This study evaluates the application of low magnetic field (LMF) on inulinase enzyme production by Geotrichum candidum under solid state fermentation (SSF) using leek as potential carbon source. First, the fermentation conditions were optimized using normal magnetic field grown microorganism. Among eight G. candidum isolates, the most effective strain called G. candidum OC-7 was selected to use in further experiments. In the second part of the study, SSF was carried out under different LMFs (4 and 7 mT). The results showed that inulinase activity was strongly affected by LMF application. The highest enzyme activity was obtained as 535.2 U/g of dry substrate (gds) by 7 mT magnetic field grown G. candidum OC-7. On the contrary, the control had only 412.1 U/gds. Consequently, the use of leek presents a great potential as an alternative carbon source for inulinase production and magnetic field treatment could effectively be used in order to enhance the enzyme production.
AlegreACPPolizeliMDTDTerenziHF. (2009) Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source. Brazilian Journal of Microbiology40(3): 612–622.
2.
AlvarezDCPerezVHJustoOR. (2006) Effect of the extremely low frequency magnetic field on nisin production by Lactococcus lactis subsp lactis using cheese whey permeate. Process Biochemistry41(9): 1967–1973.
3.
AntovMNikolicMSrdicV. (2009) Immobilization of invertase from Saccharomyces cerevisiae on core/shell silica supports. New Biotechnology25: S126–S127.
4.
AouidiFKhelifiEAssesN. (2010) Use of cheese whey to enhance Geotrichum candidum biomass production in olive mill wastewater. Journal of Industrial Microbiology & Biotechnology37(8): 877–882.
5.
AyyachamyMKhelawanKPillayD. (2007) Production of inulinase by Xanthomonas campestris pv phaseoli using onion (Allium cepa) and garlic (Allium sativum) peels in solid state cultivation. Letters in Applied Microbiology45(4): 439–444.
6.
BurkertJFMKalilSJMaugeriF. (2006) Parameters optimisation for enzymatic assays using experimental design. Brazilian Journal of Chemical Engineering23(2): 163–170.
7.
CanliOErdalSTaskinM. (2011) Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis. Toxicology and Industrial Health27(1): 35–39.
8.
ChenHQChenXMLiY. (2009) Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06. Food Chemistry115(4): 1206–1212.
9.
ChiZMChiZZhangT. (2009) Inulinase-expressing microorganisms and applications of inulinases. Applied Microbiology and Biotechnology82(2): 211–220.
10.
CreangaDEPoiataAMorariuVV. (2004) Zero-magnetic field effect in pathogen bacteria. Journal of Magnetism and Magnetic Materials272-276: 2442–2444.
11.
DanialENElnasharMMMAwadGEA (2010) Immobilized inulinase on grafted alginate beads prepared by the one-step and the two-steps methods. Industrial & Engineering Chemistry Research49: 3120–3125.
12.
ErdalSTaskinM (2010) Production of alpha-amylase by Penicillium expansum MT-1 in solid-state fermentation using waste Loquat (Eriobotrya japonica Lindley) kernels as substrate. Romanian Biotechnological Letters15(3): 5342–5350.
13.
GeXYZhangWG (2005) A shortcut to the production of high ethanol concentration from Jerusalem artichoke tubers. Food Technology and Biotechnology43(3): 241–246.
14.
GromadaAFiedurekJ (1997) Selective isolation of Aspergillus niger mutants with enhanced glucose oxidase production. Journal of Applied Microbiology82(5): 648–652.
15.
JiWJHuangHMDengAH. (2009) Effects of static magnetic fields on Escherichia coli. Micron40(8): 894–898.
16.
KimCHRheeSK (1990) Ethanol-production from Jerusalem artichoke by inulinase and Zymomonas mobilis. Applied Biochemistry and Biotechnology23(2): 171–180.
17.
KohnoMYamazakiMKimuraII. (2000) Effect of static magnetic fields on bacteria: Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Pathophysiology7(2): 143–148.
18.
MahantaNGuptaAKhareSK (2008) Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresource Technology99(6): 1729–1735.
19.
MazuttiMBenderJPTreichelH. (2006) Optimisation of inulinase production by solid-state fermentation using sugarcane bagasse as substrate. Enzyme and Microbial Technology39: 56–59.
20.
