The aim of this study was to evaluate the effect of tocopherol on pleuritis-induced rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single TCDD dose of 5 μg/kg body weight (b.w.) and then for 3 weeks they were daily supplemented with tocopherol at a dose of 30 mg/kg b.w. The inflammation was initiated by intrapleural injection of a single dose of 1% carrageenin solution in a volume of 0.15 ml. Changes in biochemical blood parameters were measured three times at the 24th, 72nd and 120th hour of pleuritis and the blood was collected from 20 animals of each group of rats (group with the control inflammation; group treated with TCDD and with control inflammation; group treated with TCDD, supplemented with tocopherol and with the inflammation). The following biochemical parameters were measured: tumor necrosis factor (TNF), interleukin-1 (IL-1), IL-2, IL-4, IL-6, procollagen, telopeptide, fibrinogen, cholesterol, urea, creatinine, aspartate aminotransferase (AspAT) and alanine aminotransferase (AlAT). Daily supplementation of tocopherol caused significant changes in the level of TNF, IL-1, IL-4, IL-6, urea, creatinine, AspAT and AlAT. According to the results of these studies, we suggest that tocopherol supplementation in high doses could act as a protective treatment to improve liver metabolism.
AhmadNKhalidBLukeD. (2005) Tocotrienol offers better protection than tocopherol from free radical-induced damage of rat bone. Clinical and Experimental Pharmacology and Physiology32: 761–770.
2.
AiubCPintoLFelzenszwalbI (2008) DNA-repair genes and vitamin E in the prevention of N-nitrosodiethylamine mutagenicity. Cell Biology and Toxicology25: 393–402.
3.
AlsharifNHassounE (2004) Protective effects of vitamin A and vitamin E succinate against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced body wasting, hepatomegaly, thymic atrophy, production of reactive oxygen species and DNA damage in C57BL/6 J mice. Basic and Clinical Pharmacology and Toxicology95: 131–138.
4.
AlyHDomenechO (2009) Cytotoxicity and mitochondrial dysfunction of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in isolated rat hepatocytes. Toxicology Letters191: 79–87.
5.
AslamFKhanAKhanM (2009) Toxico-pathological changes induced by cypermethrin in broiler chicks: their attenuation with vitamin E and selenium. Experimental and Toxicologic Pathology62: 441–450.
6.
BoverhofDBurgoonLTashiroC. (2006) Comparative toxicogenomic analysis of the hepatotoxic effects of TCDD in Sprague Dawley rats and C57BL/6 mice. Toxicological Science94: 398–416.
7.
CałkosińskiI (2005) The course of experimentally induced acute pleuritis with use of nitrogranulogen (NTG) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Habilitation Thesis, Wroclaw University of Medicine, Wroclaw.
8.
CałkosińskiI (2008) The influence of tocopherol on diagnostic indexes of inflammatory reaction in rats undergoing dioxin exposition. Habilitation Thesis, Wroclaw University of Medicine, Poland.
CałkosińskiIDobrzyńskiMKobierska-BrzozaJ. (2010) The influence of strain, sex and age on selected biochemical parameters in blood serum of buffalo and Wistar rats. Polish Journal of Veterinary Sciences13: 293–299.
11.
CałkosińskiIRosińczuk-TonderysJDzierzbaK. (2011a) Estimation of the action of three different mechlorethamine doses on biochemical parameters during experimentally induced pleuritis in rats. Pharmacological Reports63: 501–517.
12.
CałkosińskiIRosińczuk-TonderysJSzopaM. (2011b) High doses of tocopherol in the prevention and potentiation of dioxin in experimental inflammation-potential application. Postepy Higieny Medycyny Doswiadczalnej65: 143–157.
13.
CapuronLMoranisACombeN. (2009) Vitamin E status and quality of life in the elderly: influence of inflammatory processes. British Journal of Nutrition102: 1390–1394.
14.
ChungJVeeramachaneniSLiuC. (2009): Vitamin E supplementation does not prevent ethanol-reduced hepatic retinoic acid levels in rats. Nutrition Research29: 664–670.
15.
ComitatoRNesaretnamKLeoniG. (2009) A novel mechanism of natural vitamin E tocotrienol activity: involvement of ERbeta signal transduction. American Journal of Physiology Endocrinology and Metabolism297: 427–437.
16.
De ArribaGDe HornedoJPRubioSR. (2009) Vitamin E protects against the mitochondrial damage caused by cyclosporin A in LLC-PK1 cells. Toxicology and Applied Pharmacology239: 241–250.
17.
