Aminophenyl boronic acid (APBA) modified hydrogel beads were prepared as a new sorbent for nucleotide isolation. Spherical hydrogel beads, obtained by suspension copolymerization, were the base material for the sorbent. The carboxyl groups on the gel bead surface were activated with a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). APBA was then covalently attached to the activated structure via the amine groups. The maximum APBA attached to the gel was 34mg/g. The reversible adsorption-desorption behavior of β-nicotinamide adenine dinucleotide (β-NAD) was investigated by using 3.5 and 34mg/g of APBA on the hydrogel beads. The equilibrium -NAD adsorption capacities for these beads were determined as approximately 25 and 100mg/g, respectively. The -NAD absorption capacity of these APBA beads is significantly greater than similar supports.
Crooke, S.T. (1992). Therapeutic Applications of Oligonucleotides , Ann. Rev. Pharmacol. Toxicol., 32: 329-376 .
2.
Hecker, K.H. , Green, S.M. and Kobayashi, K. (2000). Analysis and Purification of Nucleic Acids by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography , J. Biochem. Biophys. Methods, 46: 83-93 .
3.
Bruce, I.J. , Davies, M.J. , Howard, K. , Smethurst, D.E. and Todd, M. (1996). Magnetizable Solid-Phase Supports for Purification of Nucleic Acids , J. Pharm. Pharmacol., 48: 147-149 .
4.
Arshady, R. (1991). Beaded Polymer Supports and Gels. II. Physico-Chemical Criteria and Functionalization , J. Chrom. A, 586: 199-219 .
5.
Bergamann, C. , Müller-Schulte, D. , Oster, J. , Brassard, L. and Lübbe, A.S. (1999). Magnetic Ion-Exchange Nano- and Microparticles for Medical, Biochemical and Molecular Biological Applications , J. Magn. Magn. Mat., 194: 45-52 .
6.
Horak, D. , Smigol, V. , Labsky, J. , Svec, F. and Pilar, J. (1992). An E.P.R. Study of the Effect of Suspension Polymerization Conditions on the Properties of Glycidyl Methacrylate-Ethylene Dimethacrylate Particles , Polymer, 33: 2051-2056 .
7.
Horak, D. , Svec, F. , Tennikova, T.B. and Nahunek, M. (1992). Chromatographic Properties of Macroporous Beads from Poly(GMA-co-EDMA) , Agnew. Makromol. Chem., 195: 139-150 .
8.
Horak, D. , Labsky, J. , Pilar, J. , Bleha, M. , Pelzbauer, Z. and Svec, F. (1993). The effect of Polymeric Porogen on the Properties of Macroporous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) , Polymer, 34: 3481-3489 .
9.
Horak, D. , Straka, J. , Schneider, B. , Lednicky, F. and Pilar, J. (1994). Poly(ethylene dimethacrylate) Particles with Poly(glycidyl methacrylate) Functionalities , Polymer, 35: 1195-1202 .
10.
Malmsten, M. and Larsson, A. (2000). Immobilization of Trypsin on Porous Glycidyl Methacrylate Beads: Effects of Polymer Hydrophilization , Coll. Surf. B, 18: 277-284 .
11.
Senel, S. , Çiçek, H. and Tuncel, A. (1998). Production and Characterization of Poly(ethylene glycol dimethacrylate-styrene-glycidyl methacrylate) Microbeads , J. Appl. Polym. Sci., 67: 1319-1334 .
12.
Seitz, W.R. , Rooney, M.T.V. , Miele, E.W. , Wang, H. , Kaval, N. , Zhang, L. , Doherty, S. , Milde, S. and Lenda, J. (1999). Derivatized, Swellable Polymer Microspheres for Chemical Transduction , Anal. Chim. Acta, 400: 55-64 .
13.
Rooney, M.T.V. and Seitz, W.R. (1999). An Optically Sensitive Membrane for pH Based on Swellable Polymer Microspheres in Hydrogel , Analytical Communications, 36: 267-270 .
14.
Bahar, T. and Tuncel, A. (1999). Monodisperse Poly(p-chloromethylstyrene) Microbeads by Dispersion Polymerization , Polym. Eng. Sci., 39: 1849-1855 .
15.
Elmas, B. , Çamli, S.T. , Tuncel, M. , Senel, S. and Tuncel, A. (2001). DNA-Responsive Uniform Latex Particles Based on p-Chloromethylstyrene , J. Biomat. Sci. Polym. Ed., 12: 283-296 .
16.
Ünsal, E. , Bahar, T. , Tuncel, M. and Tuncel, A. (2000). DNA Adsorption onto Polyethyleneimine-attached Poly(p-chloromethylstyrene) Beads , J. Chrom. A, 898: 167-177 .
17.
Davies, M.J. , Smethurst, D.E. , Howard, K.M. , Todd, M. , Higgins, L.M. and Bruce, I.J. (1997). Improved Manufacture and Application of an Agarose Magnetizable Solid-Phase Support , Appl. Biochem. Biotech., 68: 95 .
18.
Schott, H. (1972). Book Review: Nucleic Acids , Angew. Chem. Internat. Edit., 11: 947 .
