Evaluation of DNA damage in long-term paint exposed workers by non-invasive buccal micronucleus assay

Abstract

Workers involved in paint production or application are extensively exposed to various hazardous substances like organic solvents, lead-based pigments, adhesives, and residual plastic monomers. Therefore, workers in the paint industry are at high risk of suffering adverse health effects. Studies of the lymphocytes of paint workers have demonstrated that industrial paint induces DNA damage and cellular changes. The aim of the present study was to assess DNA damage in 54 paint workers from paint production and application areas and 54 age-gender matched control subjects using a non-invasive buccal micronucleus (MN) assay. Buccal MN frequencies were significantly increased in workers compared to controls. MN frequencies significantly increased among workers from paint production areas compared with workers from paint application areas. MN frequencies in long-term workers (>10 years) were found to be significantly higher than those of short-term workers (≤10 years), which indicates that the duration of exposure to paints causes cytogenetic damage. MN frequencies increased with increasing age, while smoking status and the use of protective masks had no additional effect on MN frequencies within groups. In conclusion, it appears that long-term exposure to complex chemical mixtures during paint production may increase DNA damage in the workers. Understanding the possible causes of occupational exposure-induced genotoxicity in paint industry workers is of great importance for the protection of public health. Monitoring variables related to genotoxic damage in the paint workers using non-invasive methods will facilitate and improve risk assessment in the paint production sector.