DedeSMertHMertN. (2006) Effect of alpha-tocopherol on serum trace and major elements in rats with bleomycine induced pulmonary fibrosis. Biological Trace Element Research114: 175–184.
18.
DharajiyaNChoudhuryBBacsiA. (2007) Inhibiting pollen reduced nicotinamide adenine dinucleotide phosphate oxidase-induced signal by intrapulmonary administration of antioxidants blocks allergic airway inflammation. Journal of Allergy and Clinical Immunology119: 646–653.
19.
DrabkoKBojarska-JunakAKowalczykJ (2008) Activity of superoxide dismutase and glutathione peroxidase and concentrations of malonyldialdehyde, vitamin E, total antioxidant status and extracellular cytokines concentrations in children with acute lymphoblastic leukaemia. Medycyna Wieku Rozwojowego10: 861–868.
20.
El-DemerdashFYousefMKedwanyF. (2004) Role of alpha-tocopherol and beta-carotene in ameliorating the fenvalerate-induced changes in oxidative stress, hemato-biochemical parameters, and semen quality of male rats. Journal of Environmental Science and Health Part B39: 443–459.
21.
FowlerBLucierGBrownH. (1973) Ultrastructural changes in rat liver cells following a single oral dose of TCDD. Environmental Health Perspectives5: 141–148.
22.
GalliFCanestrariFBuoncristianiU (1999) Biological effects of oxidant stress in haemodialysis: the possible roles of vitamin E. Blood Purification17: 79–94.
23.
GrochowalskiAChrzaszczRPiekloR. (2001) Estrogenic and antiestrogenic effect of in vitro treatment of follicular cells with 2,3,7,8-tetrachlorodibenzo-p-dioxin. Chemosphere43: 823–827.
24.
HassounEVodhanelJAbushabanA (2004) The modulatory effects of ellagic acid and vitamin E succinate on TCDD-induced oxidative stress in different brain regions of rats after subchronic exposure. Journal of Biochemical and Molecular Toxicology18: 196–203.
25.
HassounEAVodhanelJHoldenB. (2006) The effects of ellagic acid and vitamin E succinate on antioxidant enzymes activities and glutathione levels in different brain regions of rats after subchronic exposure to TCDD. Journal of Toxicology and Environmental Health69: 381–393.
26.
HoriTKatafuchiTTakeS. (1995) The hypothalamo-sympatic nervous system modulates peripheral cellular immunity. Neurobiology3: 309–317.
27.
HoraiRAsanoMSudoK. (1998) Production of mice deficient in genes for interleukin (IL-1)-1alpha, IL-1β, Il-1alpha/beta and IL-1 receptor antagonist shows that IL-1β is crucial in turpentine-induced fever development and glucocorticoid secretion. Journal of Experimental Medicine3: 1463–1475.
28.
HsiehCCHuangCJLinBF (2006) Low and high levels of alpha-tocopherol exert opposite effects on IL-2 possibly through the modulation of PPAR-gamma, IkappaBalpha, and apoptotic pathway in activated splenocytes. Nutrition22: 433–440.
29.
HushmendySJayakumarLHahnAB. (2009) Select phytochemicals suppress human T-lymphocytes and mouse splenocytes suggesting their use in autoimmunity and transplantation. Nutrition Research29: 568–578.
30.
HwangSW (1973) Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the biliary excretion of indocyanide-green in rats. Environmental Health Perspectives5: 227–231.
31.
JailalIDeverajSVenugopolSK (2002) Oxidative stress, inflammation and diabetic vasculopathies: the role of alpha tocopherol therapy. Free Radical Research36: 1331–1336.
32.
KappusHDiplockAT (1992) Tolerance and safety of Vitamin E: a toxicological position report. Free Radical Biology and Medicine13: 55–74.
33.
KleinADeckertVSchneiderM. (2006) Alpha-tocopherol modulates phosphatidylserine externalization in erythrocytes: relevance in phospholipid transfer protein-deficient mice. Arteriosclerosis, Thrombosis and Vascular Biology26: 2160–2167.
34.
KobayashiYTazawaYNakagawaM. (1984) Ultrastructural changes in skeletal muscle of a patient with familial intrahepatic cholestasis associated with vitamin E deficiency. Tohoku Journal of Experimental Medicine142: 337–346.
35.
KojA (1974) Acute-phase reactants—their synthesis, turnover and biological significance. In: AllisonAC (ed) Structure and Function of Plasma Protein. Vol. 1. London, UK: Plenum Press, 1974, pp. 73–131.
36.
