissue regeneration. Polym Int2015; 64(2): 303–311.
51.
SchneiderODLoherSBrunnerTJ. Flexible, silver containing nanocomposites for the repair of bone defects: antimicrobial effect against E. coli infection and comparison to tetracycline containing scaffolds. J Mater Chem2008; 18(23): 2679–2684.
52.
GuptaANuruldiyanahBKKeeCYG. Extraction of keratin protein from chicken feather. J Chem Chem Eng2012; 6(8): 732–741.
53.
LaemmliUK.Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970; 227(5259): 680–685.
54.
KhajaviRAbbasipourM.Electrospinning as a versatile method for fabricating coreshell, hollow and porous nanofibers. Sci Iran2012; 19(6): 2029–2034.
55.
AbbasipourMKhajaviR.Nanofiber bundles and yarns production by electrospinning: a review. Adv Polym Tech2013; 32(3): 1–12.
56.
KhajaviRJahangirian EsfahaniESattariM.Crystalline structure of microbial cellulose compared with native and regenerated cellulose. Int J Polym Mater2011; 60(14): 1178–1192.
57.
AbbasipourMMirjaliliMKhajaviR. Coated cotton gauze with Ag/ZnO/chitosan nanocomposite as a modern wound dressing. J Eng Fabr Fiber2014; 9(1): 124–130.
58.
AATCC Test Method 100-2004. Assessment of antibacterial finishes on textile materials: AATCC technical manual. Research Triangle Park, NC: American Association of Textile Chemists and Colorists (AATCC), 2005.
59.
BonakdarSHEmamiSHShokrgozarMA. Preparation and characterization of polyvinyl alcohol hydrogels crosslinked by biodegradable polyurethane for tissue engineering of cartilage. Mater Sci Eng C2010; 30(4): 636–642.
60.
HomaeigoharSSHShokrgozarMASadiAY. In vitro evaluation of biocompatibility of beta-tricalcium phosphate-reinforced high-density polyethylene; an orthopedic composite. J Biomed Mater Res A2005; 75(1): 14–22.
61.
AttiaGAbd El-KaderMFH. Structural, optical and thermal characterization of PVA/2HEC polyblend films. Int J Electrochem Sci2013; 8: 5672–5687.
62.
RautARMurhekarGH.Modification of cationic polymer conjugates using advance chemical methods. Int J Multidiscip Res Dev2014; 1(4): 9–13.
63.
FriedlanderHNHarrisHEPritchardJG. Structure–property relationships of poly(vinyl alcohol). I. Influence of polymerization solvents and temperature on the structure and properties of poly(vinyl alcohol) derived from poly(vinyl acetate). J Polym Sci A Polym Chem2003; 4(3): 649–664.
64.
ZhangH.Electrospun poly (lactic-co-glycolic acid)/multiwalled carbon nanotubes composite scaffolds for guided bone tissue regeneration. J Bioact Compat Polym2011; 26(4): 347–362.
65.
AluigiAZoccolaMVineisC. Study on the structure and properties of wool keratin regenerated from formic acid. Int J Biol Macromol2007; 41(3): 266–273.
66.
DickersonMBSierraAABedfordNM. Keratin-based antimicrobial textiles, films, and nanofibers. J Mater Chem B2013; 1(40): 5505–5514.
67.
TanabeTOkitsuNTachibanaA. Preparation and characterization of keratin-chitosan composite film. Biomaterials2002; 23(3): 817–825.
68.
LiLLiYLiJ. Cytotoxicity and cell adhesion of PLLA/keratin composite fibrous membranes. IFBME Proc2009; 23: 1492–1995.
