In this in vitro study, the initial response of human periodontal ligament (PDL) cells and alveolar osteoblast-like cells (HOB) to three biodegradable polymers with varying pore size and different mechanical properties were evaluated. Scaffolds were synthesized from poly(L-lactide), [poly(LLA)], poly(L-lactide-co-1,5-dioxepan-2-one), [poly(LLA-co-DXO)], poly(L-lactide-co-ε-caprolactone), and [poly(LLA-co-CL)] with pore sizes greater or less than 90 µm by salt leaching. Cells were obtained from patients undergoing routine oral surgery. After 2—4 passages, the cells were grown on scaffolds and in culture plates (control) for 3 h (PDL cells), 3 days (PDL cells and HOB), 10 and 14 days (HOB), respectively. The cellular morphology and spreading were determined by scanning electron microscopy (SEM) and the attachment and proliferation were evaluated by MTT assays. The SEM images revealed heterogeneous cellular morphology and good spreading. Cellular attachment and proliferation were significantly higher on poly(LLA-co-DXO) and poly(LLA-co-CL) than on poly(LLA) scaffolds (p = 0.003) and highest for poly(LLA-co-DXO). Expression of bone formation markers, collagen-I (COL-I), transforming growth factor-β 1 (TGF-β1), and osteocalcin (OCN), was determined by ELISA. The expression of COL-1 was similar for HOB and PDL cells, but significantly higher for pore size >90 µm while the HOB expression of TGFβ 1 and OCN was greater on poly(LLA-co-CL) and poly(LLA-co-DXO) than on poly(LLA) scaffolds.
Sculean, A., Nikolidakis, D. and Schwarz F. ( 2008). Regeneration of Periodontal Tissues: Combinations of Barrier Membranes and Grafting Materials - Biological Foundation and Preclinical Evidence: A Systematic Review, J. Clin. Periodontol., 35: 106-116.
2.
Bosshardt, D.D. (2008). Biological Mediators and Periodontal Regeneration: A Review of Enamel Matrix Proteins at the Cellular and Molecular Levels, J. Clin. Periodontol., 35: 87-105.
3.
Eickholz, P. , Krigar, D.M., Pretzl, B., Steinbrenner, H., Dorfer, C. and Kim, T.S. (2004). Guided Tissue Regeneration with Bioabsorbable Barriers. II. Long-term Results in Infrabony Defects, J. Periodontol., 75: 957-965.
4.
Trombelli, L. and Farina, R. (2008). Clinical Outcomes with Bioactive Agents Alone or in Combination with Grafting or Guided Tissue Regeneration , J. Clin. Periodontol., 35: 117-135.
5.
Elangovan, S. , Srinivasan, S. and Ayilavarapu , S. (2009). Novel Regenerative Strategies to Enhance Periodontal Therapy Outcome, Expert Opin. Biol. Ther., 9: 399-410.
6.
Rocchietta, I., Fontana, R. and Simion, M. (2008). Clinical Outcomes of Vertical Bone Augmentation to Enable Dental Implant Placement: A Systematic Review, J. Clin. Periodontol., 35: 203-215.
Tyson, T., Finne-Wistrand, A. and Albertsson, A.C. ( 2009). Degradable Porous Scaffolds from Various L-lactide and Trimethylene Carbonate Copolymers Obtained by a Simple and Effective Method , Biomacromolecules, 10: 149-154.
9.
Stjerndahl, A., Finne-Wistrand , A., Albertsson, A.C., Backesjo, C.M. and Lindgren, U. (2008). Minimization of Residual Tin in the Controlled Sn(II)octoate-catalyzed Polymerization of Epsilon-Caprolactone, J. Biomed. Mater. Res. A, 87: 1086-1091.
10.
Hakkarainen, M., Höglund, A., Odelius, K. and Albertsson, A.C. (2007). Tuning the Release Rate of Acidic Degradation Products through Macromolecular Design of Caprolactone-based Copolymers, J. Am. Chem. Soc., 129: 6308-6312.
11.
Koc, A., Emin, N., Elcin, E. and Elcin, Y.M. ( 2008). In Vitro Osteogenic Differentiation of Rat Mesenchymal Stem Cells in a Microgravity Bioreactor, J. Bioactive Compat. Polym., 23: 244-261.
12.
Hutmacher, D. , Hurzeler, M.B. and Schliephake , H. (1996). A Review of Material Properties of Biodegradable Polymers and Devices for GTR and GBR Applications, Int. J. Oral Maxillofac. Implants , 11: 667-678.
13.
Hutmacher, D. (2000). Scaffolds in Tissue Engineering Bone and Cartilage, Biomaterials, 21: 2529-2543.
14.
Andronova, N. and Albertsson, A.C. (2006). Resilient Bioresorbable Copolymers Based on Trimethylene Carbonate, L-lactide and 1,5-Dioxepan-2-one , Biomacromolecules, 7: 1489-1495.
15.
Odelius, K. , Plikk, P. and Albertsson, A.C. (2005). Elastomeric Hydrolyzable Porous Scaffolds Copolymers of Aliphatic Polyesters and a Polyether-Ester , Biomacromolecules, 6: 2718-2725.
16.
Ryner, M. and Albertsson, A.C. ( 2002). Resorbable and Highly Elastic Block Copolymers from 1,5-Dioxepan-2-one and L-lactide with Controlled Tensile Properties and Hydrophilicity, Biomacromolecules, 3: 601-608.
17.
Plikk, P., Odelius, K., Hakkarainen, M. and Albertsson, A.C. ( 2006). Finalizing the Properties of Porous Scaffolds of Aliphatic Polyesters through Radiation Sterilization, Biomaterials, 27: 5335-5347.
