Pathologic Effects of Chronic Administration of Hydrochlorothiazide,with and without Sodium Nitrite,to F344 Rats

Abstract

The diuretic drug hydrochlorothiazide was administered to 24 male and 24 female F344 rats as a mixture of 0.1% in powdered food. A parallel group of the same size was given 0.1% hydrochlorothiazide plus 0.2% sodium nitrite in the food. A third group received 0.2% sodium nitrite in the food and there was a similar group of untreated controls. The treatments were well tolerated and there was no significant life shortening. A majority of the rats given hydrochlorothiazide, with or without nitrite, developed chronic progressive nephropathy, which was more severe in males than in females. Associated with this were diffuse parathyroid hyperplasia in both groups receiving the drug, also more severe in males than in females, and parallel increases in lesions of the blood vessels (mural thrombosis of the heart and polyarteritis). The few adenomas of the parathyroid and tubular cell adenomas of the kidney in rats ingesting hydrochlorothiazide were not statistically significant.

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References

Gold, B. and Mirvish, S.S. (1977). N-Nitroso derivatives of hydrochlorothiazide, nitridazole and tolbutamide. Toxicol. Appl. Pharmacol. 40:131-136.

Lehr, D. and Churg, J. (1952). Human and experimental arteriosclerosis. J. Mt. Sinai Hospital 19:106-120.

Lijinsky, W. and Epstein, S.S. (1970). Nitrosamines as environmental carcinogens. Nature (London) 225:21-23.

Rao, T.K. , Lijinsky, W. and Epler, J.L. (1984). Genotoxicology of N-nitroso compounds, pp. 249-250. New York: Plenum.

Reuber, M.D. (1977). Necropsy of animals for scientific research. Clinical Toxicology 10:111-127.

Reuber, M.D. (1980). The significance of acute and chronic renal disease in Osborne-Mendel rats ingesting dieldrin or aldrin. Clinical Toxicology 7:159-170.

Solleveld, M.-A. , Haseman, J.K. and Mcconnell, E.E. (1984). Natural history of body weight gain, survival, and neoplasia in the F344 rat. J. Natl. Cancer Inst. 72:929-940.