Regional Differences in the Critical Period Neurodevelopment in the Mouse: Implications for Neonatal Seizures

Abstract

The voltage-sensitive calcium channel probe ¹²⁵I-ω-GVIA conotoxin has been shown to be a developmental marker in whole brain preparations of Swiss Webster mice. The present study looks more carefully at regional dissections of the mouse brain (cerebrum, cerebellum, and brain stem) at postnatal day 8 and postnatal day 16. ¹²⁵I-ω-GVIA conotoxin binding, thought to be presynaptic, showed a dramatic increase between postnatal days 8 and 16 in the cerebral cortex, a decrease in the cerebellum, and no change in the brain stem. The dramatic cerebral cortex increases indicated by these binding data correspond to a critical period between postnatal day 11 and postnatal day 14 in Swiss Webster mice; during this critical period, dendrites exhibit rapid outgrowth, sensory modalities come on line, electroencephalographic patterns mature, and the cortex reaches adult proportions. This period parallels a similar initiation of electrical maturation in the 28- to 32-week neonatal human brain. We conclude from these data that the unusual clinical presentation of neonatal seizures is not just the result of immature myelin formation. It includes incomplete synapse formation linking the cortex to the brain stem. (J Child Neurol 1994;9:77-80).

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References

Fenichel GM : Seizures, in Neonatal Neurology, 3rd ed. New York, Churchill Livingstone , 1990, pp 17-34.

Mizrahi EM , Kellaway P. : Characterization and classification of neonatal seizures. Neurology 1987;37:1837-1844.

Himwich WA : Biochemical and neurophysiological development of the brain in the neonatal period. Int Rev Neurobiol 1962;4:117-157.

Fenichel GM : Electroencephalography and evoked response, in Neonatal Neurology , 3rd ed. New York , Churchill Livingstone, 1990 , pp 225-252.

Litzinger MJ , Grover BB , Saderup S. , Abbott JR : Voltage sensitive calcium channels mark a critical period in mouse neurodevelopment. Int J Dev Neurosci , 1993;11:17-24.

Azimi-Zonooz A. , Litzinger MJ : The developing chick brain shows a dramatic increase in the ω-conotoxin binding sites around the hatching period . Int J Dev Neurosci 1992; 10:447-451.

Rivier JG , Galyean R. , Gray WR , et al: Neuronal calcium channel inhibitors. J Biol Chem 1987;262:1194-1198.

Cruz LJ , Olivera BM : Calcium channel antagonists. J Biol Chem 1986 ;261:6230-6233.

Bylund DB , Yamamura HI : Methods in receptor binding, in Yamamura HI , et al (eds): Methods in Neurotransmitter Receptor Analysis . New York, Raven Press, 1990, pp 1-35.

10.

Cruz LJ , Johnson DS , Olivera BM : Characterization of the ω-conotoxin target. Evidence for tissue-specific heterogeneity in calcium channel types. Biochemistry 1987;26:820-824.

11.

Smith PK , Krohn RI , Hermanson GT , et al: Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76-85.

12.

Kater SB , Mattson MP , Cohan C. , Connor J. : Calcium regulation of the neuronal growth cone. Trends Neurosci 1988;11:315-321.

13.

Cohen MW , Jones OT , Angelides KJ : Distribution of Ca2+ channels on frog motor nerve terminal revealed by fluorescent ω-conotoxin . J Neurosci 1991;11:1032-1039.

14.

Meyer FB , Anderson RE , Sundt TM Jr : Anticonvulsant effects of dihydropyridine Ca2+ antagonists in electrocortical shock seizures. Epilepsia 1990;31:68-74.

15.

Robitaille R. , Adler EM , Charlton MP : Strategic location of calcium channels at transmitter release sites of frog neuromuscular synapses . Neuron 1990;5:773-779.

16.

Yuste R. , Peinado A. , Katz LC : Neuronal domains in developing neocortex. Science 1992;257:665-669.

17.

Rihn LL , Claiborne BJ : Dendritic growth and regression in rat dentate granule cells during late postnatal development. Brain Res Dev Brain Res 1990;54:115-124.

18.

Fox K. , Daw N. , Sato H. , Czepita D. : The effect of visual experience on development of NMDA receptor synaptic transmission in kitten visual cortex. J Neurosci 1992;12:2672-2684.

19.

Kater SB , Mattson MP , Cohan C. , Connor J. : Calcium regulation of the neuronal growth cone. Trends Neurosci 1988;11:315-321.

20.

Kater SB , Mills LR : Regulation of growth cone behavior by calcium. J Neurosci 1991;11: 891-899.

21.

Murphy TH , Worley PF , Baraban JM : L-type voltage-sensitive calcium channels mediate synaptic activation of immediate early genes. Neuron 1991;7:625-635.

22.

Gamble E. , Koch C. : The dynamics of free calcium in dendritic spines in response to repetitive synaptic input. Science 1987;236:1311-1314.

23.

Murphy BJ , Rogers CA , Sunahara RK , et al: Identification, characterization, and photoaffinity labeling of the dihydropyridine receptor associated with the L-type calcium channel from bovine adrenal medulla. Mol Pharmacol 1990;37:173-181.

24.

Nowycky MC , Fox AP , Tsien RW : Three types of neuronal calcium channels with different calcium agonist sensitivity . Nature 1985;316:440-443, Proc Natl Acad Sci USA 1985;82:2178-2182.

25.

Wasterlain CG , Hattori H. , Yang C. , et al: Selective vulnerability of neuronal subpopulations during ontogeny reflects discrete molecular events associated with normal brain development, in Wasterlain CG , Vert P (eds): Neonatal Seizures. New York , Raven Press, 1990, pp 69-79.

26.

Soderfeldt B. , Fujikawa DG , Wasterlain CG : Neuropathology of status epilepticus in the neonatal marmoset monkey, in Wasterlain CG , Vert P (eds): Neonatal Seizures. New York , Raven Press, 1990, pp 91-98.