KwonEChoYDoGM. (2009) Actions of ferulic acid and vitamin E on prevention of hypercholesterolemia and atherogenic lesion formation in apolipoprotein E-deficient mice. Journal of Medical Food12: 996–1003.
37.
LamprechtMHofmannPGreilbergerJ. (2009) Increased lipid peroxidation in trained men after 2 weeks of antioxidant supplementation. International Journal of Sports Nutrition and Exercise Metabolism19: 385–399.
38.
LauritzenBLykkesfeldtJSkaanildM. (2003) Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae infection. Research in Veterinary Science74: 261–270.
39.
NanYWuWFuN. (2009) Antioxidants vitamin E and 1-aminobenzotriazole prevent experimental non-alcoholic steatohepatitis in mice. Scandinavian Journal of Gastroenterology44: 1121–1131.
40.
NearRMatulkaRMannK. (1999) Regulation of preB cell apoptosis by aryl hydrocarbon receptor/transcription factor-expressing stromal/adherent cells. Proceedings of the Society for Experimental Biology and Medicine221: 242–252.
41.
NeubertRJacob-MüllerUStahlmannR. (1990) Polyhalogenated dibenzo-p-dioxins and dibenzofurans and the immune system. 1. Effects on peripheral lymphocyte subpopulations of a non-human primate (Callithrix jacchus) after treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Archives of Toxicology64: 345–359.
42.
NorazlinaMLeePLukmanH. (2007) Effects of vitamin E supplementation on bone metabolism in nicotine-treated rats. Singapore Medical Journal48: 195–199.
43.
NovikovaPKrasil'nikovP (2009) Molecular modeling of the reactive configuration of peroxidized lipid and alpha-tocopherol in the membrane. Biofizika54: 675–680.
44.
PrasadK (2009) Vitamin E does not regress hypercholesterolemic atherosclerosis. Journal of Cardiovascular Pharmacology and Therapeutics14: 231–241.
45.
ServaisSLetexierDFavierR. (2007) Prevention of unloading-induced atrophy by vitamin E supplementation: links between oxidative stress and soleus muscle proteolysis?Free Radical Biology and Medicine42: 627–635.
46.
SinghUDevarajSJialalIVitaminE (2005), oxidative stress and inflammation. Annual Review of Nutrition25: 151–174.
47.
SinghUJialalI (2004) Anti-inflammatory effects of alpha-tocopherol. Annals of New York Academy of Sciences1031: 195–203.
48.
StanleyR (1971) Isomers of alpha-tocopheryl acetate and their biological activity. Lipids6: 281–356.
49.
TeravestPTytgatGWestraM. (2005) Haemolytic anaemia and a clotting disorder as first signs of cystic fibrosis in two infants. Nederlands Tijdschrift voor Geneeskunde149: 2125–2218.
50.
TsukamotoHRippeRNiemeläO. (1995) Roles of oxidative stress in activation of Kupffer and Ito cells in liver fibrogenesis. Journal of Gastroenterology and HepatologySuppl 1: 50–53.
51.
Van Den BergMBirnbaumLDenisonM. (2006) The 2005 world health organization reevaluation of human and mammalian toxic equivalency factors for dioxins and dioxin-like compounds. Toxicological Science93: 223–241.
52.
VilukselaMBagerYTuomistoJ. (2000) Liver tumor-promoting activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in TCDD-sensitive and TCDD-resistant rat strains. Cancer Research60: 6911–6920.
53.
WangSSunNZhangJ. (2002) Immunomodulatory effects of high-dose alpha-tocopherol acetate on mice subjected to sidestream cigarette smoke. Toxicology175: 235–245.
54.
WangXZhouXXuJ. (2009) The effects of vitamin E on NK cell activity and lymphocyte proliferation in treated mice by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Immunopharmacology and Immunotoxicology31: 432–438.
55.
WeglickiWStaffordRFreedmanA. (1993) Modulation of cytokines and myocardial lesions by vitamin E and chloroquite in a Mg-deficient rat model. American Journal of Physiology264: 723–726.
56.
WongMPortmannBSherwoodR. (2007) The cytoprotective effect of alpha-tocopherol and daidzein against d-galactosamine-induced oxidative damage in the rat liver. Metabolism56: 865–875.
57.
YamaokaSKimHOgiharaT. (2008) Severe vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation. Free Radical Research42: 602–612.
58.
ZhouJChenPZhouY. (2005) 3,4-Methylenedioxymethamphetamine (MDMA) abuse may cause oxidative stress and potential free radical damage. Free Radical Research37: 491–497.
