Spherically supported bilayer membranes on cationic polymer beads were prepared from naturally occurring phospholipids (phosphatidylcholine (PC) and phosphatidylserine (PS)). The amount of phospholipids bound to the cationic polymer beads from the liposomal suspensions of various lipid compositions increased with increases in anionic PS. Confocal fluorescence microscopic observations with a fluorescence membrane probe indicated that the phospholipid molecules in the phospholipid-bead complexes were along the surface of the beads. Negatively charged trypan blue dye was preferably bound to the phospholipid-free beads by electrostatic attractive interaction, but was excluded from the phospholipid-bead complexes due to the bilayer membrane structure formed on the beads. This method could provide a better mimetic of biological membranes in comparison to physically unstable conventional liposomes.
Bangham, A.D. , Standishl, M.M. and Weissmann , G. (1965). The Action of Steroids and Streptolysin S on the Permeability of Phospholipid Structures to Cations, J. Mol. Biol., 13: 253-259.
2.
Evans, E.A. ( 1974). Bending Resistance and Chemically Induced Moments in Membrane Bilayers, Biophys. J., 14: 923-931.
3.
Lipowsky, R. ( 1991). The Conformation of Membranes, Nature , 349: 475-481.
4.
Sackmann, E. ( 1996). Supported Membranes: Scientific and Practical Applications , Science, 271: 43-48.
5.
Miyata, H. and Hotani, H. ( 1992). The Morphological Changes in Liposomes Caused by Polymerization of Encapsulated Actin Bundles, Proc. Nat. Acad. Sci. U. S. A., 89: 11547-11551.
6.
Mathivest, L. , Cribier, S. and Devaux, P.F. (1996). Shape Change and Physical Properties of Giant Phospholipid Vesicles Prepared in the Presence of an AC Electric Field, Biophys. J., 70: 1112-1121.
7.
Mengaer, F.M. and Keiper, J.S. (1998). Chemistry and Physics of Giant Vesicles as Membrane Models, Curr. Opin. Chem. Biol. , 2: 726-732.
8.
Yamashita, Y. , Oka, M., Tanaka, T. and Yamazaki, M. (2002). A New Method for the Preparation of Giant Liposomes in High Salt Concentrations and Growth of Protein Microcrystals in Them, Biochem. Biophys. Acta, 1561: 129-134.
Bayeri, T.M. and Bloom M. (1990). Physical Properties of Single Phospholipid Bilayers Adsorbed to Micro Glass Beads, Biophys. J., 58: 357-362.
11.
Korlach, J. , Schwille, P., Webb, W.W. and Feigenson , G.W. (1999). Characterization of Lipid Bilayer Phases by Confocal Microscopy and Fluorescence Correlation Spectroscopy, Proc. Nat. Acad. Sci. U. S. A., 96: 8461-8466.
12.
Buranda, T. , Huang, J., Ramarao, G.V., Ista, L.K., Larson, R.S., Ward, T.L. et al. (2003). Biomimetic Molecular Assemblies on Glass and Mesoporous Silica Microbeads for Biotechnology, Langmuir, 19: 1654-1663.
13.
Baksh, M.M. , Jaros, M. and Groves, J.T. (2004). Detection of Molecular Interactions at Membrane Surfaces Through Colloid Phase Transitions, Nature, 427: 139-141.
14.
Troutier, A.-L. and Ladavière , C. (2007). An Overview of Lipid Membrane Supported by Colloidal Particles, Adv. Colloid Interface Sci., 133: 1-21.
15.
Loose, M. and Schwille, P. ( 2009). Biomimetic Membrane Systems to Study Cellular Organization , J. Struct. Biol., 168: 143-151.
16.
Gopalakrishnan, G., Rouiller, I., Colman, D.R. and Lennox, R.B. (2009). Supported Bilayers Formed from Different Phospholipids on Spherical Silica Substrates, Langmuir, 25: 5455-5458.
17.
Schonherr, H. , Johnson, J.M., Lenz, P., Frank, C.W. and Boxer, S.G. (2004). Vesicle Adsorption and Lipid Bilayer Formation on Glass Studied by Atomic Force Microscopy, Langmuir, 20: 11600-11606.
18.
Reimhult, E., Hook, F. and Kasemo, B. ( 2003). Intact Vesicle Adsorption and Supported Biomembrane Formation from Vesicles in Solution: Influence of Surface Chemistry, Vesicle Size, Temperature, and Osmotic Pressure, Langmuir, 19: 1681-1691.
19.
Richter, R.P. , Berat, R. and Brisson, A.R. (2006). Formation of Solid-supported Lipid Bilayers: An Integrated View, Langmuir, 22: 3497-3505.
20.
Haratake, M., Hidaka, S., Ono, M. and Nakayama, M. ( 2006). An Ionic Polymer Bead-supported Lipid System, J. Colloid Interface Sci., 299: 924-927.
21.
Haratake, M. , Hidaka, S., Ono, M. and Nakayama, M. (2007). Preparation of an Ion-exchangeable Polymer Bead Wrapped with Bilayer Membrane Structures for High Performance Liquid Chromatography, Anal. Chim. Acta, 589: 76-83.
22.
Kitson, R.E. and Mellon, M.G. (1944). Colorimetric Determination of Phosphorus as Molybdivanadophosphoric Acid, Ind. Eng. Chem. Anal. Ed., 16: 379-383.
23.
Haratake, M. and Sunamoto, J. (2006). Direct Fusion between Poly(Ethylene Oxide)-lipid Modified Liposomes and Murine Mitotic B16 Melanoma Cells, J. Bioact. Compat. Polym., 21: 503-517.
24.
McAteer, J.A. and Davis, J. (1994). Basic Cell Culture Technique and the Maintenance of Cell Lines, In: Davis J.M. (ed.), Basic Cell Culture: A Practical Approach, IRL Press, Oxford, pp. 128.
25.
Whirtmarsh, A.J. and Davis, R.J. (1998). Structural Organization of MAP-kinase Signaling Modules by Scaffold Proteins in Yeast and Mammals, Trends Biochem. Sci., 23: 481-485.
