The assessment of complex mixtures of environmental pollutants requires new interdisciplinary strategies. Integration of bioassay methodologies into these strategies is an important tool that pro vides direct evidence of the toxicity of a mixture. Short-term genetic bioassays are now widely used in the cancer assessment of complex mixtures. New and emerging interdisciplinary methodolo gies for assessing complex mixtures using bioassays are illustrated by the U.S. EPA's Integrated Air Cancer Project (IACP). The goals of this project are to identify the major airborne carcinogens and their emission sources and to improve the methodology and data available for human exposure and risk assessment from air borne carcinogens. The research effort is focused primarily on characterizing the impact of complex mixtures of products of incomplete combustion, including gaseous, semi-volatile, and particle-bound organic species. Short-term genetic bioassays have been integrated into several analysis strategies, including environ mental biomonitoring; bioassay-directed fractionation/characteri zation; transformation studies; source apportionment; and exposure, dosimetry, and risk assessment.
Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for detecting carcinogens and mutagens with the Salmonella mammalian microsome mutagenicity test. Mutation Res. 31:347-364.
2.
Burton, R.M., Marple, V.A., Liu, B.Y.H. and Johnson, B. (1987). A novel 2.5 mm cut virtual impactor for high volume PM10 sampling. In: Proceedings of the 1987 EPA/ APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 653-658, Air Pollution Control Association, Pittsburgh, Pa.
3.
Claxton, L.D. (1985). Assessment of bacterial bioassay methods for volatile and semi-volatile compounds and mixtures. Environ. Int.11:375-382.
4.
Claxton, L.D., Warren, S.H., Houk, V.S. and Lewtas, J. (1987). The mutagenicity of ambient and source samples from woodsmoke impacted airsheds. In: Proceedings of the 1987 EPA/APCA Symposium on Measurement of Toxic and Related Air Pollutants , pp. 591-596, Air Pollution Control Association, Pittsburgh, Pa.
5.
Cupitt, L.T., Claxton, L.D., Shepson, P.B. and Kleindienst, T.E. (1987). IACP emissions: transformations and fate. In: Proceedings of the 1987 EPA/APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 597-604, Air Pollution Control Association, Pittsburgh, Pa.
6.
Doll, R. and Peto, R. (1981). The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J. Natl. Cancer Inst.66:1191-
7.
Gordon, G. (1988). Receptor models. Environ. Sci. Technol.22:1132-1142.
8.
Harris, C.C. (1985). Future directions in the use of DNA adducts as internal dosimeters for monitoring human exposure to environmental mutagens and carcinogens. Environ. Health Perspect.62:185-191.
9.
Highsmith, V.R., Baumgardner, R.E., Drago, R.J., Lumpkin, T.A., Harris, D.B., Merrill, R.G. and Zweidinger, R.B. (1987). The collection of neighborhood air samples impacted by residential wood combustion in Raleigh, NC and Albuquerque, NM. Proceedings of the 1987 EPA/APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 562-572. Air Pollution Control Association, Pittsburgh, Pa.
10.
Highsmith, V.R., in press.
11.
Holmberg, B. and Alhborg, U. (1983). Consensus report: Mutagenicity and carcinogenicity of car exhausts and coal combustion emissions. Environ. Health Perspect.47:1-
12.
IARC (International Agency for Research on Cancer) (1985). Monograph programme on the evaluation of carcinogenic risk of chemicals to humans. Polynuclear Aromatic Compounds, Part 4, Bitumens, Coal-tar and Derived Products, Shale Oils and Soots, Vol. 35.
13.
Kado, N.Y., Langley, D. and Eisenstadt, E. (1983). A simple modification of the Salmonella liquis incubation assay: Increased sensitivity for detecting mutagens in human urine . Mutation Res. 121:25-32.
14.
Karch, N.J. and Schneiderman, M.A. (1981). Explaining the urban factor in lung mortality. National Resources Defense Council, Washington, DC.
15.
Kleindienst, T.E., Shepson, P.B., Edwey, E.O., Claxton, L. and Cupitt, L.T. (1986). Woodsmoke: Measurement of the mutagenic activities of its gas- and particulate-phase photooxidation products. Environ. Sci. Technol.20:493-501.