19.
Schott, H. , Rudloff, E. , Schmidt, P. , Roychoudhury, R. and Kössel, H. (1973). A Dihydroxyboryl-Substituted Methacrylic Polymer for the Column Chromatographic Separation of Mononucleotides, Oligonucleotides, and Transfer Ribonucleic Acid , Biochemistry, 12: 5 .
20.
Gehrke, C.W. , Kuo, K.C. , Davis, G.E. and Suits, R.D. (1978). Quantitative High-Performance Liquid Chromatography of Nucleosides in Biological Materials , J. Chromatogr., 150: 455-476 .
21.
Bouriotis, V. , Galpin, I.J. and Dean, P.D.G. , (1981). Applications of Immobilised Phenylboronic Acids as Supports for Group-Specific Ligands in the Affinity Chromatography of Enzymes , J. Chromatogr., 210: 267-278 .
22.
Vidal, P. , Deckert, T. , Hansen, B. and Welinder, B.S. (1989). High-Performance Liquid Chromatofocusing and Column Affinity Chromatography of In Vitro C-14 Glycated Human Serum Albumin. Demonstration of a Glycation-Induced Anionic Heterogeneity . J. Chromatogr., 476: 467-475 .
23.
Hjerten, S. and Li, J.P. (1990). High-Performance Liquid Chromatography of Proteins on Deformed Non-Porous Agarose Beads. Fast Boranate Affinity Chromatography of Haemoglobin at Neutral pH , J. Chromatogr., 510: 543-553 .
24.
Müller-Schulte, D. and Brunner, H. (1995). Novel Magnetic Microspheres on the Basis of Poly(vinyl alcohol) as Affinity Medium for Quantitative Detection of Glycated Haemoglobin , J. Chrom. A, 711: 53-60 .
25.
Koyama, T. and Terauchi, K. (1996). Synthesis and Application of Boronic Acid-Immobilized Porous Polymer Particles: A Novel Packing for High-Performance Liquid Affinity Chromatography , J. Chrom. B, 679: 31-40 .
26.
Singh, N. and Willson, R.C. (1999). Boranate Affinity Adsorption of RNA: Possible Role of Conformational Changes , J. Chrom. A, 840: 205-213 .
27.
Li, Y. , Pfüller, U. , Larsson, E.L. , Jungvid, H. , Galaev, I.Y. and Mattiasson, B. (2001). Separation of Mistletoe Lectins Based on the Degree of Glycosylation Using Boronate Affinity Chromatography , J. Chromatogr., 925: 115-121 .
28.
Tuncel, A. and Çiçek, H. (2000). 2-Hydroxypropylmethacrylate Based Mono- and Bifunctional Gel Beads Prepared by Suspension Polymerization , Polymer Int., 49: 485-494 .
29.
Maestas, R.R. , Prieto, J.R. , Kuehn, G.D. and Hageman, J.H. (1980). Polyacrylamide-boronate Beads Saturated with Biomolecules: A New General Support for Affinity Chromatography of Enzymes , J. Chromatogr., 189: 225-231 .
30.
Tuncel, A. and Özdemir, A. (2000). Thermally Reversible VPBA-NIPAM Copolymer Gels for Nucleotide Adsorption , J. Biomat. Sci. Polym. Ed., 11: 817-831 .
31.
Özdemir, A. and Tuncel, A. (2000), Boronic Acid Functionalized HEMA-based Gels for Nucleotide Adsorption , J. Appl. Polym. Sci., 78: 268-277 .
32.
Camli, S.T. , Senel, S. and Tuncel, A. (2002). Nucleotide Isolation by Boronic Acid Functionilized Hydrophilic Supports , Coll. Surf. A: Physicochemical and Engineering Aspects, 207: 127-137 .
33.
Senel, S. , Çamli, S.T , Tuncel, M. and Tuncel, A. (2002). Nucleotide Adsorption-Desorption Behaviour of Boronic Acid Functionalized Uniform-Porous Particles , J. Chrom. B., 769: 283-295 .
34.
Uguzdogan, A. , Denkbas, E.B. and Tuncel, A. (2002). RNA-Sensitive NIsopropylacrylamide/Vinylphenylboronic Acid Random Copolymer , Macromol. Biosci., 2: 214-222 .
35.
Elmas, B. , Onur, M.A. , Senel, S. and Tuncel, A. (2002). Temperature Controlled RNA Isolation by N-Isopropylacrylamide-Vinylphenyl Boronic Acid Copolymer Latex , Colloid Polym. Sci., 280: 1137-1146 .
36.
Elmas, B. , Onur, M.A. , Senel, S. and Tuncel A. (2004). Thermosensitive NIsopropylacrylamide-Vinylphenyl Boronic Acid Copolymer Latex Particles for Nucleotide Isolation , Coll. Surf. A, 232: 253-259 .
37.
Bahar, T. and Tuncel, A. (2000) Immobilization of -Chymotrypsin onto Newly Produced Poly(hydroxypropyl methacrylate-co-methacrylic acid) Hydrogel Beads , React. Funct. Polym., 44: 71-78 .
