Responses of Osteo- and Fibroblast Cells to Phosphorylated Chitin

Abstract

The cell responses of rat osteoblasts and fibroblasts to water-soluble phosphorylated chitin (P-chitin) were examined in vitro. Cell morphology was observed at 1, 4 and 7 days, respectively. A wide range of measurements, including the percentages of viable cells, total intracellular protein content, and 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide (MTT) assay on osteoblasts and fibroblasts, as well as alkaline phosphatase activity assay on osteoblasts at the end of 7 days culture, were conducted. P-chitin had no cytotoxity on either cell lines. An increase of viability and protein content of both cell types was found in media containing 0.10 g/mL of P-chitin after 7 days culture. The positive effects were especially significant for osteoblasts. Water-soluble P-chitin could be a promising biomaterial for tissue engineering.

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