16.
Klouda, G.A., Currie, L.A., Sheffield, A.E., Wise, S.A., Benner, B.A., Stevens, R.K. and Merrill, R.G. (1987). The source apportionment of carbanaceous combustion products by micro-radiocarbon measurements for the integrated air cancer project (IACP). Proceedings of the 1987 EPA/ APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 573-578, Air Pollution Control Association, Pittsburgh, Pa.
17.
Lewis, C.W., Baumgardner, R.E., Claxton, L.D., Lewtas, J. and Stevens, R.K. (1988). The contribution of woodsmoke and motor vehicle emissions to ambient aerosol mutagenicity. Environ. Sci. Technol.22:968-971.
18.
Lewtas, J. (1985a). Combustion emissions: Characterization and comparison of their mutagenic and carcinogenic activity. In: Carcinogens and Mutagens in the Environment, Volume V, The Workplace: Sources of Carcinogens , pp. 59-74. CRC Press, Boca Raton, Fl.
19.
Lewtas, J. (1985b). Development of a comparative potency method for cancer risk assessment of complex mixtures using short-term in vivo and in vitro bioassays. Toxicol. Indust. Health1: 193-203.
20.
Lewtas, J. (1988). Genotoxicity of complex mixtures: Strategies for the identification and comparative assessment of airborne mutagens and carcinogens from combustion sources. Fund. Appl. Toxicol.10:571-589.
21.
Lewtas, J., Claxton, L. and Mumford, J. (1987). Human exposure to mutagens from indoor combustion sources. In: Indoor Air87, pp. 8-14. Vol. 3, Berlin, Oraniendruck GMBH.
22.
Lewtas, J., Claxton, L., Mumford, J. and Lofroth, G. (1989). Bioassay of complex mixtures of indoor air pollutants, IARC monographs on indoor air pollution methods, in press.
23.
Lewtas, J., Goto, S., Williams, K., Chuang, J.C., Petersen, B.A. and Wilson, N.K. (1987). The mutagenicity of indoor air particles in a residential pilot field study: Application and evaluation of new methodologies . Atmos. Environ.21:443-449.
24.
McCann, J., Choi, E., Yamasaki, E. and Ames, B.N. (1975). Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals. Proc. Natl. Acad. Sci. U.S.A.72:5135-5139.
25.
Merrill, R.G. (1987). Progress toward identifying source specific tracers . Proceedings of the 1987 EPA/APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 585-590, Air Pollution Control Association, Pittsburgh, Pa.
26.
NRC (National Research Council) (1988). Complex mixtures. National Academy Press, Washington, DC.
27.
Randerath, K., Randerrath, E., Agrawal, H.P., Gupta, R.C., Schurdak, E. and Reddy, M.V. (1985). Postlabeling methods for carcinogen-DNA adduct analysis. Environ. Health Perspect.62:57-65.
28.
Report Of The Task Group. (1978). Air pollution and cancer: Risk assessment methodology and epidemiological evidence. Environ. Health Perspect.22:1-12.
29.
Schuetzle, D. and Lewtas, J. (1986). Bioassay-directed chemical analysis in environmental research. Anal. Chem.58:1060A-1075A.
30.
Stevens, R.K., Lewis, C.W., Dzubay, T.G., Baumgardner, R.E., Zweidinger, R.B., Highsmith, R.V., Cupitt, L.T., Lewtas, J., Claxton, L.D., Currie, L. and Zak, B. (1989). Mutagenic atmospheric aerosol sources apportioned by receptor modeling. Toxics in the Atmosphere, ASTM publication, in press.
31.
Zweidinger, R., Dropkin, D., Stump, F., Drago, R. and Tejada, S. (1987). Volatile organic hydrocarbon and aldehyde composition in Raleigh, North Carolina during the 1985 woodsmoke study. Proceedings of the 1987 EPA/APCA Symposium on Measurement of Toxic and Related Air Pollutants, pp. 579-584. Air Pollution Control Association , Pittsburgh, Pa.
