The role of compound eyes in photoperiodic regulation of diapause and entrainment of circadian rhythms was studied in a hemipteran, Graphosoma lineatum. Bilateral removal of compound eyes resulted in the loss of locomotor rhythmicity under both 24-h light cycle and constant darkness. The arrhythmicity was associated with the loss of photoperiodic regulation of diapause. Females with removed compound eyes had similar incidence of oviposition under diapause preventing long-day photoperiod (light:dark [LD] 18:6) and diapause inducing short-day photoperiod (LD 12:12). By contrast, both locomotor rhythmicity and photoperiodic regulation of diapause were maintained after unilateral removal of compound eyes. The results suggest that the compound eye–optic lobe axis is involved in the transmission of light signals for both entrainment of rhythms in locomotor activity and photoperiodism. The compound eyes probably act as photoreceptors in both transmission chains. Sites of the clocks for the two phenomena are discussed.
Balkenohl M and Weber F (1981) Sind auch bei holometabolen Insekten circadiane Schrittmacher der Aktivität in den optischen Ganglien localisiert?Mitt Deutsch Gesell Allg Angew Ent3:223-227.
2.
Binkley S (1993) Structures and molecules involved in generation and regulation of biological rhythms in vertebrates and invertebrates. Experientia49:648-653.
3.
Bowen MF , Saunders DS, Bollenbacher WE, and Gilbert LI (1984) In vitro reprogramming of the photoperiodic clock in an insect brain-retrocerebral complex. Proc Natl Acad Sci USA81:5881-5884.
4.
Claret J (1966) Mise en evidence du rôle de photorecepteur du cerveau dans l’induction de la diapause chez Pieris brassicae (Lepido). Ann Endocrinol27:311-320.
5.
Cymborowski B , Lewis RD, Hong S-F, and Saunders DS (1994) Circadian locomotor activity rhythms and their entrainment to light-dark cycles continue in flies (Calliphora vicina) surgically deprived of their optic lobes. J Insect Physiol40:501-510.
6.
Ferenz H-J (1975) Photoperiodic and hormonal control of reproduction in male beetles, Pterostichus nigrita. J Insect Physiol2:331-341.
7.
Ferrell BR and Reitcheck BG (1993) Circadian changes in cockroach ommatidial structure. J Comp Physiol A173:549-555.
8.
Fleissner G (1982) Isolation of an insect circadian clock. J Comp Physiol149:311-316.
9.
Hall JC (1996) Are cycling gene products as internal zeitgebers no longer the zeitgeist of chronobiology?Neuron17:779-802.
10.
Hasegawa K and Shimizu I (1987) In vivo and in vitro photoperiodic induction of diapause using isolated brain-suboesophageal ganglion complexes of the silkworm, Bombyx mori. J Insect Physiol33:959-966.
11.
Helfrich C (1987) Use of Drosophila melanogaster brain mutants for localization of the pacemaker of circadian locomotor activity rhythm. J Neurogenet4:137-140.
12.
Helfrich C , Cymborowski B, and Engelmann W (1985) Circadian activity rhythm in the house fly continues after optic tract severance and lobectomy. Chronobiol Intern2:19-22.
13.
Hodkova M (1976) Nervous inhibition of corpora allata by photoperiod in Pyrrhocoris apterus. Nature263:521-523.
14.
Kasai M and Chiba Y (1987) Effects of optic lobe ablation on circadian activity in the mosquito, Culex pipiens pallens. Physiol Entomol12:59-65.
15.
Lees AD (1964) The location of the photoperiodic receptors in the aphid Megoura viciae. J Exp Biol41:119-133.
16.
Mori H and Tomioka K (1994) Localization of circadian pacemaker within the optic lobe and its output pathway in the cricket. Zool Sci11(Suppl):110-110.
17.
Morita A and Numata H (1997) Distribution of photoperiodic receptors in the compound eyes of the bean bug, Riptortus clavatus. J Comp Physiol A180:181-185.
18.
Nakamura K , Hodek I, and Hodkova M (1996) Recurrent photoperiodic response in Graphosoma lineatum (Heteroptera: Pentatomidae). Eur J Entomol93:512-523.
19.
Nishiitsutsuji-Uwo J and Pittendrigh CS (1968) Central nervous system control of circadian rhythmicity in the cockroach. II: The pathway of light signals that entrain the rhythm. Z Vergl Physiol58:1-13.
20.
Numata H and Hidaka T (1983) Compound eyes as the photoperiodic receptors in the bean bug. Experientia39:868-869.
21.
Page TL (1978) Interaction between bilaterally paired components of the cockroach circadian system. J Comp Physiol124:225-236.
22.
Page TL (1985) Clocks and circadian rhythms. In Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol 6, GA Kerkut and LI Gilbert, eds, pp 577-652, Pergamon, Oxford, UK.
23.
Roberts SK (1965) Photoreception and entrainment of cockroach activity rhythms. Science148:958-959.
24.
Roberts SK (1974) Circadian rhythms in cockroaches: Effects of optic lobe lesions. J Comp Physiol88:21-30.
Saunders DS and Cymborowski B (1996) Removal of optic lobes of adult blow flies (Calliphora vicina) leaves photoperiodic induction of larval diapause intact. J Insect Physiol42:807-811.
27.
Shiga S and Numata H (1996) Effect of compound eye-removal on the photoperiodic response of the band-legged ground cricket, Pteronemobius nigrofasciatus. J Comp Physiol A179:625-633.
28.
Shiga S and Numata H (1997) Induction of reproductive diapause via perception of photoperiod through the compound eyes in the adult blow fly, Protophormia terraenovae. J Comp Physiol A181:35-40.
29.
Sokolove PG (1975) Localization of the cockroach optic lobe circadian pacemaker with microlesions. Brain Res87: 13-21.
30.
Sokolove PG and Loher W (1975) Role of eyes, optic lobes and pars intercerebralis in locomotory and stridulatory circadian rhythm of Teleogryllus commodus. J Insect Physiol21:785-799.
31.
Tomioka K and Chiba Y (1984) Effects of nymphal stage optic nerve severance or optic lobe removal on the circadian locomotor rhythm of the cricket Gryllus bimaculatus. Zool Sci1:375-382.
32.
Tomioka K and Ikeda M (1993) Involvement of serotonin in the circadian rhythm of an insect visual system. Naturwissenschaften80:137-139.
33.
Truman JW (1972) Physiology of insect rhythms. II: The silk moth brain as the location of the biological clock controlling eclosion. J Comp Physiol81:99-114.
34.
Truman JW (1974) Physiology of insect rhythms. IV: Role of the brain in the regulation of the flight rhythm of the giant silkmoths. J Comp Physiol95:281-296.
35.
Vivien-Roels B , Pevet P, Beck O, and Fevre-Montange M (1984) Identification of melatonin in the compound eyes of an insect, the locust (Locusta migratoria) by radioimmunoassay and gas chromatography–mass spectrometry. Neurosci Lett49:153-157.
36.
Waddell B , Lewis RD, and Engelmann W (1990) Localization of the circadian pacemakers of Hemideina thoracica (Orthoptera: Stenopelmatidae). J Biol Rhythms5:131-139.
37.
Williams CM and Adkisson PL (1964) Physiology of insect diapause. XIV: An endocrine mechanism for the photoperiodic control of pupal diapause in the oak silkworm, Antheraea pernyi. Biol Bull127:511-525.
