Sustained release of antibiotics from uniform poly (ε-caprolactone) microspheres prepared by a simple fluidic device with a tapered glass capillary

Abstract

Uniform poly(ε-caprolactone) microspheres containing a variety of water-soluble antibiotics, such as tobramycin, vancomycin, and gentamicin, were prepared by a simple fluidic device with a pristine or tapered glass capillary. Each type of antibiotic was dispersed in an organic solvent by ball-milling prior to microsphere preparation. The poly(ε-caprolactone) organic solution containing the powder of each antibiotic was introduced as the discontinuous phase into the fluidic device, where an aqueous phase containing surfactant served as the continuous phase. The poly(ε-caprolactone) microspheres were obtained after solvent evaporation. A tapered glass capillary was tested to produce poly(ε-caprolactone) microspheres, leading to the size reduction of the microspheres from 47.46 ± 0.72 to 25.49 ± 1.05 µm without destroying size uniformity. This size range should be suitable for parenteral injection into the human body. The release analysis revealed that gentamicin and vancomycin were released from the poly(ε-caprolactone) microspheres up to approximately 2 months in a more sustained manner than tobramycin, which is due to the solubility difference in the antibiotics in water. The antimicrobial activities of each type of antibiotic released from the poly(ε-caprolactone) microspheres were evaluated using Staphylococcus aureus and Escherichia coli.