The glutathione and NADP + status of perfused rabbit lung was deter mined both before and after perfusion with 1 mM paraquat. The pul monary glutathione redox state was similar to that of liver, having a glutathione/glutathione disulfide ratio of about 240. This ratio was lower in lungs perfused with glucose-free medium, a condition in which NADPH probably becomes limiting. Perfusion with paraquat signifi cantly increased the pulmonary glutathione disulfide content, particu larly in the absence of glucose, resulting in a glutathionelglutathione disulfide ratio of <100. NADP+ levels in rabbit lung were increased approximately two-fold by perfusion with paraquat in medium con taining glucose and three-fold in the absence of glucose.
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