Monitoring metal levels in water and multiple biomarkers in the grouper ( Epinephelus tauvina ) to assess environmental stressors on the Arabian Gulf coast of Saudi Arabia

Abstract

In this field study, the levels of heavy metals (Pb, Fe, Co, Cu, and Zn) in water and a suite of biochemical and histological biomarkers in the grouper (Epinephelus tauvina) were assessed at four sites in the Arabian Gulf. Samples were taken from a relatively non-urban reference site, called Salwa (S1), and three effluent-dominated sites, namely Al-aziziyah in southern Dammam city (S2), the Al-Jubail coast (S3), and Manifa (S4). Toxic metals, namely Pb and Co (at all sites) and Fe (at S3), were elevated in water samples relative to the internationally permissible limits. In fish, induced levels of heat shock protein 70 (HSP70) in the liver at S3 and S4 were higher than those of the reference fish at S1. Additionally, the level of the lipid peroxidation (LPO) product (malondialdehyde (MDA)) was significantly increased in gills (at S3) and liver (at S2 and S3). There was an inhibition of catalase activities in the gills of fish from S2 to S4 and significantly higher activity levels of superoxide dismutase in the gills of fish from S4. Histopathological features such as aneurysms in gill vessels, deformed gill lamellae, increases in liver melano-macrophage centers, and hepatocellular necrosis were most abundant at sites where significant pollution problems exist (i.e. S2–S4). The results reveal that the eastern coast of Saudi Arabia, in the Arabian Gulf, is still contaminated, as indicated by elevated HSP70, LPO content and numbers of histological lesions, and that monitoring of contaminants and their effects should be continued in this region.