Changes in Lys- and Leu-aminopeplidase activities in several brain regions of the rat, after acute toluene administration, are described in this research. Aminopeptidase activity has been suggested as a candidate regulator of the degradation of several neuroactive peptides. Lys-aminopeptidase activity was significantly decreased in the thalamus, amygdala, and medulla oblongata. Leu-aminopeptidase activity was significantly decreased in the thalamus and cerebellum. It is suggested that these aminopeptidase activities could play a part in the mechanism of toluene neurotoxicity.
AlbaF.IribarC.RamirezM.ArenasC. (1989). “A fluorimetrie method for the determination of brain aminopeptidascs.”Arch. Neurobiol. 52:169–173.
2.
American Petroleum Institute. (1980). “A 26 week inhalation study of toluene in the rat” API Report 28–31210, Washington, D.C.
3.
BenignusV.A. (1981). “Neurobehavioral effects of toluene: A review.”Neurobehav. Toxicol. Teratol. 3:407–415.
4.
BjornaesS.NaalsundL.U. (1988). “Biochemical changes in different brain areas after toluene inhalation.”Toxicology49:367–374.
5.
BenuckM.MarksN. (1975). “Enzymatic inactivation of substance P by a partially purified enzyme from rat brain.”Biochem. Biophys. Res. Commun. 65:153–160.
6.
BradfordM.M. (1976). “A rapid and sensitive method for the quantitation of microgram protein utilizing the principle of protein-dye binding.”Analy. biochem. 72:248–254.
7.
CamargoA.C.M.Ramacho-PintoF.J.GreeneL.J. (1972). “Brain peptidases: Conversion and inactivation of kinin hormones.”J. Neurochem. 19:37–39.
8.
Von EulerG.FuxeK.BenfenatiF.HanssonT.AgnatiL.F.GustafssonJ.-A. (1989). “Neurotensin modulates the binding characteristics of dopamine D2 receptors in rat striatal membranes also following treatment with toluene.”Acta Physiol. Scand. 135:443–448.
9.
De GandariasJ.M.CasisL.MugicaR.ArechagaG.RamirezM. (1989). “Aging and brain aminopeptidase activity.”Rev. esp. Fisiol. 45:89–94.
10.
De GandariasJ.M.RamirezM.IribarC.ZulaicaJ.CasisL. (1989). “Postnatal development of aminopeptidase (arylamidase) activity in rat brain.”J. Biochem. 105:44–46.
11.
De GandariasJ.M.CasisO.IrazustaJ.EchevarriaE.CasisL. (1992a). “Effect of subacute benzene exposure on the activity of two neuropeptide-degrading enzymes in the rat brain.”Arch. Environ. Contam. Toxicol. 22:345–348.
12.
De GandariasJ.M.EchevarriaE.IrazustaJ.CasisO.CasisL. (1992b). “Regional distribution of neuropeptide-degrading enzyme activity in the rat brain. Effects of subacute exposure to carbon disulfide.”J. Biochem. Toxicol. 7:171–175.
13.
De GandariasJ.M.EchevarriaE.IrazustaJ.GilJ.CasisL. (1993). “Brain aminopeptidase activity after subacute xylene exposure.”Neurotoxicol. Teratol. 15:51–53.
14.
GospeS.M.Jr.CalabanM.J. (1988). “Central nervous system distribution of inhaled toluene.”Fund. Appl. Toxicol. 11:540–545.
15.
GreenbergL.J. (1962). “Fluorimetric measurement of alkaline phosphatase and aminopeptidase activities in the order of 10−14 mole.”Biochem. Biophys. Res. Commun. 9:430–435.
16.
HershL.B. (1982). “Degradation of enkephalins: The search for an enkephalinase.”Mol. Cell. Biochem. 47:35–43.
17.
JohnsonG.D.HershL.B. (1990). “Studies on the subsite specificity of the rat brain puromycin-sensitive aminopeptidase.”Arch. Biochem. Biophys. 273:305–309.
18.
KellyJ.A.NeidleE.L.NeidleA. (1983). “An aminopeptidase from mouse brain cytosol that cleaves N-terminal acidic aminoacid residues.”J. Neurochem. 40:1727–1733.
19.
KriegerD.T.BrownsteinM.J.MartinJ.B. (1983). “Brain Peptides.”A Wiley-Interscience Publication. John Wiley & Sons, New York.
20.
MarksN.DattaR.K.LajthaA. (1968). “Partial resolution of brain arylamidases and aminopeptidases.”J. Biol. Chem. 243:2882–2889.
21.
O'CuinnG.O'ConnorB.ElmoreM. (1990). “Degradation of Thyrotropin-Releasing Hormone and Luteinising Hormone-Releasing Hormone by enzymes of brain tissue.”J. Neurochem. 54:1–13.
22.
ReaT.M.NashJ.F.ZabikJ.E.BornG.S.KesslerW.V. (1984). “Effects of toluene inhalation on brain biogenic amines in the rat.”Toxicology31:143–150.
