A rapid prototyping and rapid tool technique-based method was developed to fabricate chitosan fiber calcium phosphate cement composites (CF/CPC) for bone tissue engineering scaffold applications. The products were characterized and the in vitro performance with canine bone marrow stem cells (BMCs) on CF/CPC scaffold with controlled fiber structures evaluated. The X-ray diffraction analysis showed that about 91% of the inorganic part of the CF/CPC scaffold was hydroxyapatite (HA) and the variation in CF had little effect on the percentage of HA content. The results from in vitro study demonstrated that the interconnected macropores rapidly formed inside the CF/CPC scaffolds and that the patterns were related to the fiber structures used. The differences in the fiber structures altered the morphology of the BMCs without affecting the proliferation of the BMCs.
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3.
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4.
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7.
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8.
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9.
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12.
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16.
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17.
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18.
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19.
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20.
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21.
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22.
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23.
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24.
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25.
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26.
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27.
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28.
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29.
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30.
XuH.H.K., and SimonC.G. (2004). Self-hardening Calcium Phosphate Composite Scaffold for Bone Tissue Engineering, Journal of Orthopaedic Research, 22(3): 535–543.
31.
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32.
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34.
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35.
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36.
RyuW., MinS.W., HammerickK.E., VyakarnamM., GrecoR.S., PrinzF.B. (2007). The Construction of Three-Dimensional Micro-fluidic Scaffolds of Biodegradable Polymers by Solvent Vapor Based Bonding of Micro-molded Layers, Biomaterials, 28(6): 1174–1184.