18.
Odelius, K. , Plikk, P. and Albertsson, A.C. (2008). The Influence of Composition of Porous Copolyester Scaffolds on Reactions Induced by Irradiation Sterilization , Biomaterials, 29: 129-140.
19.
Danmark, S. , Wistrand, A.F., Wendel, M., Arvidson, K., Albertsson, A.C. and Mustafa, K. (2010). Osteogenic Differentiation in Rat Bone Marrow Derived Stromal Cells on Customized Biodegradable Polymer Scaffolds, J. Bioactive Compat. Polym.,25: 207-223.
20.
Piche, J.E. , Carnes Jr, D.L. and Graves, D.T. (1989). Initial Characterization of Cells Derived from Human Periodontia, J. Dent. Res., 68: 761-767.
21.
Piche, J.E. and Graves, D.T. (1989). Study of the Growth Factor Requirements of Human Bone-derived Cells: A Comparison with Human Fibroblasts , Bone, 10: 131-138.
22.
Beresford, J.N., Gallagher, J.A., Poser, J.W. and Russell, R.G. (1984). Production of Osteocalcin by Human Bone Cells In Vitro. Effects of 1,25(OH)2D3, 24,25(OH)2D3, Parathyroid Hormone, and Glucocorticoids, Metab. Bone Dis. Relat. Res. , 5: 229-234.
23.
Mustafa, K. , Wroblewski, J., Hultenby, K., Lopez, B.S. and Arvidson, K. (2000). Effects of Titanium Surfaces Blasted with TiO2 Particles on the Initial Attachment of Cells Derived from Human Mandibular Bone. A Scanning Electron Microscopic and Histomorphometric Analysis, Clin. Oral Implants Res., 11: 116-128.
24.
Wataha, J.C., Craig, R.G. and Hanks C.T. (1992). Precision of and New Methods for Testing In Vitro Alloy Cytotoxicity, Dent. Mater., 8: 65-70.
25.
Eppley, B.L., Pietrzak, W.S. and Blanton, M.W. ( 2005). Allograft and Alloplastic Bone Substitutes: A Review of Science and Technology for the Craniomaxillofacial Surgeon, J. Craniofac. Surg., 16: 981-989.
26.
Charles-Harris, M., Koch, M.A., Navarro, M., Lacroix, D., Engel, E. and Planell, J.A. (2008). A PLA/Calcium Phosphate Degradable Composite Material for Bone Tissue Engineering: An In Vitro Study, J. Mater. Sci. Mater. Med., 19: 1503-1513.
27.
Pamula, E., Bacakova, L., Filova, E., Buczynska, J., Dobrzynski, P., Noskova, L. et al. (2008). The Influence of Pore Size on Colonization of Poly(L-lactide-glycolide) Scaffolds with Human Osteoblast-like MG 63 Cells In Vitro, J. Mater. Sci. Mater. Med., 19: 425-435.
28.
Vitale-Brovarone, C., Verne, E., Robiglio, L., Appendino, P., Bassi, F., Martinasso, G. et al. (2007). Development of Glass-Ceramic Scaffolds for Bone Tissue Engineering: Characterisation, Proliferation of Human Osteoblasts and Nodule Formation, Acta Biomater. , 3: 199-208.
29.
Frosch, K.H. , Barvencik, F., Viereck, V., Lohmann, C.H., Dresing, K., Breme, J. et al. (2004). Growth Behavior, Matrix Production, and Gene Expression of Human Osteoblasts in Defined Cylindrical Titanium Channels, J. Biomed. Mater. Res. A, 68: 325-334.
30.
Salasznyk, R.M., Williams, W.A., Boskey, A., Batorsky, A. and Plopper, G.E. (2004). Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells, J. Biomed. Biotechnol., 1: 24-34.
31.
Gercek, I., Tigli, R.S. and Gumusderelioglu, M. ( 2008). A Novel Scaffold Based on Formation and Agglomeration of PCL Microbeads by Freeze-drying, J. Biomed. Mater. Res. A, 86: 1012-1022.
32.
Chen, X.S., Zhang, Y., Wang, J.S., Li, X.Y., Cheng, X.K., Zhang, Y. et al. (2007). Diverse Effects of Stat1 on the Regulation of hsp90alpha Gene Under Heat Shock, J. Cell Biochem., 102: 1059-1066.
33.
Shi, S. , Cheng, X., Wang, J., Zhang, W., Peng, L. and Zhang, Y. (2009). RhBMP-2 Microspheres-loaded Chitosan/Collagen Scaffold Enhanced Osseointegration: An Experiment in Dog , J. Biomater. Appl., 23: 331-346.
34.
Zhang, Y., Cheng, X., Wang, J., Wang, Y., Shi, B., Huang, C. et al. (2006). Novel Chitosan/Collagen Scaffold Containing Transforming Growth Factor-Beta1 DNA for Periodontal Tissue Engineering, Biochem . Biophys. Res. Commun., 344: 362-369.
35.
Marinucci, L. , Lilli, C., Baroni, T., Beccchetti, E., Belcastro, S., Balducci, C. et al. (2001). In Vitro Comparison of Bioabsorbable and Non-resorbable Membranes in Bone Regeneration, J. Periodontol., 72: 753-759.
36.
Petrovic, L., Schlegel, A.K., Schultze-Mosgau, S. and Wiltfang, J. ( 2006). Different Substitute Biomaterials as Potential Scaffolds in Tissue Engineering, Int. J. Oral Maxillofac. Implants, 21: 225-231.
