A cell encapsulation device based on the principle of high-voltage dispersion technology was designed and constructed. The parameters that influenced the formation of cell microcapsules, such as; voltage, push speed, electrode distance, and syringe size were analyzed and optimized. Unlike the traditional cell microencapsulating devices, both of the electrodes were separated from the CaCl2 solution in this device, which simplified the procedure of keeping the operation within a sanitary environment. Integrated adipose derived stem cell (ADSC) microcapsules with smooth surface and consistent diameter distribution were prepared. After 1-week in culture as a microcapsule and 3 days assembled in a construct, the ADSCs maintained a high viability and significant proliferation that established a favorable basis for the controlled assembly of cell microcapsules.
LimF., and SunA.M. (1980). Microencapsulated Islets as Bioartificial Endocrine Pancreas, Science, 210: 908–910.
3.
O'sheaG.M., and SunA.M. (1986). Encapsulation of Rat Islets of Langerhans Prolongs Xenograft Survival in Diabetic Mice, Diabetes, 35: 943–946.
4.
PrakashS., and JonesM.L. (2005). Artificial Cell Therapy: New Strategies for the Therapeutic Delivery of Live Bacteria, J. Biomed. Biotechnol., 1: 44–56.
5.
ChickW.L., PernaJ.J., LaurisV., LowD., GallettiP.M., PanolG. (1977). Artificial Pancreas Using Living Beta Cells: Effects on Glucose Homeostasis in Diabetic Rats, Science, 197: 780–782.
6.
SoonshiongP., HeintzR.E., MeridethN., YaoQ.X., YaoZ.W., ZhengT.L. (1994). Insulin Independence in a Type I Diabetic Patient after Encapsulated Islet Transplantation, Lancet, 343: 950–951.
7.
HumesH.D., BuffingtonD.A., MacKayS.M., FunkeA.J., WeitzelW.F. (1999). Replacement of Renal Function in Uremic Animals with a Tissue-engineered Kidney, Nat. Biotechnol., 17: 451–455.
8.
GrandosoL., PonceS., ManuelI., ArrueA., Ruiz-OrtegaJ.A., UlibarriI. (2007). Long-term Survival of Encapsulated GDNF Secreting Cells Implanted Within the Striatum of Parkinsonized Rats, Int. J. Pharm., 343: 69–78.
9.
MuruaA., De CastroM., OriveG., HernandezR.M., PedrazJ.L. (2007). In Vitro Characterization and In Vivo Functionality of Erythropoletin-secreting Cells Immobilized in Alginate-poly-L-lysine-alginate Microcapsules, Biomacromolecules, 8: 3302–3307.
10.
TaoW., WenR., GoddardMB., ShermanS.D., O'RourkeP.J., StabilaP.F. (2002). Encapsulated Cell-based Delivery of CNTF Reduces Photoreceptor Degeneration in Animal Models of Retinitis Pigmentosa, Invest. Ophthalmol. Vis. Sci., 43: 3292–3298.
11.
XuW., WangX.H., YanY.N., ZhengW., XiongZ., LinF. (2007). Rapid Prototyping Three-dimensional Cell/Gelatin/Fibrinogen Constructs for Medical Regeneration, J. Bioact. Compat. Polym., 22(4): 363–377.
12.
YaoR., ZhangR.J., YanY.N., and WangX.H. (2009). In Vitro Angiogenesis of Three-dimensional Tissue Engineered Adipose Tissues, J. Bioact. Compat. Polym., 24: 5–24.
WangX.H., YanY.N., and ZhangR.J. (2007). Rapid Prototyping as a Tool for Manufacturing Bioartificial Livers, Trends Biotechnol., 25: 505–513.
15.
XuW., WangX.H., YanY.N., and ZhangR.J. (2008). Rapid Prototyping of Polyurethane for the Creation of Vascular Systems, J. Bioact. Compat. Polym., 23(1): 103–114.
16.
YanY.N., WangX.H., XiongZ., LiuH.X., LiuF., LinF. (2005). Direct Construction of a Three-dimensional Structure with Cells and Hydrogel, J. Bioact. Compat. Polym., 20(3): 259–269.
17.
XuM.E., YanY.N., LiuH.X., YaoR., and WangX.H.Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique, J. Bioact. Compat. Polym., 24(S1): 31–47.
18.
LiS.J., YanY.N., XiongZ., WengC.Y., ZhangR.J., and WangX.H.Gradient Hydrogel Construct Based on an Improved Cell Assembling System, J. Bioact. Compat. Polym., 24(S1): 84–99.
19.
LiS.J., XiongZ., WangX.H., YanY.N., LiuH.X., and ZhangR.J.Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology, J. Bioact. Compat. Polym., 24(3): 249–265.
20.
ZhangT., YanY.N., WangX.H., XiongZ., LinF., WuR.D. (2007). Three-dimensional Gelatin and Gelatin/Hyaluronan Hydrogel Structures for Traumatic Brain Injury, J. Bioact. Compat. Polym., 22: 19–29.
21.
WangX.H., YanY.N., PanY.Q., XiongZ., LiuH.X., ChengB. (2006). Generation of Three-dimensional Hepatocyte/Gelatin Structures with Rapid Prototyping System, Tissue Eng., 12: 83–90.
22.
YanY.N., WangX.H., PanY.Q., LiuH.X., ChengJ., XiongZ. (2005) Fabrication of Viable Tissue-engineered Constructs with 3D Cell-assembly Technique, Biomaterials, 26: 5864–5871.
23.
LiuZ.C., and ChangT.M.S. (2002). Transplantation of Coencapsulated Hepatocytes and Marrow Stem Cells into Rats, Artif. Cells Blood Substit. Immobil Biotechnol., 30: 99–112.
24.
LiuZ.C., and ChangT.M.S. (2003). Coencapsulation of Stem Cells and Hepatocytes: In Vitro Conversion of Ammonia and In Vivo Studies on the Lowering of Bilirubin in Gunn Rats After Transplantation, Int. J. Artif. Organs, 26: 491–497.
