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Abstract

In this study, the in vivo soft tissue response of chitosan/γ-poly(glutamic acid) (γ-PGA) polyelectrolyte complex (PEC) using a rat model was assessed using chitosan as a cationic polyelectrolyte and γ-PGA as an anionic polyelectrolyte; four groups of chitosan/γ-PGA PECs were synthesized according to the molar ratio of amine groups of chitosan to the carboxylic acid groups of γ-PGA. Different soft tissue responses to chitosan/γ-PGA PEC were observed between the epithelium and the muscle. In the epithelium, the wound dressed with chitosan/γ-PGA PEC healed faster than the control, with chitosan attracting polymorphonuclear (PMN) cells and γ-PGA creating a hydrophilic environment. In the muscle, a quantitative evaluation of the tissue response revealed that different degradation phenomena were evoked by different compositions of chitosan/γ-PGA PEC. After implantation for 28 days, the chitosan/γ-PGA PEC with chitosan dominating showed extensive surface erosion and superficial fragmentation surrounded by inflammatory cells, while chitosan/γ-PGA PECs with γ-PGA dominating elicited minimal degradation. These results confirm that the degradation of PECs can be controlled by tailoring the chitosan/γ-PGA PECs for different purposes. It appears that different local tissue conditions in muscle and epithelium may be involved in this difference.

Keywords

chitosan degradation γ-PGA polyelectrolyte complex polymorphonuclear cells tissue response

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Hou TY , Xu JZ , Li Q. , Feng JH and Zen L. In vitro evaluation of a fibrin gel antibiotic delivery system containing mesenchymal stem cells and vancomycin alginate beads for treating bone infections and facilitating bone formation. Tissue Eng Part A 2008; 14(7): 1173-1182.

Ip WY and Gogolewski S. Clinical application of resorbablen polymers in guided bone regeneration . Macromol Symp 2008; 253(1): 139-146.

Yoshimura T. , Matsuo K. and Fujioka R. Novel biodegradable superabsorbent hydrogels derived from cotton cellulose and succinic anhydride: synthesis and characterization. J Appl Polym Sci 2006; 99(6): 3251-3256.

Yang Z. , Zhang Y. , Markland P. and Yang VC Poly(glutamic acid) poly(ethylene glycol) hydrogels prepared by photoinduced polymerization: synthesis, characterization, and preliminary release studies of protein drugs. J Biomed Mater Res 2002; 62(1): 14-21.

Fang L. , Zhao Y. and Tan TW Preparation and water absorbent behavior of superabsorbent polyaspartic acid resin. J Polym Res 2006; 13(2): 145-152.

Buhus G. , Popa M. and Desbrieres J. Hydrogels based on carboxymethylcellulose and gelatin for inclusion and release of chloramphenicol. J Bioact Compat Polym 2009; 24(6): 525-545.

Mahkam M. Modified chitosan cross-linked starch polymers for oral insulin delivery . J Bioact Compat Polym 2010; 25(4): 406-419.

Tsai SP , Hsieh CY , Wang DM , Huang LLH , Lai JY and Hsieh HJ Preparation and cell compatibility evaluation of chitosan/collagen composite scaffolds using amino acids as crosslinking bridges. J Appl Polym Sci 2007; 105(4): 1774-1785.

Tsao CT , Chang CH , Lin YY , et al. Antibacterial activity and biocompatibility of a chitosan/ γ-poly(glutamic acid) polyelectrolyte complex hydrogel. Carbohydr Res 345(12): 1774-1780.

10.

Tsao CT , Chang CH , Lin YY , et al. Evaluation of chitosan/γ-poly(glutamic acid) polyelectrolyte complex for wound dressing materials. Carbohydr Polym 2010; doi:10.1016/j.carbpol.2010.04.034.

11.

Wu Y. , Chen N. , Liu LK , Yuan H. , Li QL and Chen SH Chitosan/alginate multilayer scaffold encapsulating bone marrow stromal cells in situ on titanium. J Bioact Compat Polym 2009; 24(4): 301-315.

12.

Bhattarai N. and Zhang MQ Controlled synthesis and structural stability of alginate-based nanofibers. Nanotechnology 2007; 18(45): 1-10.

13.

Wei XH , Niu YP , Xu YY , Du YZ , Hu FQ and Yuan H. Salicylic acid-grafted chitosan oligosaccharide nanoparticle for paclitaxel delivery. J Bioact Compat Polym 2010; 25(3): 319-335.

14.

Lin YH , Mi FL , Chen CT , et al. Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery. Biomacromolecules 2007; 8(1): 146-152.

15.

Lin YH , Chung CK , Chen CT , Liang HF , Chen SC and Sung HW Preparation of nanoparticles composed of chitosan/polyγ-glutamic acid and evaluation of their permeability through Caco-2 cells. Biomacromolecules 2005; 6(2): 1104-1112.

16.

Jansen JA , Dhert WJ , van der Waerden JP and von Recum AF Semi-quantitative and qualitative histologic analysis method for the evaluation of implant biocompatibility. J Invest Surg 1994; 7(2): 123-134.

17.

Shin H. , Ruhe PQ , Mikos AG and Jansen JA In vivo bone and soft tissue response to injectable, biodegradable oligo(poly(ethylene glycol) humarate) hydrogels. Biomaterials 2003; 24(19): 3201-3211.

18.

Ueno H. , Yamada H. , Tanaka I. , et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterial 1999; 20(15): 1407-1414.

19.

Muzzarelli RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr Polym 2009; 76(2): 167-182.

20.

Wang L. , Khor E. , Wee A and Lim LY. Chitosanalginate PEC membrane as a wound dressing: assessment of incisional wound healing. J Biomed Mater Res Part A 2002; 63(5): 610-618.

21.

Chiu CT , Lee JS , Chu CS , Chang YP and Wang YJ Development of two alginate-based wound dressings. J Mater Sci Mater Med 2008; 19(6): 2503-2513.

22.

Zheng Z. , Wei Y. , Wang G. , et al. Surface properties of chitosan films modified with polycations and their effects on the behavior of PC12 cells. J Bioact Compat Polym 2009; 24(1): 63-82.

23.

Valentini RF , Vargo TG , Gardella Jr JA and Aebischer P. Electrically charged polymeric substrates enhance nerve fibre outgrowth in vitro. Biomaterials 1992; 13(3): 183-190.

24.

Zhang Z. , Rouabhia M. , Wang Z. , et al. Electrically conductive biodegradable polymer composite for nerve regeneration: electricity-stimulated neurite outgrowth and axon regeneration . Artif Organs 2007; 31(1): 13-22.

Tissue response to chitosan/γ-PGA polyelectrolyte complex using a rat model

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