MillerGL (1959) Use of dinitro salicylic acid reagent for determination of reducing sugar. Analytical Chemistry31(3): 426–428.
21.
MughalMSAliSAshiqM. (2009) Kinetics of an extracellular exo-inulinase production from a 5-flourocytosine resistant mutant of Geotrichum candidum using two-factorial design. Bioresource Technology100(14): 3657–3662.
22.
MurthyPSNaiduMMSrinivasP (2009) Production of α-amylase under solid-state fermentation utilizing coffee waste. Journal of Chemical Technology and Biotechnology84(8): 1246–1249.
23.
NakamuraTShitaraAMatsudaS. (1997) Production, purification and properties of an endoinulinase of Penicillium sp. TN-88 that liberates inulotriose. Journal of Fermentation and Bioengineering84: 313–318.
24.
NovakJStrasakLFojtL. (2007) Effects of low-frequency magnetic fields on the viability of yeast Saccharomyces cerevisiae. Bioelectrochemistry70(1): 115–121.
25.
OngenbaysalGSukanSSVassilevN (1994) Production and properties of inulinase from Aspergillus niger. Biotechnology Letters16(3): 275–280.
26.
OnionAHSAllsoppDEgginsHOW (1986) Smith’s Introduction to Industrial Mycology. New York: Wiley, 217–226.
27.
PahwaSKaurSJainR. (2010) Structure based design of novel inhibitors for histidinol dehydrogenase from Geotrichum candidum. Bioorganic & Medicinal Chemistry Letters20(13): 3972–3976.
28.
ReisAPGGuimarãesVMFerreiraJG. (2010) Hydrolysis of galacto-oligosaccharides in soy molasses by α-galactosidases and invertase from Aspergillus terreus. Brazilian Archives of Biology and Technology53(3): 719–729.
29.
RosesRPGuerraNP (2009) Optimisation of amylase production by Aspergillus niger in solid-state fermentation using sugarcane bagasse as solid support material. World Journal of Microbiology & Biotechnology25(11): 1929–1939.
30.
SahaBC (2006) Production of mannitol from inulin by simultaneous enzymatic saccharification and fermentation with Lactobacillus intermedius NRRL B-3693. Enzyme and Microbial Technology39: 991–995.
31.
SelvakumarPPandeyA (1999) Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus. Process Biochemistry34(8): 851–855.
32.
SguareziCLongoCCeniG. (2009) Inulinase production by agro-industrial residues: optimisation of pretreatment of substrates and production medium. Food and Bioprocess Technology2(4): 409–414.
33.
SharmaADKainthSGillPK (2006) Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp. Journal of Food Engineering77: 486–491.
34.
SinghRSDhaliwalRPuriM (2006) Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus. Process Biochemistry41: 1703–1707.
35.
SinghRSDhaliwalRPuriM (2007) Production of high fructose syrup from Asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1. Journal of Industrial Microbiology & Biotechnology34(10): 649–655.
36.
SinghaniaRRSukumaranRKPatelAK. (2010) Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme and Microbial Technology46: 541–549.
37.
StrasakLVetterlVSmardaJ (2002) Effects of low-frequency magnetic fields on bacteria Escherichia coli. Bioelectrochemistry55(1-2): 161–164.
38.
TaskinME (2010) Isolation of a reactive black-5 decolourizing fungus, Absidia californica MT-1, form cement-contained soil and the optimisation of culture conditions for dye removal. Romanian Biotechnological Letters22: 7123–7134.
39.
TaskinMECanliO (2010) Isolation of scleroglucan-producing fungus, Sclerotium rolfsii MT-6, from fermented swuash and the optimisation of submerged culture conditions for scleroglucan production. Asian Journal of Chemistry22: 7920–7926.
40.
VanlooJCoussementPDeleenheerL. (1995) On the presence of inulin and oligofructose as natural ingredients in the western diet. Critical Reviews in Food Science and Nutrition35(6): 525–552.
41.
WeiWLWanWGLeHY. (1999) Continuous preparation of fructose syrups from Jerusalem artichoke tuber using immobilized intracellular inulinase from Kluyveromyces sp Y-85. Process Biochemistry34: 643–646.
42.
XiongCWangJHLiDS (2007) Optimisation of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology. Biochemical Engineering Journal34: 179–184.
