The availability of bone powder as inorganic element source on growth and development in wheat seedlings

Abstract

Bone powder (BP), a by-product of cattle slaughtering plants, consists of fat, protein, amino acids, inorganic elements and vitamins. It is used as bone meal in biomedical and feed industry because of its rich content. In addition, bone contains a large amount of inorganic elements especially calcium (Ca) and phosphorus (P), which the plants need to maintain their physiological and biochemical processes. BP has been used as Ca and P source in agriculture for many years; however, its effects on plant growth and development have not yet been studied in detail. The present study aimed to determine the effects of BP on dry weight and contents of total chlorophyll, soluble protein and sugar as indicators of physiological response in the leaves. For this purpose, bone powder solutions (BPS) were prepared at different concentrations (0.5%, 1%, 1.5% and 2%) and applied to growing media of the 10-day wheat seedlings. Afterward, the 14-day seedlings were harvested, and the effects of BPS on plant response were determined. The results showed that BPS treatments significantly stimulated dry weight and contents of total chlorophyll, protein and sugar compared to control seedlings. The best stimulatory effect of BPS was determined at 1% concentration. According to these results, it is possible to say that BP may be used to meet the inorganic element requirements of plants in poor soils and hydroponic systems.

Keywords

Bone waste wheat inorganic element

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References

Andarwulan

Shetty

(1999) Influence of acetyl salicylic acid in combination with fish protein hydrolysates on hyperhydricity reduction and phenolic synthesis in oregano (Origanum vulgare) tissue cultures. Journal of Food Biochemistry 23(6): 619–635.

Bradford

(1976) A rapid and sensitive and method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Analytic Biochemistry 72: 248–254.

Cakmakci

Erdogan

(2005) Organik Tarım. Ispir Hamza Polat M.Y.O. Yayın No: 2. Erzurum: Atatürk Univ, 214.

Dumlupinar

Demir

Budak

Karabulut

Kadi

Karakurt

. (2007) Determination of replacement of some inorganic elements in pulvinus of bean (Phaseolus vulgaris cv. Gina 2004) at chilling temperature by the WDXRF spectroscopic technique. Journal of Quantitative Spectroscopy and Radiative Transfer 103(2): 331–339.

Dumlupinar

Genisel

Erdal

Korkut

Taspinar

Taskin

(2011) Effects of progesterone, β-estradiol, and androsterone on the changes of inorganic element content in barley leaves. Biological Trace Element Research. doi: 10.1007/s12011-011-8980-6. In press.

Elsheikh

EAE

Elzidany

(1997) Effects of Rhizobium inoculation, organic and chemical fertilizers on yield and physical properties of faba bean seeds. Plant Foods for Human Nutrition 51(2): 137–144.

Erdal

(2009) Effects of mammalian sex hormones on antioxidant enzyme activities, H₂O₂ content and lipid peroxidation in germinating bean seeds. Journal of the Faculty of Agriculture of Ataturk University 40(2): 79–85.

Erdal

Dumlupinar

(2010) Progesterone and β-estradiol stimulate the seed germination in chickpea by causing important changes in biochemical parameters. Zeitchrift fur Naturforschhung Section C 65(3–4): 239–244.

Erdal

Dumlupinar

(2011a) Mammalian sex hormones stimulate antioxidant system and enhance growth of chickpea plants. Acta Physiologiae Plantarum 33(3): 1011–1017.

10.

Erdal

Dumlupinar

(2011b) Exogenously treated mammalian sex hormones affects inorganic constituents of plants. Biological Trace Element Research. doi: 10.1007/s12011-010-8857-0. In press.

11.

Erdal

Dumlupinar

Cakmak

Taskin

(2010) Determination of some inorganic element concentration changes in germinating chickpea seeds exposed to progesterone and β-estradiol by using WDXRF spectroscopic technique. Fresenius Environmental Bulletin 19: 507–515.

12.

Erdal

Taskin

(2010a) Uptake of textile dye Reactive Black-5 by Penicillium chrysogenum MT-6 isolated from cement-contaminated soil. African Journal of Microbiology Research 4(8): 618–625.

13.

Erdal

Taskin

(2010b) Production of α-amylase by Penicillium expansum MT-1 in solid-state fermentation using waste Loquat (Eriobotrya japonica Lindley) kernels as substrate. Romanian Biotechnological Letters 15(3): 5342–5350.

14.

Genisel

(2010) Effects of bone powder solution, CaCl₂ and IAA applications on physiologic parameters of bean (Phaseolus vulgaris) under salt stress. Unpublished doctoral thesis, Ataturk University, Erzurum, Turkey.

15.

Hodge

Hofreiter

(1962) Determination of reducing sugars and carbohydrates. In: Wilster

Wolfrom

(eds) Methods in Carbohydrate Chemistry. New York, NY: Academic Press, 380–394.

16.

Kurbanoglu

Atici

Algur

(2004) Effect of ram horn hydrolyzate on the growth of bean (Phaseolus vulgaris cv. Aziziye-94). Biological Agriculture and Horticulture 22(2): 121–131.

17.

Lichtenthaler

Buschmann

(2001) Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In: Wrolstad

(ed) Current Protocols in Food Analytical Chemistry. New York, NY: John Wiley, F4.3.1–F4.3.8.

18.

Parida

Das

(2005) Prasanna Mohanty defense potentials to NaCl in a mangrove, Bruguiera parviflora: differential changes of isoforms of some antioxidative enzymes. Journal of Plant Physiology 161(8): 531–542.

19.

Price

Schweigert

(1971) The science of meat and meat products. 2nd Ed. San Francisco, CA: W. H. Freeman, 559–561.

20.

Taskin

Erdal

(2011) Production of carotenoids by Rhodotorula glutinis MT-5 in submerged fermentation using the extract from waste loquat kernels as substrate. Journal of the Science of Food and Agriculture 91(8): 1440–1445.

21.

Taskin

Erdal

Canli

(2010) Utilization of waste loquat (Eriobotrya Japonica Lindley) kernels as substrate for scleroglucan production by locally isolated Sclerotium rolfsii . Food Science and Biotechnology 19(4): 1069–1075.

22.

Taskin

Erdal

Genisel

(2011) Biomass and exopolysaccharide production by Morchella esculenta in submerged culture using the extract from waste loquat (Eriobotrya japonica Lindley) kernels. Journal of Food Processing and Preservation. doi: 10.1111/j.1745-4549.2010.00510.x. In press.